| ORLANDOORANGE COUNTY CONVENTION | Orlando | — | $114.4M | 12 |
| ABEKA ACADEMY INC | Pensacola | B90Z Education | $113.6M | 1 |
| GULLIVER PREPARATORY SCHOOL INC | Pinecrest | B200 Education | $113.5M | 8 |
| RANSOM EVERGLADES SCHOOL INC | Miami | B200 Education | $113.3M | 10 |
| TRIUMPH GULF COAST INC | Tallahassee | P20 Human Services | $113M | 1 |
| MIAMI CANCER INSTITUTE AT BAPTIST HEALTH INC | Coral Gables | G30 Diseases, Disorders & Medical Disciplines | $112.9M | 15 |
| HOPE HOSPICE AND COMMUNITY SERVICES INC | Temple Terrace | P74Z Human Services | $112.8M | 18 |
| DEFENSEWERX INC | Niceville | U40 Science & Technology | $112.5M | 1 |
| TIDEWELL HOSPICE INC | Lakewood Ranch | P740 Human Services | $111.4M | 10 |
| THE SANDS FAMILY FOUNDATION INC | Delray Beach | — | $109.5M | 1 |
| iTHINK Financial Credit Union | Delray Beach | W61 Public & Societal Benefit | $109.4M | 18 |
| THE POSES FAMILY FOUNDATION | Miami Beach | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $109.3M | 0 |
| ASPIRE HEALTH PARTNERS INC | Orlando | F31 Mental Health & Crisis Intervention | $109.2M | 12 |
| INDEPENDENT COLLEGES & UNIVERSITIES | Orlando | — | $108.7M | 11 |
| THE HOSPICE OF THE FLORIDA SUNCOAST INC | Lakewood Ranch | P740 Human Services | $107.6M | 9 |
| MORSELIFE HOME CARE INC | West Palm Beach | E92Z Health Care | $107.4M | 9 |
| Global Connections to Employment Inc | Pensacola | P80 Human Services | $107.2M | 9 |
| FAMILY HEALTH CENTERS OF SOUTHWEST | Fort Myers | E320 Health Care | $107M | 14 |
| HEALTH FIRST COMMERCIAL PLANS INC | Rockledge | — | $106.7M | 10 |
| FLORIDA TOURISM INDUSTRY MARKETING | Tallahassee | — | $105.9M | 8 |
| FLORIDA KEYS ELECTRIC COOPERATIVE | Tavernier | Y22 Mutual & Membership Benefit | $105.8M | 17 |
| BROWARD BEHAVIORAL HEALTH COALITION INC | Lauderhill | F99 Mental Health & Crisis Intervention | $105.4M | 3 |
| Chapters Health System Inc | Temple Terrace | P127 Human Services | $105.2M | 18 |
| JOHN AND DARIA BARRY FOUNDATION | Miami | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $105M | 0 |
| THE COMMUNITY FOUNDATION FOR | Jacksonville | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $104.3M | 6 |
| MARGUERITE DELANY HARK FOUNDATION | Venice | — | $103.9M | 3 |
| Bartow Regional Medical Center Inc | Bartow | E20 Health Care | $103.7M | 10 |
| MIDWAY SPECIALTY CARE CENTER INC | Fort Pierce | E32 Health Care | $103.3M | 12 |
| Baptist Medical Center of Nassau Inc | Jacksonville | E220 Health Care | $102.8M | 11 |
| SOUTHEAST FLORIDA BEHAVIORAL HEALTH | Jupiter | F30 Mental Health & Crisis Intervention | $102.6M | 3 |
| BETHUNE-COOKMAN UNIVERSITY | Daytona Beach | B420 Education | $102.2M | 12 |
| REDLANDS CHRISTIAN MIGRANT | Immokalee | P84Z Human Services | $98.2M | 7 |
| THE WARREN AND AUGUSTA HUME FOUNDATION | Ponte Vedra Beach | — | $97.7M | 7 |
| AdventHealth Polk South Inc | Lake Wales | — | $97M | 16 |
| THE DORAL ACADEMY INC | Miami | B29 Education | $96.5M | 7 |
| FLAGLER COLLEGE INC | St Augustine | B420 Education | $96.1M | 13 |
| THE FLORIDA STATE UNIVERSITY FOUNDATION | Tallahassee | B114 Education | $96M | 15 |
| Greater Miami Jewish Federation Inc | Miami | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $95.7M | 13 |
| Florida Hospital Dade City Inc | Dade City | — | $94.9M | 15 |
| Cornerstone Hospice and Palliative Care Inc | Temple Terrace | P75Z Human Services | $94.1M | 17 |
| UNIVERSITY OF CENTRAL FLORIDA | Orlando | B110 Education | $94.1M | 10 |
| COMMUNITY COORDINATED CARE FOR | Orlando | P33Z Human Services | $93.7M | 4 |
| SMA HEALTHCARE INC | Daytona Beach | F320 Mental Health & Crisis Intervention | $93.6M | 12 |
| GOODWILL INDUSTRIES - SUNCOAST INC | St Petersburg | J327 Employment | $93.1M | 12 |
| THE MARIE-JOSEE AND HENRY R KRAVIS | West Palm Beach | — | $92.9M | 0 |
| HEALTH FIRST INC | Rockledge | — | $92M | 0 |
| Capital Hospice | Temple Terrace | P74 Human Services | $91.9M | 21 |
| POLK COUNTY SCHOOL READINESS COALITION | Lakeland | B012 Education | $91.2M | 4 |
| Fishermens Health Inc | Marathon | E22 Health Care | $89.5M | 9 |
| SEMINOLE BOOSTERS INC | Tallahassee | B110 Education | $88.8M | 11 |
