| BETHESDA HEALTHCARE INC | Cincinnati | E320 Health Care | $25.3M | 14 |
| MIDWEST COMMUNITY HEALTH ASSOCIATES | Bryan | — | $25.2M | 10 |
| COMMUNITY DIALYSIS CENTER | Shaker Heights | E300 Health Care | $25.2M | 4 |
| OHIO HIGH SCHOOL ATHLETIC ASSOCIATION | Columbus | N400 Recreation & Sports | $25.2M | 5 |
| SETON EDUCATION PARTNERS | Cincinnati | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $25.2M | 5 |
| AKRON ZOOLOGICAL PARK | Akron | D500 Animal-Related | $25.2M | 10 |
| The Progressive Insurance Foundation | Cleveland | T21 Philanthropy, Voluntarism & Grantmaking Foundations | $25.1M | 0 |
| LIFT A LIFE FOUNDATION INC | Mayfield Heights | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $25.1M | 0 |
| COUNTRY CLUB INC | Pepper Pike | — | $25.1M | 6 |
| BAYLEY SENIOR CARE | Cincinnati | P750 Human Services | $25.1M | 4 |
| TRINITY HOSPITAL TWIN CITY | Dennison | — | $25M | 13 |
| NCC SOLUTIONS INC | North Royalton | F33 Mental Health & Crisis Intervention | $25M | 13 |
| MURTIS TAYLOR HUMAN SERVICES SYSTEM | Cleveland | F30Z Mental Health & Crisis Intervention | $24.9M | 7 |
| COMMUNITY SUPPORT SERVICES INC | Akron | F320 Mental Health & Crisis Intervention | $24.9M | 7 |
| AXESS FAMILY SERVICES INC | Ravenna | E20Z Health Care | $24.8M | 11 |
| CHOICES BEHAVIORAL HEALTH CARE | Toledo | I11 Crime & Legal-Related | $24.8M | 2 |
| The Centers for Families and Children | Cleveland | P200 Human Services | $24.8M | 7 |
| AKRON SUMMIT COMMUNITY ACTION INC | Akron | — | $24.8M | 6 |
| THE MCGREGOR FOUNDATION | East Cleveland | — | $24.7M | 8 |
| StriveTogether Inc | Cincinnati | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $24.6M | 13 |
| THE MONTEFIORE HOME | Beachwood | E910 Health Care | $24.5M | 8 |
| CARING PARTNERS INTERNATIONAL INC | Franklin | Q300 International, Foreign Affairs & National Security | $24.5M | 2 |
| WESTERN RESERVE REVITALIZATION | Cleveland | L20 Housing & Shelter | $24.5M | 4 |
| YMCA of Central Stark County | Canton | P27Z Human Services | $24.4M | 3 |
| 1381 RIVER VALLEY CREDIT UNION INC | Miamisburg | — | $24.3M | 9 |
| UNITED WAY OF CENTRAL OHIO INC | Columbus | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $24.3M | 9 |
| ST LUKE'S HOSPITAL | Maumee | E220 Health Care | $24.2M | 19 |
| WASHINGTON ELECTRIC COOPERATIVE IN | Marietta | — | $24.2M | 13 |
| PSA 3 AGENCY ON AGING | Lima | — | $24.1M | 3 |
| West Side Deutscher Frauen Verein | Strongsville | E910 Health Care | $24M | 4 |
| Euclid Avenue Development Corporation | Cleveland | B19 Education | $24M | 3 |
| TRUTH FOR LIFE | Solon | X20 Religion-Related | $24M | 4 |
| BEECH ACRES | Cincinnati | P300 Human Services | $24M | 8 |
| HORIZON EDUCATION CENTERS | North Olmsted | — | $24M | 1 |
| WELLINGTON SCHOOL | Columbus | — | $23.8M | 7 |
| THE DYNAMIC CATHOLIC INSTITUTE | Cincinnati | X22 Religion-Related | $23.8M | 8 |
| CUYAHOGA VALLEY CHRISTIAN ACADEMY | Cuyahoga Falls | B25Z Education | $23.7M | 3 |
| UNISON BEHAVIORAL HEALTH GROUP INC | Toledo | E30Z Health Care | $23.7M | 8 |
| CINCINNATI MUSEUM ASSOCIATION | Cincinnati | A510 Arts, Culture & Humanities | $23.7M | 7 |
| SECOND HARVEST FOOD BANK OF THE | Youngstown | K31Z Food, Agriculture & Nutrition | $23.6M | 1 |
| FIRELANDS ELECTRIC COOPERATIVE INC | New London | — | $23.6M | 15 |
| LIFECARE ALLIANCE | Columbus | K30Z Food, Agriculture & Nutrition | $23.5M | 1 |
| CENTRAL OHIO HEALTH CARE CONSORTIUM | Granville | — | $23.5M | 0 |
| FRIENDSHIP VILLAGE OF COLUMBUS OHIO INC | Columbus | P750 Human Services | $23.4M | 6 |
| REUBEN MCMILLAN FREE LIBRARY ASSOCIATION | Youngstown | B700 Education | $23.3M | 3 |
| GREATER COLUMBUS CONVENTION AND VISITORS | Columbus | — | $23.3M | 7 |
| CEMS OF OHIO INC | Columbus | E62 Health Care | $23.3M | 8 |
| MCV HEALTH CARE FACILITIES INC | Cincinnati | E91Z Health Care | $23.2M | 6 |
| LUTHER HOME OF MERCY | Williston | P730 Human Services | $23.2M | 3 |
| IUPAT WELFARE FUND OF WESTERN PENNSYLVANIA | Canfield | Y43 Mutual & Membership Benefit | $23.1M | 0 |
