| LUTHERAN SOCIAL SERVICES OF SOUTH DAKOTA | Sioux Falls | — | $29.5M | 2 |
| DAKOTA WESLEYAN UNIVERSITY | Mitchell | B420 Education | $29.3M | 3 |
| WINNER REGIONAL HEALTHCARE CENTER | Winner | E22 Health Care | $28.4M | 7 |
| DAKOTA ENERGY COOPERATIVE INC | Huron | — | $28.3M | 16 |
| SDSM&T CENTER FOR ALUMNI RELATIONS | Rapid City | B5XZ Education | $28M | 4 |
| MID-DAKOTA RURAL WATER SYSTEM INC | Miller | — | $27.9M | 9 |
| BLACK HILLS ELECTRIC COOPERATIVE INC | Custer | — | $25.8M | 16 |
| LEWIS AND CLARK BEHAVIORAL | Yankton | F320 Mental Health & Crisis Intervention | $24.8M | 4 |
| DAKOTABILITIES INC | Sioux Falls | P820 Human Services | $23.7M | 3 |
| COTEAU DES PRAIRIES HOSPITAL | Sisseton | E20 Health Care | $22.2M | 5 |
| SOUTHEASTERN BEHAVIORAL HEALTH CARE | Sioux Falls | F300 Mental Health & Crisis Intervention | $21.8M | 7 |
| SIOUX FALLS DEVELOPMENT | Sioux Falls | — | $21.6M | 4 |
| PLATTE COMMUNITY MEMORIAL HOSPITAL INC | Platte | E20Z Health Care | $21.2M | 7 |
| LAKE REGION ELECTRIC ASSOCIATION INC | Webster | — | $21.2M | 13 |
| SOUTH DAKOTA BANKERS BENEFIT PLAN TRUST | Pierre | Y43 Mutual & Membership Benefit | $21.1M | 0 |
| FREEMAN REGIONAL HEALTH SERVICES | Freeman | E22Z Health Care | $20.4M | 3 |
| LIFEQUEST INC | Mitchell | P820 Human Services | $19.9M | 0 |
| PHILIP HEALTH SERVICES INC | Philip | E21Z Health Care | $19.8M | 5 |
| LITTLE WOUND SCHOOL BOARD INC | Kyle | B200 Education | $19.7M | 13 |
| WEB WATER DEVELOPMENT ASSOCIATION | Aberdeen | — | $19.4M | 14 |
| SICANGU OYATE HO INC | Saint Francis | B20 Education | $19M | 11 |
| WEST CENTRAL ELECTRIC COOPERATIVE | Murdo | — | $18.5M | 16 |
| BETHANY LUTHERAN HOME FOR THE AGED INC | Sioux Falls | E910 Health Care | $18.5M | 3 |
| FEM ELECTRIC ASSOCIATION INC | Ipswich | Z99 Unknown | $18.2M | 10 |
| HURON AREA CENTER FOR INDEPENDENCE | Huron | J300 Employment | $17.6M | 0 |
| SANFORD | Sioux Falls | E21 Health Care | $17.4M | 5 |
| HUMAN SERVICE AGENCY | Watertown | P820 Human Services | $17.4M | 3 |
| SIOUX FALLS CHRISTIAN SCHOOLS | Sioux Falls | B11 Education | $17.1M | 2 |
| INTER-LAKES COMMUNITY ACTION | Madison | P200 Human Services | $17M | 3 |
| CHERRY TODD ELECTRIC COOPERATIVE INC | Mission | — | $16.6M | 15 |
| LACREEK ELECTRIC ASSOCIATION INC | Martin | — | $16.5M | 17 |
| PIONEER MEMORIAL HOSPITAL AND | Viborg | E20 Health Care | $16.4M | 6 |
| CODINGTON-CLARK ELECTRIC COOPERATIVE | Watertown | — | $16.4M | 13 |
| Presentation College | Aberdeen | B42Z Education | $16.1M | 4 |
| F-M AMBULANCE SERVICE INC | Sioux Falls | E60 Health Care | $16M | 5 |
| ELEVATE RAPID CITY | Rapid City | — | $15.9M | 1 |
| RURAL OFFICE OF COMMUNITY SERVICES | Wagner | — | $15.9M | 0 |
| YOUTH & FAMILY SERVICES INC | Rapid City | — | $15.6M | 5 |
| H-D ELECTRIC COOPERATIVE INC | Clear Lake | — | $15.3M | 17 |
| ST MICHAEL'S HOSPITAL INC | Tyndall | — | $15M | 7 |
| WAGNER COMMUNITY MEMORIAL HOSPITAL | Wagner | E22Z Health Care | $14.7M | 7 |
| CEDAR LAKE VILLAGE INC | Sioux Falls | L22 Housing & Shelter | $14.7M | 0 |
| WASHINGTON PAVILION MANAGEMENT INC | Sioux Falls | A200 Arts, Culture & Humanities | $14.6M | 4 |
| JENKINS LIVING CENTER INC | Watertown | P750 Human Services | $14.3M | 1 |
| Bethesda Home of Aberdeen Inc | Aberdeen | — | $14.1M | 2 |
| BLACK HILLS AREA COMMUNITY | Rapid City | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $13.9M | 1 |
| GRAND ELECTRIC COOPERATIVE INC | Bison | — | $13.8M | 17 |
| MARSHALL COUNTY HEALTHCARE CENTER | Britton | E22Z Health Care | $13.6M | 5 |
| CRAZY HORSE MEMORIAL FOUNDATION | Crazy Horse | A85Z Arts, Culture & Humanities | $13.6M | 8 |
| Northern State University Foundation | Aberdeen | — | $13.5M | 3 |
