| ASTRAZENECA PATIENT ASSISTANCE ORGANIZATION | Wilmington | E80 Health Care | $4.6B | 0 |
| CHRISTIANA CARE HEALTH SERVICES INC | Wilmington | E22 Health Care | $3.1B | 19 |
| AMERICAN ONLINE GIVING FOUNDATION INC | Newark | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $2.1B | 0 |
| UNIVERSITY OF DELAWARE | Newark | — | $1.7B | 26 |
| BAYHEALTH MEDICAL CENTER INC | Dover | E220 Health Care | $1.1B | 19 |
| FUND FOR POLICY REFORM | Wilmington | S80 Community Improvement & Capacity Building | $978.1M | 3 |
| BEEBE MEDICAL CENTER INC | Lewes | E220 Health Care | $634.9M | 23 |
| Shelby Cullom Davis Charitable Fund Inc | Wilmington | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $487.1M | 1 |
| DELAWARE STATE UNIVERSITY | Dover | B430 Education | $266.3M | 25 |
| DELAWARE ELECTRIC COOPERATIVE INC | Greenwood | — | $254.8M | 25 |
| William T and Frances A Little Foundation | Wilmington | T99 Philanthropy, Voluntarism & Grantmaking Foundations | $239.3M | 5 |
| SMILOW FOUNDATION INC | Wilmington | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $201M | 0 |
| UNION HOSPITAL OF CECIL COUNTY INC | Wilmington | E220 Health Care | $194.5M | 15 |
| TIDALHEALTH NANTICOKE INC | Seaford | E220 Health Care | $173.3M | 11 |
| AMERICAN GIFT FUND | Wilmington | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $166.3M | 4 |
| ST FRANCIS HOSPITAL INC | Wilmington | E220 Health Care | $161.5M | 13 |
| WILMINGTON UNIVERSITY INC | New Castle | B40 Education | $156.6M | 10 |
| JTMF Foundation | Wilmington | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $149.1M | 0 |
| The Essex Avenue Foundation | Wilmington | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $92.1M | 0 |
| The Grateful Lives Foundation | Wilmington | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $84.8M | 5 |
| Five Pointe Professional Liability Insurance Company co Wilmington Trust | Wilmington | E11 Health Care | $74.4M | 10 |
| The Brandt Jackson Foundation | Wilmington | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $71.9M | 0 |
| The Henry Francis du Pont Winterthur Museum Inc | Winterthur | A510 Arts, Culture & Humanities | $69.3M | 13 |
| Conexio Care Inc | Claymont | F20 Mental Health & Crisis Intervention | $63.9M | 10 |
| BEEBE PHYSICIAN NETWORK INC | Lewes | E30 Health Care | $62.2M | 14 |
| GOODWILL INDUSTRIES OF DELAWARE AND DELAWARE COUNTY INC | Wilmington | J32Z Employment | $57.9M | 5 |
| The Barry S Sternlicht Foundation | Wilmington | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $56.9M | 0 |
| YOUNG MEN'S CHRISTIAN ASSOCIATION OF DELAWARE | Wilmington | P270 Human Services | $56.3M | 10 |
| ZEARN | Claymont | B19 Education | $54.9M | 6 |
| FOOD BANK OF DELAWARE INC | Newark | K31Z Food, Agriculture & Nutrition | $53.4M | 8 |
| INTERNATIONAL FRESH PRODUCE ASSOCIATION | Newark | K03 Food, Agriculture & Nutrition | $50.5M | 12 |
| SUSTAINABLE ENERGY UTILITY INC | Dover | C35 Environment | $48.7M | 2 |
| COMMUNITY SYSTEMS INC | New Castle | P70Z Human Services | $48M | 12 |
| Kadist Collection Manager Inc | Wilmington | A20 Arts, Culture & Humanities | $44.7M | 1 |
| CLIMATE ARC INC | Dover | C01 Environment | $44.4M | 10 |
| UNIDEL FOUNDATION INC | Greenville | B110 Education | $43.6M | 1 |
| ODYSSEY CHARTER SCHOOL | Wilmington | B29 Education | $42.3M | 8 |
| Nova Vista Foundation | Wilmington | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $40.9M | 0 |
| CHRISTIANA CARE HOME HEALTH AND COMMUNITY SERVICES INC | Wilmington | E92Z Health Care | $39.9M | 11 |
| TOWER HILL SCHOOL ASSOCIATION | Wilmington | B200 Education | $39.2M | 8 |
| Moccasin Lake Foundation | Wilmington | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $38.7M | 0 |
| LIFE AT ST FRANCIS HEALTHCARE INC | Wilmington | — | $37.2M | 16 |
| WESTSIDE FAMILY HEALTHCARE INC | Wilmington | — | $34.3M | 9 |
| ST ANDREW'S SCHOOL OF DELAWARE INC | Middletown | B250 Education | $34.3M | 10 |
| THE SUNRISE PROJECT INC | Wilmington | C60 Environment | $33.3M | 10 |
| CHILDREN AND FAMILIES FIRST | Wilmington | P30Z Human Services | $32.5M | 7 |
| DELAWARE COMMUNITY FOUNDATION INC | Wilmington | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $32.2M | 3 |
| BOYS & GIRLS CLUBS OF DELAWARE INC | Wilmington | — | $32.1M | 8 |
| EASTER SEALS DELAWARE & MARYLAND'S EASTERN SHORE INC | New Castle | E50Z Health Care | $31.6M | 10 |
| FOOD FOR EDUCATION FOUNDATION INC | Dover | K30 Food, Agriculture & Nutrition | $31.3M | 0 |
