| GREAT JOBS KANSAS CITY | Kansas City | B82 Education | $32.9M | 5 |
| M & A ELECTRIC POWER COOP | Poplar Bluff | — | $32.7M | 17 |
| ST LOUIS PAINTERS WELFARE PLAN | Maryland Heights | — | $32.5M | 0 |
| ROBERT M - BOBBY - ALLISON CHARITABLE TR | Springfield | T23 Philanthropy, Voluntarism & Grantmaking Foundations | $32.5M | 1 |
| BERGES FAMILY FOUNDATION | St Louis | W99 Public & Societal Benefit | $32.4M | 0 |
| Mercy Hospital Aurora | Aurora | E200 Health Care | $32.2M | 12 |
| FONTBONNE UNIVERSITY | St Louis | B420 Education | $32.2M | 6 |
| AREA RESOURCES FOR COMMUNITY | St Louis | P80 Human Services | $31.9M | 7 |
| THE CRAWFORD TAYLOR FOUNDATION | St Louis | T20Z Philanthropy, Voluntarism & Grantmaking Foundations | $31.6M | 0 |
| AVILA UNIVERSITY | Kansas City | B43Z Education | $31.4M | 8 |
| St Vincent Randolph Hospital Inc | St Louis | E220 Health Care | $31M | 17 |
| INFUZE CREDIT UNION | Waynesville | — | $30.9M | 9 |
| ASCENSION HEALTH SENIOR CARE | St Louis | E210 Health Care | $30.8M | 14 |
| THE AMERICAN THEATRE GUILD | Kansas City | A65 Arts, Culture & Humanities | $30.5M | 3 |
| DELTA AREA ECONOMIC OPPORTUNITY | Sikeston | — | $30.5M | 3 |
| CONCORDIA DISABILITY AND SURVIVOR PLAN | St Louis | — | $30.5M | 0 |
| YOUNG WOMEN'S CHRISTIAN ASSOCIATION | Saint Louis | — | $30.4M | 7 |
| NORTHEAST MISSOURI HEALTH COUNCIL INC | Kirksville | — | $30.3M | 11 |
| Marion and Henry Bloch Family Foundation | Kansas City | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $30.2M | 1 |
| SOUTH CENTRAL MISSOURI COMMUNITY HEALTH CENTER | Rolla | E30 Health Care | $30.1M | 11 |
| ST LOUIS ART MUSEUM FOUNDATION | St Louis | A110 Arts, Culture & Humanities | $29.7M | 7 |
| CARROLL MANOR | St Louis | — | $29.7M | 9 |
| PLACES FOR PEOPLE INCORPORATED | St Louis | F34Z Mental Health & Crisis Intervention | $29.5M | 8 |
| Macon Electric Cooperative | Macon | — | $29.5M | 16 |
| RIVER REGION CREDIT UNION | Jefferson City | — | $29.5M | 12 |
| Consolidated Electric Cooperative | Mexico | — | $29.4M | 0 |
| JASPER COUNTY SHELTERED FACILITIES ASSOCIATION INC | Joplin | — | $28.7M | 2 |
| BOARD OF TRUSTEES IBEW-NECA HOLIDAY | St Louis | — | $28.6M | 0 |
| Family Care Health Centers | St Louis | E320 Health Care | $28.6M | 14 |
| BARRY ELECTRIC COOPERATIVE INC | Cassville | — | $28.6M | 16 |
| ALEXIAN BROTHERS COMMUNITY SERVICES | St Louis | — | $28.6M | 10 |
| PEMISCOT-DUNKLIN ELECTRIC | Kennett | — | $28.4M | 16 |
| Catholic Health Association of the United States | Saint Louis | E010 Health Care | $28M | 11 |
| Seton Medical Group Inc | St Louis | E60 Health Care | $27.8M | 20 |
| The Lutheran Home for the Aged | Cape Girardeau | — | $27.7M | 5 |
| CENTRAL BANCOMPANY INC VOLUNTARY EMPLOYEES BENEFICIARY ASSN | Jefferson Cty | — | $27.7M | 0 |
| CROSSROADS CHARTER SCHOOLS | Kansas City | B29 Education | $27.6M | 5 |
| The American Optometric Association | St Louis | — | $27.6M | 22 |
| BETTY JEAN KERR PEOPLE'S HEALTH CENTERS | St Louis | E32Z Health Care | $27.5M | 12 |
| BOARD OF TRUSTEES OF IBEW VACATION FUND | St Louis | — | $27.3M | 0 |
| HUMANE SOCIETY OF MISSOURI | St Louis | D20 Animal-Related | $27.2M | 8 |
| ALEXIAN VILLAGE OF TENNESSEE | St Louis | P750 Human Services | $27M | 5 |
| ARSENAL CREDIT UNION | Arnold | — | $26.7M | 10 |
| ST VINCENT HEALTH WELLNESS AND PREVENTIVE CARE INSTITUTE INC | St Louis | E02 Health Care | $26.7M | 11 |
| THE FOOD BANK FOR CENTRAL | Columbia | K31 Food, Agriculture & Nutrition | $26.7M | 0 |
| CHARLES N SHARPE JR FOUNDATION | Smithville | X11 Religion-Related | $26.6M | 0 |
| SUNNYHILL INC | Fenton | P73Z Human Services | $26.6M | 6 |
| GATEWAY SCIENCE ACADEMY OF ST LOUIS | St Louis | B20 Education | $26.3M | 6 |
| ASCENSION VIA CHRISTI REHABILITATION HOSPITAL INC | St Louis | E22 Health Care | $26.3M | 10 |
| JOHNNY MORRIS' WONDERS OF WILDLIFE FOUNDATION | Springfield | A50 Arts, Culture & Humanities | $26.2M | 4 |
