| EYERLY-BALL COMMUNITY MENTAL HEALTH SERVICES | Des Moines | F32Z Mental Health & Crisis Intervention | $17.5M | 8 |
| IOWA JOBS FOR AMERICA'S GRADUATES INC | Des Moines | O50 Youth Development | $17.3M | 5 |
| COMMUNITY CRISIS SERVICES AND FOOD BANK | Iowa City | P60Z Human Services | $17.2M | 4 |
| COMMUNITY HOSPITAL INC | Hamburg | E22 Health Care | $17.1M | 7 |
| GUTHRIE COUNTY RURAL ELECTRIC COOP ASSN | Guthrie Center | — | $17.1M | 13 |
| RACCOON VALLEY ELECTRIC COOPERATIVE | Glidden | W80 Public & Societal Benefit | $17M | 14 |
| CITIZENS COMMUNITY CREDIT UNION | Fort Dodge | — | $17M | 5 |
| EMC Insurance Foundation | Des Moines | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $16.9M | 2 |
| Hillcrest Family Services Inc | Dubuque | P400 Human Services | $16.9M | 5 |
| YOUNG MEN'S CHRISTIAN ASSOCIATION OF THE IOWA MISSISSIPPI VALLEY | Davenport | P270 Human Services | $16.8M | 4 |
| AREA SUBSTANCE ABUSE COUNCIL INC | Cedar Rapids | F22Z Mental Health & Crisis Intervention | $16.8M | 2 |
| VILLAGE NORTHWEST UNLIMITED | Sheldon | P700 Human Services | $16.7M | 3 |
| THE LUTHERAN HOMES SOCIETY | Muscatine | — | $16.6M | 5 |
| COMMUNITY ACTION OF SOUTHEAST IOWA | Burlington | — | $16.4M | 2 |
| LUTHER PARK HEALTH CARE CENTER INC | Des Moines | — | $16.4M | 7 |
| HEALTHALLIANCE BENEFIT PLAN | Urbandale | Y20Z Mutual & Membership Benefit | $16.2M | 0 |
| HOPE MINISTRIES | Des Moines | P85 Human Services | $16.2M | 4 |
| MID-IOWA COMMUNITY ACTION INC | Marshalltown | S20Z Community Improvement & Capacity Building | $16.2M | 3 |
| CHILDREN AND FAMILIES OF IOWA | Des Moines | P33Z Human Services | $16.1M | 5 |
| LINK ASSOCIATES | West Des Moines | — | $16.1M | 6 |
| EMPLOYER CONTRIBUTION VEBA TRUST SOUTHERN METHODIST UNIVERSITY | Clinton | Y43 Mutual & Membership Benefit | $16.1M | 2 |
| The Lutheran Home for the Aged Association-East | Vinton | — | $16.1M | 1 |
| COMMUNITY FOUNDATION OF GREATER DUBUQUE | Dubuque | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $16M | 3 |
| KAHL HOME FOR THE AGED AND INFIRM | Davenport | E910 Health Care | $15.9M | 6 |
| MEMBERS1ST COMMUNITY CREDIT UNION | Ames | — | $15.9M | 4 |
| THE YOUNG MENS CHRISTIAN ASSOCIATION OF GREATER DES MOINES | Des Moines | — | $15.9M | 3 |
| WESTERN HOME SERVICES INC | Cedar Falls | L80 Housing & Shelter | $15.8M | 10 |
| Iowa Legal Aid | Des Moines | I80 Crime & Legal-Related | $15.8M | 5 |
| KYLE J & SHARON KRAUSE FAMILY FOUNDATION | Des Moines | Q30 International, Foreign Affairs & National Security | $15.8M | 0 |
| CHILDSERVE INC | Johnston | — | $15.8M | 10 |
| EASTERN IOWA HEALTH CENTER | Cedar Rapids | E32 Health Care | $15.8M | 10 |
| OSCEOLA COMMUNITY HOSPITAL INC | Sibley | — | $15.6M | 6 |
| FARMERS ELECTRIC COOPERATIVE INC | Greenfield | — | $15.6M | 16 |
| SOUTHERN IOWA ELECTRIC COOPERATIVE INC | Bloomfield | — | $15.5M | 11 |
| IOWA PBS FOUNDATION | Johnston | — | $15.5M | 6 |
| DISCOVERY LIVING INC | Cedar Rapids | P73Z Human Services | $15.5M | 2 |
| IOWA HOSPITAL ASSOCIATION | Des Moines | — | $15.5M | 8 |
| CEDAR RAPIDS ELECTRICAL WORKERS LOCAL #405 HEALTH AND WELFARE FUND | Cedar Rapids | — | $15.5M | 1 |
| FIRST RESOURCES CORPORATION | Ottumwa | J30Z Employment | $15.4M | 0 |
| THE FAMILY CREDIT UNION | Davenport | — | $15.3M | 2 |
| EASTER SEAL SOCIETY OF IOWA INC | Des Moines | — | $15.3M | 2 |
| GREATER CEDAR RAPIDS COMMUNITY FOUNDATION | Cedar Rapids | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $15.3M | 5 |
| CLARKE ELECTRIC COOPERATIVE INC | Osceola | — | $15.3M | 16 |
| NORTHCREST INC | Ames | P750 Human Services | $15.1M | 4 |
| HARRISON COUNTY RURAL ELECTRIC COOPERATIVE | Woodbine | W80Z Public & Societal Benefit | $15.1M | 18 |
| HOPE HAVEN AREA DEVELOPMENT CENTER CORPORATION | Burlington | P82Z Human Services | $15M | 1 |
| WESTERN IOWA POWER COOPERATIVE | Denison | — | $14.9M | 18 |
| IOWA STATE FAIR FOUNDATION | Des Moines | N520 Recreation & Sports | $14.8M | 2 |
| GOOD NEIGHBOR SOCIETY | Manchester | — | $14.8M | 1 |
| GOLF CHARITABLE FOUNDATION OF GREATER DES MOINES | Des Moines | T19 Philanthropy, Voluntarism & Grantmaking Foundations | $14.6M | 1 |
