| SMA HEALTHCARE INC | Daytona Beach | F320 Mental Health & Crisis Intervention | $93.6M | 12 |
| RANSOM EVERGLADES SCHOOL INC | Miami | B200 Education | $91M | 8 |
| COVENANT HEALTH & COMMUNITY SERVICES INC | Pensacola | E19 Health Care | $90.9M | 7 |
| Capital Hospice | Temple Terrace | P74 Human Services | $90.2M | 20 |
| Fishermens Health Inc | Marathon | E22 Health Care | $89.5M | 9 |
| ST THOMAS UNIVERSITY INC | Miami | — | $88.8M | 11 |
| MIAMI JEWISH HEALTH SYSTEMS INC | Miami | P750 Human Services | $88M | 15 |
| JOHN KNOX VILLAGE OF FLORIDA INC | Pompano Beach | P750 Human Services | $86.5M | 10 |
| LAUNCH CREDIT UNION | Merritt Island | — | $85.2M | 10 |
| THE SHRINERS' HOSPITAL FOR CHILDREN | Tampa | E240 Health Care | $84.9M | 6 |
| THE CHILDREN'S HOME SOCIETY OF FLORIDA | Orlando | P300 Human Services | $84.5M | 13 |
| BOCA WEST COUNTRY CLUB INC | Boca Raton | — | $84.2M | 7 |
| Community Health of South Florida Inc | Miami | E32Z Health Care | $83.6M | 7 |
| MOUNT SINAI MEDICAL CENTER FOUNDATION | Miami Beach | E110 Health Care | $83.4M | 6 |
| LifePath Hospice Inc | Temple Terrace | E92 Health Care | $82.1M | 16 |
| COMMUNITY HEALTH CENTERS INC | Winter Garden | — | $82.1M | 13 |
| THE INSTITUTE OF INTERNAL AUDITORS INC | Lake Mary | — | $81.8M | 20 |
| HARRY CHAPIN FOOD BANK OF SOUTHWEST FLORIDA INC | Fort Myers | K31Z Food, Agriculture & Nutrition | $81.5M | 3 |
| AVE MARIA UNIVERSITY INC | Ave Maria | B43 Education | $81.5M | 17 |
| KIDS CENTRAL INC | Wildwood | E60 Health Care | $81.2M | 7 |
| HEALTH FIRST INC EMPLOYEE BENEFIT TRUST | Rockledge | — | $80.7M | 0 |
| THE COMMUNITY FOUNDATION OF SARASOTA CO INC | Sarasota | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $80.7M | 9 |
| SEMINOLE BOOSTERS INC | Tallahassee | B110 Education | $80.6M | 15 |
| MIAMI COUNTRY DAY SCHOOL INC | Miami | B200 Education | $80.4M | 12 |
| MOTE MARINE LABORATORY INC | Sarasota | — | $80M | 7 |
| Morton Plant Mease Health Services Inc | Clearwater | E21 Health Care | $79.6M | 10 |
| TAMPA GENERAL MEDICAL GROUP INC | Tampa | E60 Health Care | $78.7M | 13 |
| TAMPA FAMILY HEALTH CENTERS INC | Tampa | E32Z Health Care | $78.6M | 11 |
| WEST FLORIDA ELECTRIC COOPERATIVE ASSOCIATION INC | Graceville | — | $78.5M | 22 |
| LENNAR FOUNDATION | Miami | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $78.3M | 1 |
| Emergency Assistance Foundation Inc | West Palm Beach | P60 Human Services | $78.1M | 1 |
| Suwannee Valley Electric Cooperative | Live Oak | — | $77.9M | 18 |
| Florida Radiology Imaging at Lake Mary LLC | Maitland | — | $77.9M | 9 |
| TRENTON MEDICAL CENTER INC | High Springs | G30Z Diseases, Disorders & Medical Disciplines | $77.3M | 10 |
| THE FREDERICK A DELUCA FOUNDATION INC | Pompano Beach | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $77.3M | 0 |
| TAMPA GENERAL PROVIDER NETWORK INC | Tampa | E30 Health Care | $77.1M | 9 |
| GULF WINDS CREDIT UNION | Pensacola | W61 Public & Societal Benefit | $77M | 11 |
| Miami Beach Community Health Center Inc | North Miami | E20Z Health Care | $76.7M | 11 |
| GULF COAST ELECTRIC COOPERATIVE | Wewahitchka | Y22 Mutual & Membership Benefit | $76.1M | 16 |
| EMBRACE FAMILIES COMMUNITY BASED CARE INC | Maitland | P40 Human Services | $76.1M | 4 |
| ANA G MENDEZ UNIVERSITY INC | Orlando | B40 Education | $75.7M | 12 |
| THE BOLLES SCHOOL C/O TYLER J HODGES | Jacksonville | B200 Education | $75.4M | 7 |
| COMMUNITY HEALTH CENTERS OF PINELLAS INC D/B/A EVARA HEALTH | Clearwater | E32Z Health Care | $75.3M | 11 |
| RESTORATION OF AMERICA | Ponte Vedra Beach | R11 Civil Rights, Social Action & Advocacy | $75.1M | 14 |
| CENTRAL FLORIDA ELECTRIC COOPERATIVE INC | Chiefland | — | $75.1M | 20 |
| EARLY LEARNING COALITION OF PINELLAS COUNTY INC | Clearwater | P33 Human Services | $74.9M | 2 |
| UNIVERSITY OF CENTRAL FLORIDA FOUNDATION INC | Orlando | B110 Education | $74.5M | 9 |
| IDEA FLORIDA INC | Tampa | B29 Education | $74.3M | 7 |
| Regional Food Bank of Northeast Florida Inc | Jacksonville | K31 Food, Agriculture & Nutrition | $74.2M | 6 |
| THE EARLY LEARNING COALITION OF SOUTHWEST FLORIDA INC | Fort Myers | B012 Education | $74M | 6 |
