| EXCEPTIONAL PERSONS INC | Waterloo | P820 Human Services | $23.5M | 7 |
| IOWA CORN PROMOTION BOARD | Johnston | — | $22.9M | 10 |
| Crescent Community Health Center | Dubuque | E30 Health Care | $22.7M | 9 |
| MERCY HEALTH NETWORK INC | Des Moines | — | $22.4M | 15 |
| WESLEY COMMUNITY SERVICES INC | Johnston | P81 Human Services | $22.4M | 5 |
| EMPOWERING ABILITIES | Davenport | F33Z Mental Health & Crisis Intervention | $22.2M | 12 |
| NORTHEAST IOWA FOOD BANK | Waterloo | K31Z Food, Agriculture & Nutrition | $22.2M | 1 |
| IOWA PRESCRIPTION DRUG CORPORATION | Grimes | E80 Health Care | $22.2M | 1 |
| GOOD SHEPHERD GERIATRIC CENTER INC | Mason City | G94 Diseases, Disorders & Medical Disciplines | $22M | 7 |
| THE METH-WICK COMMUNITY INC | Cedar Rapids | P750 Human Services | $21.8M | 4 |
| LORING HOSPITAL | Sac City | E22 Health Care | $21.8M | 5 |
| MERCY FOUNDATION OF DES MOINES IOWA | Des Moines | E120 Health Care | $21.7M | 8 |
| NISHNABOTNA VALLEY RURAL ELECTRIC COOPERATIVE | Harlan | — | $21.5M | 13 |
| ORCHARD PLACE | Des Moines | F80 Mental Health & Crisis Intervention | $21.4M | 8 |
| BETHANY MANOR INC AND AFFILIATES | Story City | E91Z Health Care | $21M | 8 |
| IOWA LIFE AND HEALTH INSURANCE GUARANTY ASSOCIATION | Des Moines | — | $20.6M | 0 |
| BAYLOR COLLEGE OF MEDICINE EMPLOYER - CONTRIBUTION VEBA TR | Clinton | — | $20.3M | 2 |
| GOODWILL INDUSTRIES OF NORTHEAST IOWA INC | Waterloo | J32Z Employment | $20.2M | 5 |
| MARTIN HEALTH CENTER INC | Cedar Falls | P750 Human Services | $20.1M | 5 |
| PORTLAND GENERAL ELECTRIC COMPANY VEBA | Des Moines | Y43 Mutual & Membership Benefit | $19.9M | 1 |
| PREMIER CREDIT UNION | Des Moines | — | $19.9M | 7 |
| TANAGER PLACE | Cedar Rapids | F31Z Mental Health & Crisis Intervention | $19.8M | 7 |
| Mercy Hospital Iowa City Iowa | Iowa City | E220 Health Care | $19.7M | 8 |
| SUMNER COMMUNITY CLUB | Sumner | E22Z Health Care | $19.3M | 6 |
| ON WITH LIFE INC | Ankeny | — | $19.3M | 9 |
| INSTITUTE FOR COMMUNITY ALLIANCES | Des Moines | L80Z Housing & Shelter | $19.3M | 2 |
| COTTAGE GROVE PLACE | Cedar Rapids | P750 Human Services | $19.1M | 2 |
| VOCATIONAL DEVELOPMENT CENTER INC | Council Bluffs | S30 Community Improvement & Capacity Building | $19M | 0 |
| MERCY HOSPITAL OF FRANCISCAN SISTERS INC | Oelwein | E22Z Health Care | $19M | 13 |
| THE KRELL INSTITUTE | Ames | B500 Education | $18.9M | 5 |
| INFINITY HEALTH | Leon | E39Z Health Care | $18.9M | 11 |
| COMPREHENSIVE SYSTEMS INC | Charles City | P700 Human Services | $18.8M | 4 |
| NEW HOPE VILLAGE | Carroll | — | $18.8M | 10 |
| COMMUNITY & FAMILY RESOURCES | Fort Dodge | F20 Mental Health & Crisis Intervention | $18.7M | 3 |
| EXIRA CARE CENTER | Exira | P20 Human Services | $18.7M | 3 |
| Future Cryonics Foundation | Cedar Rapids | H99 Medical Research | $18.6M | 0 |
| COMMUNITY ACTION OF EASTERN IOWA | Davenport | S200 Community Improvement & Capacity Building | $18.3M | 3 |
| PRACTICAL FARMERS OF IOWA | Ames | — | $18.3M | 2 |
| COMMUNITY FOUNDATION OF GREATER MUSCATINE | Muscatine | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $18.2M | 1 |
| PEO Foundation | Des Moines | B122 Education | $18.2M | 0 |
| MERCY COLLEGE OF HEALTH SCIENCES | Des Moines | X220 Religion-Related | $18.2M | 10 |
| TIP RURAL ELECTRIC COOPERATIVE | Brooklyn | — | $18.1M | 16 |
| Vera French Community Mental Health Center Inc | Davenport | F320 Mental Health & Crisis Intervention | $18.1M | 8 |
| NEUBRIDG INC SUPPLEMENTAL UNEMPLOYMENT COMPENSATION BENEFIT PLAN & TRUST | Norwalk | Y24 Mutual & Membership Benefit | $18.1M | 1 |
| IOWA REGIONAL UTILITIES ASSOCIATION | Newton | — | $18M | 11 |
| CEDAR FALLS LUTHERAN HOME | Cedar Falls | L99Z Housing & Shelter | $17.8M | 6 |
| DES MOINES GOLF AND COUNTRY CLUB | West Des Moines | — | $17.8M | 6 |
| CHRISTIAN RETIREMENT HOMES INC | Davenport | P750 Human Services | $17.5M | 4 |
| ROY J CARVER CHARITABLE TRUST | Muscatine | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $17.5M | 10 |
| UPPER DES MOINES OPPORTUNITY INC | Graettinger | S200 Community Improvement & Capacity Building | $17.5M | 2 |
