| THE FAB FOUNDATION | Boston | MA | $6.5M | 3 |
| PARTNERSHIP ON AI TO BENEFIT PEOPLE AND SOCIETY | San Francisco | CA | $6.5M | 7 |
| CHABOT SPACE & SCIENCE CENTER FOUNDATION | Oakland | CA | $6.4M | 8 |
| THE ENTOMOLOGICAL SOCIETY OF AMERICA | Annapolis | MD | $6.4M | 7 |
| HEALTH AND ENVIRONMENTAL SCIENCES INSTITUTE | Washington | DC | $6.3M | 8 |
| IMEC INC | San Francisco | CA | $6.3M | 1 |
| GIVE TO RESEARCH | Yelm | WA | $6.2M | 0 |
| AMERICAN VACUUM SOCIETY INCORPORATED | New York | NY | $6.2M | 8 |
| THE AMERICAN PHYTOPATHOLOGICAL SOCIETY | St Paul | MN | $6.2M | 3 |
| CARBON CONTAINMENT LAB INC | New Haven | CT | $6.2M | 4 |
| MARINE SCIENCE FOUNDATION INC | Stuart | FL | $6M | 5 |
| PROJECT CETI INC | New York | NY | $6M | 3 |
| IGEM FOUNDATION INC | Cambridge | MA | $6M | 1 |
| KANREN INC | Lawrence | KS | $6M | 3 |
| SIERRA INSTITUTE FOR COMMUNITY & ENVIRONMENT | Taylorsville | CA | $5.9M | 1 |
| PowerMyLearning Inc | New York | NY | $5.9M | 10 |
| IMEC USA NANOELECTRONICS DESIGN CENTER INC | Kissimmee | FL | $5.8M | 7 |
| ORGANIZATION FOR EDUCATIONAL TECHNOLOGY AND CURRICULUM | Lake Oswego | OR | $5.7M | 2 |
| ALCOR LIFE EXTENSION FOUNDATION | Scottsdale | AZ | $5.7M | 7 |
| AMAZON CONSERVATION TEAM | Falls Church | VA | $5.7M | 4 |
| Harbor Branch Oceanographic Institute Foundation Inc | Vero Beach | FL | $5.6M | 0 |
| NORTH CENTRAL SOYBEAN RESEARCH PROGRAM | Ankeny | IA | $5.6M | 0 |
| ASNT FOUNDATION | Columbus | OH | $5.6M | 2 |
| TECHNOVATION | Menlo Park | CA | $5.6M | 4 |
| IOP PUBLISHING INCORPORATED | Philadelphia | PA | $5.6M | 6 |
| STOCKHOLM ENVIRONMENT INSTITUTE US INC | Somerville | MA | $5.6M | 10 |
| MID ATLANTIC CLEAN HYDROGEN HUB INC | Dover | DE | $5.5M | 3 |
| DELTARES USA INC | Silver Spring | MD | $5.5M | 1 |
| ELECTRI INTERNATIONAL - THE FOUNDATION FOR ELECTRICAL CONSTRUCTION INC | Washington | DC | $5.5M | 2 |
| THE PITTSBURGH CONFERENCE ON ANALYTICAL CHEMISTRY AND APPLIED SPECTROSCOPY | Monroeville | PA | $5.4M | 0 |
| Marine Technology Society Inc | Washington | DC | $5.4M | 2 |
| OpenAI Inc | San Francisco | CA | $5.4M | 5 |
| INSTITUTE OF NOETIC SCIENCES | Novato | CA | $5.4M | 4 |
| NORTHEASTERN REGIONAL ASSOCIATION OF COASTAL OCEAN OBSERVING SYSTEMS | Portsmouth | NH | $5.4M | 3 |
| THE EXPLORERS CLUB | New York | NY | $5.4M | 2 |
| TECHNOLOGY MATTERS | Palo Alto | CA | $5.3M | 9 |
| MASSACHUSETTS BIOMEDICAL INITIATIVES INC | Worcester | MA | $5.3M | 2 |
| CREIGHTON UNIVERSITY - BOYS TOWN HEALTHCARE FOUNDATION | Omaha | NE | $5.3M | 3 |
| CONSORTIUM OF UNIVERSITIES FOR THE ADVANCEMENT OF HYDROLOGIC SCIENCE INC | Arlington | MA | $5.3M | 7 |
| SEQUOIA FARM FOUNDATION | Decatur | IL | $5.2M | 0 |
| MOLLUSCAN SCIENCE FOUNDATION | Owings Mills | MD | $5.2M | 1 |
| POST ROAD FOUNDATION | Oakland | CA | $5.2M | 1 |
| APPRISE Incorporated | Princeton | NJ | $5.1M | 3 |
| SCIENCE AND TECHNOLOGY FUTURES INC | Cambridge | MA | $5.1M | 5 |
| Association for the Advancement of Artificial Inte | Washington | DC | $5.1M | 2 |
| POPULATION BIOLOGY FOUNDATION | Grand Junction | CO | $5.1M | 0 |
| AMERICAN OIL CHEMISTS' SOCIETY | Champaign | IL | $5M | 6 |
| MIND RESEARCH INSTITUTE INC | Irvine | CA | $4.9M | 3 |
| INSTITUTE FOR SECURITY AND TECHNOLOGY | Oakland | CA | $4.8M | 2 |
| BLUE MARBLE SPACE | Seattle | WA | $4.8M | 5 |
