| CAHABA MEDICAL CARE FOUNDATION | Centreville | E30 Health Care | $61.9M | 8 |
| HUDSON-ALPHA INSTITUTE FOR BIOTECHNOLOGY | Huntsville | U99 Science & Technology | $61.7M | 28 |
| Wiregrass Electric Cooperative | Hartford | — | $61.3M | 18 |
| THE COMMUNITY ACTION PARTNERSHIP OF NORTH ALABAMA INC | Decatur | T70Z Philanthropy, Voluntarism & Grantmaking Foundations | $60.9M | 2 |
| South Alabama Electric Cooperative | Troy | — | $59.3M | 14 |
| Coosa Valley Electric Cooperative Inc | Talladega | — | $58M | 15 |
| Feeding the Gulf Coast | Theodore | K31 Food, Agriculture & Nutrition | $57.5M | 1 |
| PEA RIVER ELECTRIC COOPERATIVE | Ozark | — | $56.4M | 16 |
| FAMILY SECURITY CREDIT UNION | Decatur | — | $54.9M | 6 |
| FIVE STAR CREDIT UNION | Dothan | — | $54.1M | 15 |
| RUSSELLVILLE HOSPITAL INC | Russellville | E22 Health Care | $53.7M | 7 |
| COMMUNITY FOOD BANK OF CENTRAL ALABAMA | Birmingham | K31Z Food, Agriculture & Nutrition | $53.6M | 1 |
| Methodist Home for the Aging dba Methodist Homes Corporation | Birmingham | P750 Human Services | $53M | 5 |
| ALABAMA PARTNERSHIP FOR CHILDREN | Montgomery | B60 Education | $51.5M | 1 |
| RETAIL WHOLESALE & DEPARTMENT STORE INTL UNION & INDUSTRY HEALTH & BENEFIT FUND | Birmingham | — | $50.9M | 1 |
| SOUTHERN RESEARCH INSTITUTE | Birmingham | U330 Science & Technology | $50.4M | 20 |
| Black Warrior Electric Membership Corporation | Demopolis | — | $48.9M | 16 |
| UNIVERSITY OF MOBILE | Mobile | B430 Education | $47.9M | 8 |
| CLARKE-WASHINGTON ELECTRIC MEMBERSHIP CORPORATION | Jackson | — | $46M | 17 |
| WEST ALABAMA AIDS OUTREACH INC FIVE HORIZONS HEALTH SERVICES | Tuscaloosa | — | $44.3M | 5 |
| St Vincent's Health System | Birmingham | E22 Health Care | $43.8M | 0 |
| FRANKLIN PRIMARY HEALTH CENTERS INC | Mobile | E320 Health Care | $42M | 27 |
| ARAB ELECTRIC COOPERATIVE INC | Arab | — | $41.6M | 10 |
| Talladega College | Talladega | — | $40.9M | 0 |
| BIRMINGHAM-SOUTHERN COLLEGE | Birmingham | — | $40.6M | 13 |
| GLENWOOD INC | Birmingham | — | $40.2M | 9 |
| MARINE ENVIRONMENTAL SCIENCES CONSORTIUM MS & R LLC | Dauphin Island | U120 Science & Technology | $39.5M | 10 |
| CAPSTONE HEALTH SERVICES FOUNDATION PC | Tuscaloosa | — | $39.4M | 18 |
| Food Bank of North Alabama Inc | Huntsville | K31Z Food, Agriculture & Nutrition | $39.1M | 1 |
| STERLING CO INC | Gadsden | Z99 Unknown | $37.8M | 0 |
| ELECTRIC COOPERATIVES HEALTH BENEFITS PLAN | Montgomery | Y43 Mutual & Membership Benefit | $37.1M | 2 |
| HUNTINGDON COLLEGE | Montgomery | B420 Education | $36.2M | 9 |
| EAST ALABAMA MEDICAL CENTER VOLUNTARY EMPLOYEE BENEFICIARY ASSOCIATION TRUST | Opelika | Y43 Mutual & Membership Benefit | $35.7M | 4 |
| DYNETICS INC EMPLOYEE HEALTH AND WELFARE PLAN | Huntsville | Y43 Mutual & Membership Benefit | $34.9M | 3 |
| The Arc of Central Alabama Inc | Birmingham | — | $34.8M | 4 |
| Miles College Inc | Fairfield | B42 Education | $34.2M | 11 |
| Heart of Alabama Food Bank | Montgomery | K31Z Food, Agriculture & Nutrition | $34M | 1 |
| AIDS ACTION COALITION OF HUNTSVILLE | Huntsville | G81Z Diseases, Disorders & Medical Disciplines | $33.1M | 8 |
| Pioneer Electric Cooperative Inc | Greenville | Y22 Mutual & Membership Benefit | $33M | 13 |
| THE LEGACY FOUNDATION | Montgomery | A12 Arts, Culture & Humanities | $32.8M | 5 |
| WALKER AREA COMMUNITY FOUNDATION INC | Jasper | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $32.6M | 2 |
| QUALITY OF LIFE HEALTH SERVICES INC | Gadsden | E39Z Health Care | $32.5M | 5 |
| THE YOUNG MEN'S CHRISTIAN ASSOCIATION OF BIRMINGHAM INC | Birmingham | P270 Human Services | $31.7M | 10 |
| SOUTH ALABAMA REGIONAL PLANNING COMMISSION | Mobile | S30 Community Improvement & Capacity Building | $31.7M | 5 |
| RANDOLPH SCHOOL | Huntsville | B24Z Education | $31.4M | 7 |
| THE COMMUNITY FOUNDATION OF GREATER BIRMINGHAM | Birmingham | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $30.6M | 5 |
| HUNTSVILLE EMERGENCY MEDICAL SERVICES INC | Huntsville | — | $30.6M | 3 |
| Alabama Forest Land Trust Inc | Mobile | — | $30.2M | 2 |
| GOODWILL INDUSTRIES OF THE GULF COAST INC | Mobile | J32Z Employment | $29.8M | 6 |
| SOUTHEAST ALABAMA RURAL HEALTH ASSOCIATES | Troy | E30 Health Care | $29.2M | 8 |
