| BROOKS SKILLED NURSING FACILITY A INC | Jacksonville | E91 Health Care | $33.9M | 8 |
| ODYSSEY CHARTER SCHOOL INC | Palm Bay | B29 Education | $33.7M | 0 |
| FACULTY ASSOCIATES INCORPORATED | Gainesville | B99 Education | $33.7M | 7 |
| MICCOSUKEE CORPORATION | Miami | P20Z Human Services | $33.7M | 7 |
| Max Planck Florida Corporation | Jupiter | H05 Medical Research | $33.5M | 11 |
| BRIGHTSIDE FOUNDATION INC | Lantana | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $33.4M | 0 |
| RENAISSANCE ARTS AND EDUCATION INC | Palmetto | B24Z Education | $33.3M | 7 |
| NEW HORIZONS OF THE TREASURE COAST INC | Ft Pierce | F300 Mental Health & Crisis Intervention | $33.1M | 11 |
| Eastern States Veterinary Association Inc | Orlando | B034 Education | $32.9M | 22 |
| Givers Legacy Foundation Inc | Clearwater | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $32.9M | 0 |
| Hope and Help Center of Central Florida Inc | Orlando | — | $32.8M | 6 |
| PEDIATRIC MEDICAL SERVICES OF FLORIDA INC | Jacksonville | E022 Health Care | $32.7M | 18 |
| DR STANLEY AND PEARL GOODMAN JFS OF BROWARD COUNTY INC | Davie | — | $32.5M | 6 |
| C Herman and Mary Virginia Terry Foundation | Jacksonville | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $32.4M | 2 |
| HOSPICE OF ST FRANCIS INC | Titusville | P74Z Human Services | $32.3M | 10 |
| Tampa Bay Convention & Visitors Bureau Inc | Tampa | — | $32.3M | 5 |
| INDIAN RIVER COMMUNITY FOUNDATION INC | Vero Beach | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $32.3M | 1 |
| RUTH ECKERD HALL INC | Clearwater | A610 Arts, Culture & Humanities | $32.2M | 5 |
| RURAL HEALTH CARE INC | Palatka | E39Z Health Care | $32.2M | 11 |
| JBJ FOUNDATION INC C/O CARISSA TEPOVICH | Coral Gables | T21 Philanthropy, Voluntarism & Grantmaking Foundations | $32.1M | 0 |
| BOYS & GIRLS CLUB OF PBC | West Palm Beach | O230 Youth Development | $32M | 6 |
| GIFT OF LIFE MARROW REGISTRY INC | Boca Raton | E99Z Health Care | $31.9M | 18 |
| YOUNG MEN'S CHRISTIAN ASSOCIATION OF THE SUNCOAST INC | Clearwater | P27Z Human Services | $31.9M | 7 |
| UNITED WAY OF BROWARD COUNTY INC | Fort Lauderdale | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $31.9M | 7 |
| FLORIDA CITRUS SPORTS EVENTS INC | Orlando | N60 Recreation & Sports | $31.9M | 10 |
| TREASURE COAST COMMUNITY HEALTH INC | Vero Beach | E39Z Health Care | $31.9M | 10 |
| STATE CHARTERED CREDIT UNIONS IN FLORIDA FLORIDA CENTRAL CREDIT UNION | Tampa | — | $31.8M | 8 |
| SalusCare Inc | Fort Myers | — | $31.7M | 10 |
| INTL ASSN AMUSEMENT PARKS & ATTRACTIONS | Orlando | — | $31.7M | 11 |
| FRATERNAL ORDER OF POLICE MIAMI LODGE #20 INSURANCE TRUST FUND | Miami | — | $31.6M | 0 |
| HUI HULIAU | Tampa | B82 Education | $31.5M | 3 |
| Jupiter Medical Center Physicians Group Inc | Jupiter | E19 Health Care | $31.4M | 10 |
| Out-of-Door Academy of Sarasota Inc | Sarasota | B200 Education | $31.3M | 7 |
| THE INTERNATIONAL SEAKEEPERS SOCIETY INC | Coral Gables | U20 Science & Technology | $31.2M | 1 |
| UNIVERSITY OF WEST FLORIDA FOUNDATION INC | Pensacola | B110 Education | $31.1M | 11 |
| RIVIERA COUNTRY CLUB OF CORAL GABLES INC | Coral Gables | — | $31.1M | 15 |
| PATIENT BUSINESS & FINANCIAL SERVICES INC | Daytona Beach | — | $31M | 7 |
| PLANNED PARENTHOOD OF SOUTHWEST AND CENTRAL FLORIDA INC | Sarasota | E420 Health Care | $30.9M | 31 |
| RURAL NEIGHBORHOODS INC | Florida City | L400 Housing & Shelter | $30.9M | 0 |
| EAST COAST ZOOLOGICAL SOCIETY OF FLORIDA INC | Melbourne | D500 Animal-Related | $30.8M | 6 |
| ST2 Foundation Inc | Sunny Isles Beach | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $30.6M | 0 |
| THE MORSELIFE HOUSING CORPORATION INC | West Palm Beach | P81 Human Services | $30.6M | 6 |
| St Andrews University | Babson Park | — | $30.5M | 7 |
| NORTHSTAR ACADEMIESINC | Fort Lauderdale | B29 Education | $30.5M | 3 |
| NEMA FOUNDATION INC | Sarasota | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $30.5M | 1 |
| EARLY LEARNING COALITION OF NORTHWEST FLORIDA INC | Marianna | B99 Education | $30.5M | 1 |
| AGENCY FOR COMMUNITY TREATMENT SERVICES INC | Tampa | F200 Mental Health & Crisis Intervention | $30.2M | 4 |
| OCEAN HABITAT AND WILDLIFE RESCUE INC | West Palm Beach | D20 Animal-Related | $30.2M | 1 |
| CARROLLWOOD DAY SCHOOL EDUCATION INC | Tampa | B24Z Education | $30.2M | 6 |
| The PATOCS Foundation | Marco Island | T20Z Philanthropy, Voluntarism & Grantmaking Foundations | $30.1M | 0 |
