| PALM BEACH ATLANTIC UNIVERSITY INC | West Palm Beach | B420 Education | $161.4M | 18 |
| CHILDNET INC | Fort Lauderdale | P32 Human Services | $161.1M | 11 |
| International Generosity Foundation Trust | Clearwater | X12 Religion-Related | $160.3M | 0 |
| COMMUNITY FOUNDATION OF TAMPA BAY INC | Tampa | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $160M | 7 |
| C HERMAN & MARY VIRGINIA TERRY | Jacksonville | — | $159.6M | 0 |
| LIFELINK FOUNDATION INC | Tampa | E650 Health Care | $157.2M | 33 |
| FEEDING SOUTH FLORIDA INC | Pembroke Park | K31Z Food, Agriculture & Nutrition | $154.8M | 5 |
| CENTRO INTERNACIONAL DE AGRICULTURA TROPICAL | Medley | Q310 International, Foreign Affairs & National Security | $153.1M | 25 |
| BayCare Select Health Plans Inc | Clearwater | E31 Health Care | $151.6M | 5 |
| LYNN UNIVERSITY INC | Boca Raton | B430 Education | $150.4M | 13 |
| CHOCTAWHATCHEE ELECTRIC COOPERATIVE INC | Defuniak Springs | — | $149.2M | 16 |
| COMMUNITY FIRST CREDIT UNION OF FLORIDA | Jacksonville | — | $148.5M | 12 |
| GOODWILL INDUSTRIES - MANASOTA INC | Bradenton | J32Z Employment | $147M | 0 |
| TALQUIN ELECTRIC COOPERATIVE INC | Quincy | — | $146.9M | 17 |
| BROWARD REGIONAL HEALTH PLANNING COUNCIL | Hollywood | E00O Health Care | $145.8M | 3 |
| PEACE RIVER ELECTRIC COOPERATIVE | Wauchula | — | $144M | 15 |
| Eckerd College | St Petersburg | B420 Education | $143.9M | 14 |
| Florida Credit Union | Gainesville | — | $143.3M | 14 |
| Orlando Physicians Network Inc | Orlando | — | $142.8M | 12 |
| Baptist Health South Florida Foundation Inc | Coral Gables | E12 Health Care | $142.5M | 9 |
| SAINT LEO UNIVERSITY INC | Saint Leo | B43 Education | $141.7M | 9 |
| LIFESOUTH COMMUNITY BLOOD CENTERS | Gainesville | E61Z Health Care | $139.9M | 9 |
| STATE CHARTERED CREDIT UNIONS IN FLORIDA | Dunedin | — | $138.7M | 18 |
| THE MIAMI FOUNDATION INC | Miami | T30Z Philanthropy, Voluntarism & Grantmaking Foundations | $138.6M | 11 |
| THE DR M LEE PEARCE FOUNDATION INC | Miami Beach | — | $138.3M | 12 |
| RINGLING COLLEGE OF ART AND DESIGN INC | Sarasota | B420 Education | $137.5M | 8 |
| South Florida Behavioral Health Network Inc | Miami | F200 Mental Health & Crisis Intervention | $136.4M | 8 |
| SOUTHEASTERN UNIVERSITY INC | Lakeland | B420 Education | $135.5M | 15 |
| Christian & Missionary Alliance Foundation Inc | Fort Myers | P750 Human Services | $135.2M | 13 |
| CONFEDERATION OF NORTH CENTRAL AMERICAN AND | Miami | N64 Recreation & Sports | $135.1M | 27 |
| AMERICAN MARITIME OFFICERS VACATION PLAN | Dania Beach | — | $135.1M | 17 |
| NCHMD INC | Naples | E30 Health Care | $134.6M | 30 |
| H LEE MOFFITT CANCER CENTER & RESEARCH | Tampa | G30 Diseases, Disorders & Medical Disciplines | $134.3M | 17 |
| COMMUNITY HOSPICE OF NORTHEAST FLORIDA | Jacksonville | P74 Human Services | $131.9M | 9 |
| Lakeland Regional Health Systems Inc | Lakeland | E210 Health Care | $131.6M | 7 |
| PINE CREST PREPARATORY SCHOOL INC | Fort Lauderdale | B200 Education | $130.3M | 14 |
| EPISCOPAL CHILDREN'S SERVICES INC | Jacksonville | P330 Human Services | $129.4M | 8 |
| Barbara Newington Foundation | Palm Beach | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $128.7M | 4 |
| Lakeview Center Inc | Pensacola | F320 Mental Health & Crisis Intervention | $127.7M | 10 |
| AdventHealth Palm Coast Parkway Inc | Palm Coast | — | $126.3M | 12 |
| SUNRISE COMMUNITY INC | Miami | P820 Human Services | $122.1M | 9 |
| FLORIDA PACE CENTERS INC | Miami | L11 Housing & Shelter | $121.1M | 13 |
| CENTRAL FLORIDA CARES HEALTH SYSTEM INC | Orlando | P80 Human Services | $120.8M | 5 |
| Folx Foundation Inc | Miami Beach | — | $120.6M | 0 |
| Mariners Hospital Inc | Tavernier | E22 Health Care | $120.1M | 9 |
| CARE RESOURCE COMMUNITY HEALTH CENTERS | Miami | G81Z Diseases, Disorders & Medical Disciplines | $119.8M | 13 |
| FRANKLIN ACADEMY FOUNDATION INC | Fort Lauderdale | B29 Education | $117.7M | 6 |
| GOODWILL INDUSTRIES OF CENTRAL | Orlando | — | $117.3M | 8 |
| EARLY LEARNING COALITION OF DUVAL | Jacksonville | B012 Education | $114.8M | 5 |
| THE FLORIDA CHARTER EDUCATIONAL | Fort Lauderdale | B20 Education | $114.6M | 0 |
