| HOSPICE OF ST FRANCIS INC | Titusville | P74Z Human Services | $31.8M | 15 |
| FRATERNAL ORDER OF POLICE MIAMI LODGE #20 INSURANCE TRUST FUND | Miami | — | $31.6M | 0 |
| AVOW Hospice Inc | Naples | P74Z Human Services | $31.5M | 9 |
| BROOKS HOME CARE ADVANTAGE INC | Jacksonville | E50 Health Care | $31.4M | 8 |
| Jupiter Medical Center Physicians Group Inc | Jupiter | E19 Health Care | $31.4M | 10 |
| Out-of-Door Academy of Sarasota Inc | Sarasota | B200 Education | $31.3M | 7 |
| NAPLES CHILDREN AND EDUCATION FOUNDATION | Naples | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $31.3M | 5 |
| South Florida Hotel & Culinary Employee Welfare Fund | Pembroke Pines | — | $31.3M | 0 |
| HUI HULIAU | Tampa | B82 Education | $31.1M | 7 |
| UNIVERSITY OF WEST FLORIDA | Pensacola | B110 Education | $31.1M | 11 |
| RIVIERA COUNTRY CLUB OF CORAL GABLES INC | Coral Gables | — | $31.1M | 15 |
| PATIENT BUSINESS & FINANCIAL | Daytona Beach | — | $31M | 7 |
| Early Learning Coalition of Alachua County Inc | Gainesville | B21 Education | $31M | 3 |
| MAYFLOWER RETIREMENT CENTER INC | Winter Park | P750 Human Services | $30.9M | 8 |
| RURAL NEIGHBORHOODS INC | Florida City | L400 Housing & Shelter | $30.9M | 0 |
| EAST COAST ZOOLOGICAL | Melbourne | D500 Animal-Related | $30.8M | 6 |
| ST2 Foundation Inc | Sunny Isles Beach | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $30.6M | 0 |
| THE MORSELIFE HOUSING CORPORATION INC | West Palm Beach | P81 Human Services | $30.6M | 6 |
| St Andrews University | Babson Park | — | $30.5M | 7 |
| CARROLLWOOD DAY SCHOOL | Tampa | B24Z Education | $30.5M | 5 |
| EARLY LEARNING COALITION OF | Marianna | B99 Education | $30.5M | 1 |
| AGENCY FOR COMMUNITY TREATMENT | Tampa | F200 Mental Health & Crisis Intervention | $30.2M | 4 |
| OCEAN HABITAT AND WILDLIFE RESCUE INC | West Palm Beach | D20 Animal-Related | $30.2M | 1 |
| Tree of Knowledge Learning Academy Inc | Miami Beach | B999 Education | $30.1M | 0 |
| St Joseph's Enterprises Inc | Tampa | — | $30.1M | 10 |
| OCHO FOUNDATION | Miami | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $30M | 0 |
| Southeastern Guide Dogs Inc | Palmetto | P860 Human Services | $30M | 9 |
| LIONS WORLD VISION INSTITUTE INC | Tampa | — | $30M | 8 |
| FISH & WILDLIFE FOUNDATION OF FLORIDA I | Tallahassee | D123 Animal-Related | $30M | 4 |
| WESLEY MANOR INC | Orlando | P750 Human Services | $29.8M | 8 |
| Big Bend Hospice Inc | Tallahassee | Z99 Unknown | $29.5M | 9 |
| PROJECT HEALTH INC | Sumterville | E39Z Health Care | $29.5M | 13 |
| THE AGRICULTURAL AND LABOR PROGRAM INC | Winter Haven | K300 Food, Agriculture & Nutrition | $29.4M | 2 |
| BAY VILLAGE OF SARASOTA INC | Sarasota | P750 Human Services | $29.3M | 8 |
| HOLMES COUNTY HOSPITAL CORPORATION | Bonifay | E22 Health Care | $29.3M | 9 |
| PARC INC | St Petersburg | P820 Human Services | $29.2M | 7 |
| AMERICA'S SECOND HARVEST | Tallahassee | K31Z Food, Agriculture & Nutrition | $29M | 3 |
| St Anthony's Professional Buildings & Services Inc | Tampa | — | $29M | 8 |
| IAFF LOCAL 587 HEALTH INSURANCE | Miami | Y43 Mutual & Membership Benefit | $29M | 5 |
| FLNC Inc | Altamonte Springs | — | $28.9M | 10 |
| Jacksonville Symphony Association Inc | Jacksonville | A690 Arts, Culture & Humanities | $28.8M | 6 |
| Habitat for Humanity for Lee and | Fort Myers | L20Z Housing & Shelter | $28.8M | 3 |
| AdventHealth Foundation Inc | Altamonte Springs | E120 Health Care | $28.6M | 15 |
| PGA TOUR FIRST TEE FOUNDATION INC | Ponte Vedra Beach | N6A Recreation & Sports | $28.5M | 7 |
| KLORFINE FOUNDATION | Riviera Beach | — | $28.5M | 0 |
| AACSB INTERNATIONAL - THE ASSOCIATION TO | Tampa | B51Z Education | $28.5M | 10 |
| BLISS CARES INC | Orlando | H81 Medical Research | $28.4M | 0 |
| EARLY LEARNING COALITION OF THE | Crystal River | B192 Education | $28.4M | 2 |
| TRUE NORTH CLASSICAL ACADEMY INC | Miami | B20 Education | $28.4M | 6 |
| ESCAMBIA COUNTY SCHOOL READINESS | Pensacola | B012 Education | $28.3M | 1 |
