| ASTRAZENECA PATIENT ASSISTANCE | Wilmington | E80 Health Care | $4.6B | 0 |
| CHRISTIANA CARE HEALTH SERVICES INC | Wilmington | E22 Health Care | $2.7B | 18 |
| AMERICAN ONLINE GIVING FOUNDATION INC | Newark | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $2.1B | 0 |
| UNIVERSITY OF DELAWARE | Newark | — | $1.5B | 28 |
| FUND FOR POLICY REFORM | Wilmington | S80 Community Improvement & Capacity Building | $978.1M | 3 |
| BAYHEALTH MEDICAL CENTER INC | Dover | E220 Health Care | $978M | 21 |
| BEEBE MEDICAL CENTER INC | Lewes | E220 Health Care | $597.7M | 22 |
| Shelby Cullom Davis Charitable Fund Inc | Wilmington | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $487.1M | 1 |
| DELAWARE STATE UNIVERSITY | Dover | B430 Education | $250.4M | 24 |
| DELAWARE ELECTRIC COOPERATIVE INC | Greenwood | — | $245.3M | 25 |
| UNION HOSPITAL OF CECIL COUNTY INC | Wilmington | E220 Health Care | $177.8M | 17 |
| ST FRANCIS HOSPITAL INC | Wilmington | E220 Health Care | $158.5M | 13 |
| AMERICAN GIFT FUND | Wilmington | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $155.8M | 4 |
| TIDALHEALTH NANTICOKE INC | Seaford | E220 Health Care | $153.6M | 11 |
| Five Pointe Professional Liability Insurance Company co Wilmington Trust | Wilmington | E11 Health Care | $135.4M | 9 |
| WILMINGTON UNIVERSITY INC | New Castle | B40 Education | $123.9M | 11 |
| The Essex Avenue Foundation | Wilmington | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $92.1M | 0 |
| Conexio Care Inc | Claymont | F20 Mental Health & Crisis Intervention | $69.7M | 10 |
| HAMPSHIRE FOUNDATION INC | Wilmington | K20 Food, Agriculture & Nutrition | $67.6M | 1 |
| BEEBE PHYSICIAN NETWORK INC | Lewes | E30 Health Care | $63M | 13 |
| GOODWILL INDUSTRIES OF DELAWARE | Wilmington | J32Z Employment | $57.9M | 5 |
| The Barry S Sternlicht Foundation | Wilmington | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $56.9M | 0 |
| YOUNG MEN'S CHRISTIAN ASSOCIATION | Wilmington | P270 Human Services | $56.3M | 10 |
| The Grateful Lives Foundation | Wilmington | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $55.3M | 5 |
| INTERNATIONAL FRESH PRODUCE ASSOCIATION | Newark | K03 Food, Agriculture & Nutrition | $50.5M | 12 |
| COMMUNITY SYSTEMS INC | New Castle | P70Z Human Services | $48M | 12 |
| SUSTAINABLE ENERGY UTILITY INC | Dover | C35 Environment | $45M | 0 |
| Kadist Collection Manager Inc | Wilmington | A20 Arts, Culture & Humanities | $44.7M | 1 |
| CLIMATE ARC INC | Dover | C01 Environment | $44.4M | 10 |
| UNIDEL FOUNDATION INC | Greenville | B110 Education | $43.6M | 1 |
| FOOD BANK OF DELAWARE INC | Newark | K31Z Food, Agriculture & Nutrition | $41.5M | 4 |
| CHRISTIANA CARE HOME HEALTH AND | Wilmington | E92Z Health Care | $41.5M | 11 |
| TOWER HILL SCHOOL ASSOCIATION | Wilmington | B200 Education | $39.9M | 7 |
| Moccasin Lake Foundation | Wilmington | — | $38.7M | 0 |
| ODYSSEY CHARTER SCHOOL | Wilmington | B29 Education | $35.3M | 8 |
| LIFE AT ST FRANCIS HEALTHCARE INC | Wilmington | — | $34.7M | 18 |
| WESTSIDE FAMILY HEALTHCARE INC | Wilmington | — | $34.3M | 9 |
| St Andrew's School Of Delaware Inc | Middletown | B250 Education | $34M | 9 |
| THE SUNRISE PROJECT INC | Wilmington | C60 Environment | $33.3M | 10 |
| CHILDREN AND FAMILIES FIRST | Wilmington | P30Z Human Services | $32.5M | 7 |
| DELAWARE COMMUNITY FOUNDATION INC | Wilmington | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $32.2M | 3 |
| GOLDEY-BEACOM COLLEGE | Wilmington | B43 Education | $32.1M | 9 |
| EASTER SEALS DELAWARE & MARYLAND'S | New Castle | E50Z Health Care | $31.6M | 10 |
| FOOD FOR EDUCATION FOUNDATION INC | Dover | K30 Food, Agriculture & Nutrition | $31.3M | 0 |
| Lytton-Kambara Foundation | Wilmington | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $31.2M | 0 |
| BOYS & GIRLS CLUBS OF DELAWARE INC | Wilmington | — | $31.1M | 6 |
| ASPIRA OF DELAWARE CHARTER OPERATIONS IN | Newark | B29 Education | $29.6M | 5 |
| The Morgridge Family Foundation | Wilmington | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $29.6M | 4 |
| Zia & Rabia Bhatti Foundation | Wilmington | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $27.9M | 0 |
| DELAWARE HOSPICE INC | Newark | P74Z Human Services | $25.8M | 13 |
