| GLOBAL EMPOWERMENT MISSION INC | Doral | M20 Public Safety, Disaster Preparedness & Relief | $162.9M | 1 |
| CHOCTAWHATCHEE ELECTRIC COOPERATIVE INC | Defuniak Springs | — | $162.7M | 16 |
| MCR Health Inc | Bradenton | — | $162.6M | 18 |
| THE MOORINGS INCORPORATED | Naples | P750 Human Services | $162.2M | 9 |
| CHILDNET INC | Fort Lauderdale | P32 Human Services | $161.1M | 11 |
| JOHN AND DARIA BARRY FOUNDATION | Miami | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $159.1M | 0 |
| Eckerd College | St Petersburg | B420 Education | $158.9M | 11 |
| MUSEUM OF ARTS AND SCIENCES INC | Daytona Beach | A500 Arts, Culture & Humanities | $154M | 1 |
| HILLSBOROUGH COUNTY SCHOOL READINESS COALITION INC | Tampa | B012 Education | $153.9M | 4 |
| CENTRO INTERNACIONAL DE AGRICULTURA TROPICAL | Medley | Q310 International, Foreign Affairs & National Security | $153.1M | 25 |
| PENSACOLA CHRISTIAN COLLEGE INC | Pensacola | B200 Education | $151.9M | 8 |
| BayCare Select Health Plans Inc | Clearwater | E31 Health Care | $151.6M | 5 |
| LYNN UNIVERSITY INC | Boca Raton | B430 Education | $150.4M | 13 |
| ORANGE COUNTY SCHOOL READINESS COALITION INC | Orlando | B21 Education | $147.8M | 1 |
| CARE RESOURCE COMMUNITY HEALTH CENTERS INCORPORATED | Miami | G81Z Diseases, Disorders & Medical Disciplines | $147.4M | 14 |
| GOODWILL INDUSTRIES - MANASOTA INC | Bradenton | J32Z Employment | $147M | 12 |
| TALQUIN ELECTRIC COOPERATIVE INC | Quincy | — | $146.9M | 17 |
| BROWARD REGIONAL HEALTH PLANNING COUNCIL | Hollywood | E00O Health Care | $145.8M | 3 |
| H LEE MOFFITT CANCER CENTER & RESEARCH INST LIFETIME CANCER SCREENING CTR INC | Tampa | G30 Diseases, Disorders & Medical Disciplines | $145.7M | 25 |
| Chapters Health System Inc | Temple Terrace | P127 Human Services | $145.5M | 17 |
| PEACE RIVER ELECTRIC COOPERATIVE INC | Wauchula | — | $144M | 15 |
| Orlando Physicians Network Inc | Orlando | — | $142.8M | 12 |
| WTA TOUR INC | St Petersburg | — | $142.6M | 16 |
| Baptist Health South Florida Foundation Inc | Coral Gables | E12 Health Care | $142.5M | 9 |
| THE FLORIDA CHARTER EDUCATIONAL FOUNDATION INC | Ft Lauderdale | B20 Education | $142.5M | 0 |
| SAINT LEO UNIVERSITY INC | Saint Leo | B43 Education | $141.7M | 9 |
| PINE CREST PREPARATORY SCHOOL INC | Fort Lauderdale | B200 Education | $141.4M | 13 |
| JOHN S AND JAMES L KNIGHT FOUNDATION | Miami | B82 Education | $139.9M | 17 |
| LIFESOUTH COMMUNITY BLOOD CENTERS INC | Gainesville | E61Z Health Care | $139.9M | 9 |
| THE MIAMI FOUNDATION INC | Miami | T30Z Philanthropy, Voluntarism & Grantmaking Foundations | $138.6M | 11 |
| PALM BEACH ATLANTIC UNIVERSITY INC | West Palm Beach | B420 Education | $138.5M | 17 |
| THE DR M LEE PEARCE FOUNDATION INC | Miami Beach | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $138.3M | 12 |
| RINGLING COLLEGE OF ART AND DESIGN INC | Sarasota | B420 Education | $137.5M | 8 |
| South Florida Behavioral Health Network Inc | Miami | F200 Mental Health & Crisis Intervention | $136.4M | 8 |
| SOUTHEASTERN UNIVERSITY INC | Lakeland | B420 Education | $135.5M | 15 |
| Christian & Missionary Alliance Foundation Inc | Fort Myers | P750 Human Services | $135.2M | 13 |
| CONFEDERATION OF NORTH CENTRAL AMERICAN AND CARIBBEAN ASSOCIATION FOOTBALL (CONCACAF) | Miami | N64 Recreation & Sports | $135.1M | 27 |
| AMERICAN MARITIME OFFICERS VACATION PLAN | Dania Beach | — | $135.1M | 17 |
| NCHMD INC | Naples | E30 Health Care | $134.6M | 30 |
| INTERNATIONAL GENEROSITY FOUNDATION TRUST | Clearwater | X12 Religion-Related | $133.2M | 0 |
| COMMUNITY HOSPICE OF NORTHEAST FLORIDA | Jacksonville | P74 Human Services | $131.9M | 9 |
| THE SANDS FAMILY FOUNDATION INC C/O WILDSTAR PARTNERS LLC | Delray Beach | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $131.9M | 1 |
| Lakeland Regional Health Systems Inc | Lakeland | E210 Health Care | $131.6M | 7 |
| BETTY WOLD JOHNSON FOUNDATION | West Palm Beach | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $129.2M | 0 |
| Barbara Newington Foundation Agreement dated 12/17/14 | Palm Beach | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $128.7M | 4 |
| University of South Florida Foundation Inc | Tampa | B110 Education | $126.4M | 18 |
| AdventHealth Palm Coast Parkway Inc | Palm Coast | — | $126.3M | 12 |
| GOODWILL INDUSTRIES OF CENTRAL FLORIDA INC | Orlando | — | $122.9M | 9 |
| SUNRISE COMMUNITY INC | Miami | P820 Human Services | $122.1M | 9 |
| SOUTHEAST FLORIDA BEHAVIORAL HEALTH NETWORK INC | Jupiter | F30 Mental Health & Crisis Intervention | $121.8M | 3 |
