| WELLSPIRE LLC | Johnston | — | $31.6M | 5 |
| VISITING NURSE SERVICES OF IOWA | Des Moines | E92Z Health Care | $31.4M | 10 |
| HEARTLAND POWER COOPERATIVE | Thompson | — | $31.3M | 12 |
| One Vision | Clear Lake | L200 Housing & Shelter | $31M | 6 |
| PDS VEBA TRUST | Des Moines | Y30 Mutual & Membership Benefit | $30.4M | 0 |
| RIVER HILLS COMMUNITY HEALTH CENTER | Ottumwa | E22 Health Care | $30.4M | 8 |
| IOWA LABORERS HEALTH & WELFARE FUND | Des Moines | — | $30.4M | 0 |
| IOWA NATURAL HERITAGE FOUNDATION | Des Moines | C300 Environment | $29.8M | 3 |
| UNITYPOINT RURAL HEALTH ALLIANCE | West Des Moines | Y43 Mutual & Membership Benefit | $29.6M | 1 |
| COMMUNITY HEALTH CENTERS OF SOUTHEASTERN IOWA INC | West Burlington | E32 Health Care | $28.9M | 9 |
| Avera Holy Family | Estherville | E220 Health Care | $28.5M | 7 |
| MID-STEP SERVICES INC | Sioux City | P700 Human Services | $28.4M | 3 |
| Iowa Wesleyan University | Mount Pleasant | — | $28.4M | 3 |
| IOWA CORN GROWER'S ASSOCIATION | Johnston | — | $28.3M | 23 |
| HILLS AND DALES CHILD DEVELOPMENT CENTER | Dubuque | E91Z Health Care | $28M | 5 |
| SIOUXLAND PACE INC | Sioux City | E99 Health Care | $28M | 8 |
| Stonehill Franciscan Services | Dubuque | P750 Human Services | $27.9M | 6 |
| IDA COUNTY IOWA COMMUNITY HOSPITAL INC | Ida Grove | E22 Health Care | $27.8M | 8 |
| GREATER DES MOINES HABITAT FOR HUMANITY | Des Moines | L20Z Housing & Shelter | $27.8M | 6 |
| OKLAHOMA GAS AND ELECTRIC COMPANY | Des Moines | — | $27.6M | 1 |
| OPERATING ENGINEERS LOCAL #234 HEALTH | Des Moines | — | $27.4M | 0 |
| EAST-CENTRAL IOWA RURAL ELECTRIC | Urbana | — | $27M | 14 |
| University of Iowa Strategic Initiatives Fund | Iowa City | B11 Education | $26.8M | 4 |
| SHELL OIL DENTAL ASSISTANCE PLAN | Des Moines | — | $26.6M | 1 |
| WOODWARD YOUTH CORPORATION | Woodward | Z99Z Unknown | $26.6M | 9 |
| FRIENDSHIP HAVEN INC | Fort Dodge | L20 Housing & Shelter | $26.3M | 7 |
| CHRISTIAN RETIREMENT SERVICES INC | Iowa City | P750 Human Services | $26.3M | 5 |
| HEGG MEMORIAL HOSPITAL | Rock Valley | E22Z Health Care | $26.2M | 5 |
| DES MOINES PERFORMING ARTS | Des Moines | S200 Community Improvement & Capacity Building | $26.2M | 7 |
| FRIENDS OF FAITH RET HOMES INC | Waterloo | L220 Housing & Shelter | $26.2M | 2 |
| IOWA WEST FOUNDATION | Council Bluffs | — | $25.9M | 1 |
| MidAmerican Energy Foundation | Des Moines | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $25.8M | 0 |
| UNITED WAY OF CENTRAL IOWA | Des Moines | — | $25.7M | 6 |
| GOODWILL INDUSTRIES OF CENTRAL IOWA | Johnston | J32Z Employment | $25.7M | 2 |
| DES MOINES CHRISTIAN SCHOOL | Urbandale | B20 Education | $25.7M | 6 |
| SCHWARTZ FAMILY FOUNDATION | Fairfield | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $25.6M | 0 |
| QUAD CITIES COMMUNITY FOUNDATION | Bettendorf | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $25.3M | 4 |
| UKPEAGVIK INUPIAT CORP VEBA | Des Moines | Y43 Mutual & Membership Benefit | $25.3M | 1 |
| WALDORF LUTHERAN COLLEGE | Forest City | B82 Education | $25.1M | 0 |
| PEOPLES COMMUNITY HEALTH CLINIC | Waterloo | E32Z Health Care | $25M | 11 |
| NATIONAL PORK PRODUCERS COUNCIL | Des Moines | — | $24.9M | 10 |
| IOWA ASSOCIATION OF ELECTRIC COOPERATIVE | Urbandale | Y43 Mutual & Membership Benefit | $24.8M | 0 |
| IFBF PROPERTY MANAGEMENT INC | West Des Moines | — | $24.4M | 6 |
| CONSUMERS ENERGY | Marshalltown | — | $24.3M | 16 |
| ALLAMAKEE-CLAYTON ELECTRIC | Postville | — | $24.3M | 16 |
| RATHBUN REGIONAL WATER | Centerville | — | $24.2M | 8 |
| MAINSTREAM LIVING INC | Ames | P800 Human Services | $24.1M | 3 |
| BALANCE AUTISM | Altoona | P820 Human Services | $24.1M | 6 |
| IEC HOTEL CORPORATION | West Des Moines | S31 Community Improvement & Capacity Building | $24.1M | 0 |
| The Stanley Foundation dba | Muscatine | T20Z Philanthropy, Voluntarism & Grantmaking Foundations | $23.8M | 5 |
