| BETHANY LUTHERAN HOMES INC | La Crosse | — | $40.6M | 7 |
| WISCONSIN PIPE TRADES HEALTH FUND | Milwaukee | — | $40.5M | 3 |
| MILWAUKEE INSTITUTE OF ART & DESIGN INC | Milwaukee | — | $40.2M | 5 |
| NORTHWESTERN MUTUAL LIFE FOUNDATION INC | Milwaukee | B82 Education | $40.1M | 0 |
| BENEVOLENT CORPORATION CEDAR COMMUNITY | West Bend | P750 Human Services | $40.1M | 7 |
| TRI-COUNTY MEMORIAL HOSPITAL INC | Whitehall | E22 Health Care | $39.9M | 13 |
| St Clare Memorial Hospital Inc | Oconto Falls | E22 Health Care | $39.8M | 9 |
| FOUR LAKES EDUCATION INC | McFarland | B99 Education | $39.6M | 6 |
| MOUNT MARY UNIVERSITY INC | Milwaukee | — | $39.5M | 11 |
| MARIAN UNIVERSITY INC | Fond Du Lac | B420 Education | $39.1M | 5 |
| CDA HOUSING INC | Kenosha | L40 Housing & Shelter | $38.6M | 0 |
| THE UNIVERSITY SCHOOL OF MILWAUKEE | Milwaukee | B200 Education | $38.5M | 8 |
| THEDACARE MEDICAL CENTER - NEW LONDON INC | Appleton | E22 Health Care | $38.5M | 7 |
| FUND FOR WISCONSIN SCHOLARS INC | Madison | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $38.4M | 8 |
| TRAVAUX INC | Milwaukee | L40 Housing & Shelter | $38.4M | 9 |
| POLK-BURNETT ELECTRIC COOPERATIVE | Centuria | — | $37.9M | 16 |
| PLANNED PARENTHOOD OF WISCONSIN INC | Milwaukee | E420 Health Care | $37.9M | 10 |
| NATIONAL CONFERENCE OF BAR EXAMINERS | Madison | I030 Crime & Legal-Related | $37.7M | 10 |
| 170 FREEDOM MILWAUKEE 2024 HOST | Milwaukee | S30 Community Improvement & Capacity Building | $37.6M | 3 |
| Gundersen Lutheran Medical Foundation Inc | La Crosse | E120 Health Care | $37.4M | 19 |
| MEMORIAL HOSPITAL INC | Neillsville | E22 Health Care | $37.3M | 3 |
| PLUMBERS AND STEAMFITTERS LOCAL 400 AND | Kaukauna | Y43 Mutual & Membership Benefit | $37.3M | 3 |
| Madison Surgery Center Inc | Middleton | E32 Health Care | $37.1M | 6 |
| MOUNDVIEW MEMORIAL HOSPITAL & CLINICS | Friendship | E220 Health Care | $36.9M | 12 |
| WELLPOINT CARE NETWORK INC | Milwaukee | Z99 Unknown | $36.8M | 9 |
| WISCONSIN EARLY CHILDHOOD ASSOCIATION | Fitchburg | — | $36.7M | 2 |
| CUMBERLAND MEMORIAL HOSPITAL INC | Cumberland | E22 Health Care | $36.4M | 14 |
| Chiara Communities Inc | Waukesha | L20 Housing & Shelter | $36.2M | 0 |
| MEMORIAL HOSPITAL OF BOSCOBEL | Boscobel | E22Z Health Care | $35.7M | 14 |
| SAINT JOHN'S COMMUNITIES INC | Milwaukee | P750 Human Services | $35.5M | 7 |
| YOUNG MEN'S CHRISTIAN ASSOCIATION OF THE FOX CITIES | Appleton | P270 Human Services | $35.2M | 9 |
| FLAMBEAU HOSPITAL INC | Park Falls | E220 Health Care | $35.1M | 3 |
| MILWAUKEE REPERTORY THEATER INC | Milwaukee | A650 Arts, Culture & Humanities | $34.8M | 6 |
| INTERNATIONAL FOUNDATION OF EMPLOYEE | Brookfield | S41Z Community Improvement & Capacity Building | $34.2M | 19 |
| SCENIC RIVERS ENERGY COOPERATIVE | Lancaster | — | $34.1M | 15 |
| BELLIN PSYCHIATRIC CENTER | Green Bay | F31Z Mental Health & Crisis Intervention | $33.6M | 13 |
| THE MORGRIDGE INSTITUTE | Madison | E20 Health Care | $33.6M | 23 |
| PMML NORTH INC | Kenosha | A54 Arts, Culture & Humanities | $33.4M | 3 |
| UNITED LUTHERAN PROGRAM FOR THE AGING | Wauwatosa | P750 Human Services | $33.4M | 9 |
| CARMEN HIGH SCHOOL OF SCIENCE AND | Milwaukee | B29 Education | $33.2M | 6 |
| SPOONER HEALTH SYSTEM INC | Spooner | E20 Health Care | $32.9M | 6 |
| WISCONSIN MILK MARKETING BOARD INC | Madison | — | $32.9M | 35 |
| RIPON MEDICAL CENTER INC | Ripon | E220 Health Care | $32.7M | 9 |
| MILWAUKEE JEWISH FEDERATION INC | Milwaukee | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $32.3M | 10 |
| Milwaukee Regional Medical Center Thermal Service Inc | Milwaukee | E112 Health Care | $32.3M | 2 |
| WISCONSIN HEALTH FUND | Milwaukee | — | $32.2M | 11 |
| MMAC COMMUNITY SUPPORT FOUNDATION INC | Milwaukee | S80Z Community Improvement & Capacity Building | $32M | 1 |
| MILWAUKEE CATHOLIC HOME INC | Milwaukee | P750 Human Services | $31.8M | 5 |
| ST CAMILLUS HEALTH CENTER INC | Wauwatosa | P750 Human Services | $31.6M | 7 |
| MCPREPARATORY SCHOOL | Milwaukee | B24P Education | $31.3M | 7 |
