| FLORIDA PACE CENTERS INC | Miami | L11 Housing & Shelter | $121.1M | 13 |
| CENTRAL FLORIDA CARES HEALTH SYSTEM INC | Orlando | P80 Human Services | $120.8M | 5 |
| Folx Foundation Inc | Miami Beach | E60 Health Care | $120.6M | 0 |
| Mariners Hospital Inc | Tavernier | E22 Health Care | $120.1M | 9 |
| Global Connections to Employment Inc | Pensacola | P80 Human Services | $119.2M | 10 |
| FRANKLIN ACADEMY FOUNDATION INC | Fort Lauderdale | B29 Education | $117.7M | 6 |
| GULLIVER PREPARATORY SCHOOL INC | Pinecrest | B200 Education | $117.1M | 9 |
| Lakeview Center Inc | Pensacola | F320 Mental Health & Crisis Intervention | $114.9M | 11 |
| EARLY LEARNING COALITION OF DUVAL INC | Jacksonville | B012 Education | $114.8M | 5 |
| ORLANDOORANGE COUNTY CONVENTION & VISITORS BUREAU INC | Orlando | — | $114.4M | 12 |
| BRIDGEPREP ACADEMY INC | Miami | B24 Education | $114M | 6 |
| ABEKA ACADEMY INC | Pensacola | B90Z Education | $113.6M | 1 |
| TRIUMPH GULF COAST INC | Tallahassee | P20 Human Services | $113M | 1 |
| MIAMI CANCER INSTITUTE AT BAPTIST HEALTH INC | Coral Gables | G30 Diseases, Disorders & Medical Disciplines | $112.9M | 15 |
| HOPE HOSPICE AND COMMUNITY SERVICES INC | Temple Terrace | P74Z Human Services | $112.8M | 18 |
| DEFENSEWERX INC | Niceville | U40 Science & Technology | $112.5M | 1 |
| AdventHealth Polk South Inc | Lake Wales | — | $111.4M | 12 |
| TIDEWELL HOSPICE INC | Lakewood Ranch | P740 Human Services | $111.4M | 10 |
| iTHINK Financial Credit Union | Delray Beach | W61 Public & Societal Benefit | $109.4M | 18 |
| THE POSES FAMILY FOUNDATION | Miami Beach | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $109.3M | 0 |
| The Florida State University Foundation Inc | Tallahassee | B114 Education | $109.1M | 13 |
| ASPIRE HEALTH PARTNERS INC | Orlando | F31 Mental Health & Crisis Intervention | $108.9M | 14 |
| INDEPENDENT COLLEGES & UNIVERSITIES BENEFITS ASSOCIATION INC | Orlando | — | $108.7M | 11 |
| REDLANDS CHRISTIAN MIGRANT ASSOCIATION INC | Immokalee | P84Z Human Services | $108.5M | 9 |
| THE HOSPICE OF THE FLORIDA SUNCOAST INC | Lakewood Ranch | P740 Human Services | $107.6M | 9 |
| MORSELIFE HOME CARE INC | West Palm Beach | E92Z Health Care | $107.4M | 9 |
| FAMILY HEALTH CENTERS OF SOUTHWEST | Fort Myers | E320 Health Care | $107M | 14 |
| UCF Athletics Association Inc | Orlando | B19 Education | $106.7M | 8 |
| HEALTH FIRST COMMERCIAL PLANS INC | Rockledge | — | $106.7M | 10 |
| FLAGLER COLLEGE INC | St Augustine | B420 Education | $106.6M | 12 |
| FLORIDA TOURISM INDUSTRY MARKETING CORP INC | Tallahassee | — | $105.9M | 8 |
| MIGHTYCAUSE CHARITABLE FOUNDATION | Marianna | T50 Philanthropy, Voluntarism & Grantmaking Foundations | $105.9M | 1 |
| FLORIDA KEYS ELECTRIC COOPERATIVE ASSOCIATION INC | Tavernier | Y22 Mutual & Membership Benefit | $105.8M | 17 |
| BROWARD BEHAVIORAL HEALTH COALITION INC | Lauderhill | F99 Mental Health & Crisis Intervention | $105.4M | 3 |
| AAA SCHOLARSHIP FOUNDATION INC | Tampa | B21 Education | $104.4M | 1 |
| THE COMMUNITY FOUNDATION FOR NORTHEAST FLORIDA INC | Jacksonville | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $104.3M | 6 |
| Bartow Regional Medical Center Inc | Bartow | E20 Health Care | $103.7M | 10 |
| MIDWAY SPECIALTY CARE CENTER INC | Fort Pierce | E32 Health Care | $103.3M | 12 |
| Baptist Medical Center of Nassau Inc | Jacksonville | E220 Health Care | $102.8M | 11 |
| CENTRAL FLORIDA HEALTH CARE INC | Winter Haven | E39Z Health Care | $102.6M | 15 |
| Greater Miami Jewish Federation Inc | Miami | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $102.3M | 13 |
| GOODWILL INDUSTRIES - SUNCOAST INC | St Petersburg | J327 Employment | $100.2M | 13 |
| BETHUNE-COOKMAN UNIVERSITY | Daytona Beach | B420 Education | $100.2M | 11 |
| SOUTHWEST CHARTER FOUNDATION INC | Fort Lauderdale | B29 Education | $99.8M | 1 |
| Episcopal Children's Services Inc | Jacksonville | P330 Human Services | $99.8M | 9 |
| THE DORAL ACADEMY INC | Miami | B29 Education | $96.5M | 7 |
| FLORIDIANS PROTECTING FREEDOM | Miami | — | $95.7M | 3 |
| Florida Hospital Dade City Inc | Dade City | — | $94.9M | 15 |
| Cornerstone Hospice and Palliative Care Inc | Temple Terrace | P75Z Human Services | $94.1M | 17 |
| COMMUNITY COORDINATED CARE FOR CHILDREN INC | Orlando | P33Z Human Services | $93.7M | 4 |
