| UPPER IOWA UNIVERSITY | Fayette | B42Z Education | $48.4M | 7 |
| Siouxland Community Health Center | Sioux City | E32Z Health Care | $48.2M | 12 |
| GOODWILL OF THE GREAT PLAINS | Sioux City | J320 Employment | $47.4M | 4 |
| FOOD BANK OF IOWA | Des Moines | K31Z Food, Agriculture & Nutrition | $47.2M | 1 |
| GRACELAND UNIVERSITY | Lamoni | B430 Education | $46.8M | 11 |
| FOUR OAKS FAMILY AND CHILDREN'S SERVICES | Cedar Rapids | P400 Human Services | $46.6M | 4 |
| SYSTEMS UNLIMITED INC | Iowa City | P73Z Human Services | $45.5M | 2 |
| RIVER BEND FOOD RESERVOIR | Davenport | K310 Food, Agriculture & Nutrition | $45.4M | 6 |
| Richard O Jacobson Foundation | Des Moines | — | $45M | 0 |
| CENTRAL IOWA JUVENILE DETENTION CENTER | Eldora | — | $44.5M | 5 |
| IOWA STATE ASSOCIATION OF COUNTIES | West Des Moines | — | $44.4M | 6 |
| Maquoketa Valley Rural Electric Cooperat | Anamosa | — | $44M | 16 |
| IMPACT COMMUNITY ACTION | Des Moines | — | $43.1M | 5 |
| L&O POWER COOPERATIVE INC | Rock Rapids | — | $43.1M | 13 |
| MERCY MEDICAL CENTER - CENTERVILLE | Centerville | E22 Health Care | $42.2M | 11 |
| SOUTH SLOPE COOPERATIVE TELEPHONE COMPANY | North Liberty | — | $41.6M | 12 |
| GREATER IOWA CREDIT UNION | Ames | — | $41.4M | 18 |
| BRIAR CLIFF UNIVERSITY | Sioux City | B420 Education | $41M | 13 |
| COMMUNITY FOUNDATION FOR WESTERN IOWA | Council Bluffs | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $40.7M | 1 |
| MULTI-STATE LOTTERY ASSOCIATION | Johnston | W190 Public & Societal Benefit | $40.6M | 7 |
| THE MEDICAL ASSOCIATES CLINIC HEALTH | Dubuque | — | $39.9M | 11 |
| ACCESS ENERGY COOPERATIVE | Mt Pleasant | — | $39.6M | 16 |
| STEWART MEMORIAL COMMUNITY HOSPITAL | Lake City | E22Z Health Care | $39.4M | 7 |
| WESTERN HOME GROUP | Cedar Falls | L80 Housing & Shelter | $39.4M | 9 |
| GREAT RIVER HEALTH SYSTEM INC | West Burlington | E12 Health Care | $39.3M | 8 |
| MERCY MEDICAL CENTER - NEWTON | Newton | E30 Health Care | $38.2M | 9 |
| SARTORI MEMORIAL HOSPITAL INC | Cedar Falls | E220 Health Care | $38.1M | 14 |
| GOODWILL INDUSTRIES OF THE HEARTLAND | Cedar Rapids | — | $37.6M | 7 |
| HEALTH AND WELFARE TRUST FOR THE | Des Moines | J40 Employment | $37M | 1 |
| CHILDSERVE HABILITATION CENTER | Johnston | E500 Health Care | $36.8M | 7 |
| TRIVIUM LIFE SERVICES | Council Bluffs | J330 Employment | $36.7M | 7 |
| Virginia Gay Hospital Inc | Vinton | E22Z Health Care | $36.4M | 9 |
| LINN AREA CREDIT UNION | Cedar Rapids | — | $36M | 10 |
| IOWA DONOR NETWORK | North Liberty | Z99Z Unknown | $36M | 10 |
| RURAL IOWA COMMUNITY HEALTH | Onawa | Y43 Mutual & Membership Benefit | $35.6M | 8 |
| ASCENTRA CREDIT UNION | Bettendorf | — | $35.4M | 6 |
| DUBUQUE RACING ASSOCIATION LTD | Dubuque | — | $35.3M | 8 |
| ALEGENT HEALTH-COMMUNITY MEMORIAL | Missouri Valley | E22Z Health Care | $34.4M | 11 |
| BUTLER COUNTY RURAL ELECTRIC COOPERATIVE | Allison | — | $34.3M | 15 |
| PRAIRIE ENERGY COOPERATIVE | Clarion | Y22 Mutual & Membership Benefit | $34.2M | 14 |
| BURGESS HEALTH CENTER | Onawa | E220 Health Care | $33.9M | 10 |
| THE SEIDLER FOUNDATION | Des Moines | — | $33.1M | 0 |
| YOUTH AND SHELTER SERVICES INC | Ames | P300 Human Services | $33M | 4 |
| BOYS & GIRLS HOME RESIDENTIAL TREATMENT CENTERS INC | Sioux City | F300 Mental Health & Crisis Intervention | $32.7M | 2 |
| TONY SCHNEIDERMAN CHARITABLE TR | George | P20 Human Services | $32.6M | 0 |
| LUTHERAN SERVICES IN IOWA INC | Des Moines | P32 Human Services | $32.5M | 5 |
| PLUMBERS & STEAMFITTERS LOCAL | Des Moines | — | $32.5M | 1 |
| THOMAS & LINDA KOEHN FOUNDATION | Des Moines | — | $32.4M | 0 |
| IOWA PRESCRIPTION DRUG CORPORATION | Grimes | E80 Health Care | $32.4M | 1 |
| ABBE CENTER FOR COMMUNITY MENTAL HEALTH | Hiawatha | F32Z Mental Health & Crisis Intervention | $32.3M | 11 |
