| JOHN S AND JAMES L KNIGHT FOUNDATION | Miami | B82 Education | $86.9M | 20 |
| MIGHTYCAUSE CHARITABLE FOUNDATION | Marianna | T50 Philanthropy, Voluntarism & Grantmaking Foundations | $86.3M | 1 |
| St Thomas University Inc | Miami | — | $86M | 11 |
| SOUTHWEST CHARTER FOUNDATION INC | Fort Lauderdale | B29 Education | $85.5M | 0 |
| THE SHRINERS' HOSPITAL FOR CHILDREN | Tampa | E240 Health Care | $84.9M | 6 |
| BOCA WEST COUNTRY CLUB INC | Boca Raton | — | $84.2M | 7 |
| Community Health of South Florida Inc | Miami | E32Z Health Care | $83.6M | 7 |
| MOUNT SINAI MEDICAL CENTER FOUNDATION | Miami Beach | E110 Health Care | $83.4M | 6 |
| MIAMI JEWISH HEALTH SYSTEMS INC | Miami | P750 Human Services | $83.3M | 15 |
| CENTRAL FLORIDA HEALTH CARE INC | Winter Haven | E39Z Health Care | $83.2M | 15 |
| THE CHILDREN'S HOME SOCIETY OF FLORIDA | Orlando | P300 Human Services | $82.6M | 12 |
| JOHN KNOX VILLAGE OF FLORIDA INC | Pompano Beach | P750 Human Services | $82.3M | 9 |
| COMMUNITY HEALTH CENTERS INC | Winter Garden | — | $82.1M | 13 |
| KIDS CENTRAL INC | Wildwood | E60 Health Care | $82M | 7 |
| HARRY CHAPIN FOOD BANK OF SOUTHWEST | Fort Myers | K31Z Food, Agriculture & Nutrition | $81.5M | 3 |
| THE COMMUNITY FOUNDATION OF SARASOTA CO | Sarasota | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $80.7M | 9 |
| LifePath Hospice Inc | Temple Terrace | E92 Health Care | $80.6M | 18 |
| MIAMI COUNTRY DAY SCHOOL INC | Miami | B200 Education | $80.4M | 12 |
| MOTE MARINE LABORATORY INC | Sarasota | — | $80M | 7 |
| UCF Athletics Association Inc | Orlando | B19 Education | $79.3M | 8 |
| TAMPA GENERAL MEDICAL GROUP INC | Tampa | E60 Health Care | $78.7M | 13 |
| TAMPA FAMILY HEALTH CENTERS INC | Tampa | E32Z Health Care | $78.6M | 11 |
| WEST FLORIDA ELECTRIC COOPERATIVE | Graceville | — | $78.5M | 22 |
| Morton Plant Mease Health Services Inc | Clearwater | E21 Health Care | $78.1M | 10 |
| LAUNCH CREDIT UNION | Merritt Island | — | $78.1M | 10 |
| TRENTON MEDICAL CENTER INC | High Springs | G30Z Diseases, Disorders & Medical Disciplines | $77.3M | 10 |
| THE FREDERICK A DELUCA FOUNDATION INC | Pompano Beach | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $77.3M | 0 |
| TAMPA GENERAL PROVIDER NETWORK INC | Tampa | E30 Health Care | $77.1M | 9 |
| Miami Beach Community Health Center Inc | North Miami | E20Z Health Care | $76.7M | 11 |
| THE EARLY LEARNING COALITION OF FLAGLER | Daytona Beach | B192 Education | $76.2M | 4 |
| GULF COAST ELECTRIC COOPERATIVE | Wewahitchka | Y22 Mutual & Membership Benefit | $76.1M | 16 |
| EMBRACE FAMILIES COMMUNITY BASED | Maitland | P40 Human Services | $76.1M | 4 |
| ANA G MENDEZ UNIVERSITY INC | Orlando | B40 Education | $75.7M | 12 |
| THE BOLLES SCHOOL | Jacksonville | B200 Education | $75.4M | 7 |
| COMMUNITY HEALTH CENTERS OF PINELLAS INC | Clearwater | E32Z Health Care | $75.3M | 11 |
| CENTRAL FLORIDA ELECTRIC | Chiefland | — | $75.1M | 20 |
| EARLY LEARNING COALITION OF | Clearwater | P33 Human Services | $74.9M | 2 |
| ADVANTAGE ACADEMY OF HILLSBOROUGH INC | Coral Springs | B29 Education | $74.6M | 6 |
| IDEA FLORIDA INC | Tampa | B29 Education | $74.3M | 7 |
| Regional Food Bank of Northeast Florida Inc | Jacksonville | K31 Food, Agriculture & Nutrition | $74.2M | 6 |
| THE EARLY LEARNING COALITION OF | Fort Myers | B012 Education | $74M | 6 |
| THE INSTITUTE OF INTERNAL AUDITORS INC | Lake Mary | — | $73.4M | 13 |
| THE LAURA & ISAAC PERLMUTTER FOUNDATION | Lake Worth | T90Z Philanthropy, Voluntarism & Grantmaking Foundations | $73.1M | 1 |
| AVE MARIA UNIVERSITY INC | Ave Maria | B43 Education | $72.6M | 19 |
| CATHOLIC HOSPICE INC | Miami Lakes | P74Z Human Services | $71.4M | 12 |
| LAKE HIGHLAND PREPARATORY SCHOOL INC | Orlando | B25Z Education | $71.4M | 11 |
| Hospice of Palm Beach County Inc | Lakewood Ranch | P74 Human Services | $70.3M | 0 |
| LIFESTREAM BEHAVIORAL CENTER INC | Leesburg | E24 Health Care | $70.1M | 9 |
| Suwannee Valley Electric Cooperative | Live Oak | — | $70M | 18 |
| GIVE KIDS THE WORLD INC | Kissimmee | — | $69.6M | 9 |
