| FLORIDA HEALTHY KIDS CORPORATION | Tallahassee | E800 Health Care | $250.8M | 9 |
| MEMORIAL HOSPITAL - WEST VOLUSIA INC | DeLand | E220 Health Care | $250.4M | 33 |
| FLORIDA HOSPITAL ZEPHYRHILLS INC | Zephyrhills | E220 Health Care | $246.7M | 33 |
| STETSON UNIVERSITY INC | DeLand | B430 Education | $245.2M | 20 |
| HILLSBOROUGH COUNTY SCHOOL READINESS | Tampa | B012 Education | $238.8M | 5 |
| Employee Welfare Benefit Plans Trust of FPL Group Inc & Its Affiliates | Juno Beach | — | $231.9M | 1 |
| CITRUS HEALTH NETWORK INC | Hialeah | — | $230.6M | 16 |
| ROLLINS COLLEGE | Winter Park | B420 Education | $226.8M | 17 |
| FLORIDA INSTITUTE OF TECHNOLOGY INC | Melbourne | B430 Education | $222.5M | 14 |
| FAMILY SUPPORT SERVICES OF | Jacksonville | P80 Human Services | $222.3M | 11 |
| The Villages Tri-County Medical Center Inc | The Villages | E220 Health Care | $221.3M | 33 |
| FARM SHARE INC | Homestead | K30Z Food, Agriculture & Nutrition | $220.8M | 3 |
| BayCare Home Care Inc | Largo | E92 Health Care | $218.8M | 13 |
| Baptist Medical Center of the Beaches Inc | Jacksonville | E220 Health Care | $218.8M | 12 |
| BIG BEND COMMUNITY BASED CARE INC | Tallahassee | E60 Health Care | $217.3M | 7 |
| ORANGE COUNTY SCHOOL READINESS | Orlando | B21 Education | $216.3M | 1 |
| SOLARIS FOUNDATION INC | Windermere | E91 Health Care | $215.6M | 13 |
| FAIRWINDS CREDIT UNION | Orlando | — | $214.5M | 13 |
| FEEDING AMERICA TAMPA BAY INC | Tampa | K31Z Food, Agriculture & Nutrition | $212.9M | 9 |
| Baptist Outpatient Services Inc | Coral Gables | E30 Health Care | $212.3M | 10 |
| CAMELOT COMMUNITY CARE INC | Clearwater | P200 Human Services | $210.9M | 11 |
| LADIES PROFESSIONAL GOLF ASSOCIATION | Daytona Beach | — | $208.8M | 22 |
| South Florida Baptist Hospital Inc | Plant City | E220 Health Care | $202.7M | 12 |
| THE UNIVERSITY OF FLORIDA FOUNDATION INC | Gainesville | B110 Education | $200.5M | 14 |
| STATE CHARTERED CREDIT UNIONS IN FLORIDA | Jonesville | — | $197.8M | 18 |
| CAPE CANAVERAL HOSPITAL INC | Rockledge | E220 Health Care | $197.7M | 13 |
| FLORIDA SOUTHERN COLLEGE | Lakeland | B420 Education | $195.7M | 11 |
| Barry University Inc | Miami Shores | B430 Education | $195M | 16 |
| ECKERD YOUTH ALTERNATIVES INC | Clearwater | P30 Human Services | $194.7M | 13 |
| GENESIS REHABILITATION HOSPITAL INC | Jacksonville | E240 Health Care | $192.8M | 15 |
| UNIVERSITY ATHLETIC ASSOCIATION INC | Gainesville | N400 Recreation & Sports | $192.8M | 19 |
| Jacksonville University | Jacksonville | B430 Education | $190.9M | 8 |
| GIVEWELL COMMUNITY FOUNDATION INC | Lakeland | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $189.5M | 2 |
| VIERA HOSPITAL INC | Rockledge | E20 Health Care | $189.5M | 13 |
| EARLY LEARNING COALITION OF BROWARD | Fort Lauderdale | P12 Human Services | $187.8M | 5 |
| PINECREST ACADEMY INC | Miami | B29 Education | $184.9M | 21 |
| AAA SCHOLARSHIP FOUNDATION INC | Tampa | B21 Education | $180.4M | 1 |
| THE SPURLINO FOUNDATION | Odessa | — | $179.3M | 2 |
| GOODWILL INDUSTRIES OF SOUTH FLORIDA | Miami | J32Z Employment | $178.1M | 10 |
| SECOND HARVEST FOOD BANK OF | Orlando | — | $177M | 6 |
| Southeast Volusia Healthcare Corporation | New Smyrna Beach | N22 Recreation & Sports | $172M | 29 |
| MCR Health Inc | Bradenton | — | $171.7M | 16 |
| ADDITION FINANCIAL CREDIT UNION | Lake Mary | W61 Public & Societal Benefit | $170.5M | 12 |
| PENSACOLA CHRISTIAN COLLEGE INC | Pensacola | B200 Education | $169.8M | 8 |
| PEN AIR CREDIT UNION | Pensacola | — | $167.7M | 17 |
| Tarpon Springs Hospital Foundation Inc | Tarpon Springs | E220 Health Care | $165.4M | 26 |
| 26 HEALTH INC | Orlando | F70 Mental Health & Crisis Intervention | $164.4M | 9 |
| PRESBYTERIAN RETIREMENT COMMUNITIES | Orlando | P750 Human Services | $163.9M | 8 |
| GLOBAL EMPOWERMENT MISSION INC | Doral | M20 Public Safety, Disaster Preparedness & Relief | $162.9M | 1 |
| THE MOORINGS INCORPORATED | Naples | P750 Human Services | $162.2M | 9 |
