| The Connor Group Foundation aka The Connor Group Kids and Community Partners | Miamisburg | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $46.8M | 1 |
| DIRECTION HOME OF EASTERN OHIO | Youngstown | P810 Human Services | $46.7M | 8 |
| Butler County Community Health Consortium Inc | Hamilton | E30 Health Care | $46.1M | 8 |
| Young Mens Christian Association of Greater Cincinnati | Cincinnati | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $45.8M | 9 |
| ST AUGUSTINE MANOR | Cleveland | P750 Human Services | $45.5M | 9 |
| ALVIS INC | Columbus | P72Z Human Services | $45.2M | 6 |
| OHIO CONNECTIONS ACADEMY INC | Mason | B90 Education | $45.1M | 0 |
| 1307 DAY AIR CREDIT UNION | Kettering | — | $45M | 9 |
| THE HOWLEY FOUNDATION | Cleveland | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $44.9M | 2 |
| LICKING COUNTY FOUNDATION INC | Newark | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $44.6M | 2 |
| UNIVERSITY OF NORTHWESTERN OHIO | Lima | B42Z Education | $44.4M | 17 |
| GUERNSEY-MUSKINGUM ELECTRIC COOP | New Concord | — | $44.4M | 10 |
| HOCKINGATHENSPERRY COMMUNITY ACTION | Glouster | — | $44.3M | 3 |
| KIPP COLUMBUS | Columbus | B25 Education | $44.3M | 6 |
| STEP FORWARD | Cleveland | P200 Human Services | $44.2M | 7 |
| THE MEDICAL CENTER COMPANY | Cleveland | W120 Public & Societal Benefit | $44M | 7 |
| NATIONAL GAS & OIL COOPERATIVE | Newark | — | $43.9M | 9 |
| ECHOING HILLS VILLAGE INC | Warsaw | P750 Human Services | $43.9M | 9 |
| ADAMS COUNTY REGIONAL MEDICAL CENTER | Seaman | E20 Health Care | $43.8M | 6 |
| SHEPHERD OF THE VALLEY LUTHERAN RETIREMENT SERVICES INC | Austintown | P750 Human Services | $43.6M | 6 |
| BELLEFAIRE JEWISH CHILDREN'S BUREAU | Shaker Heights | F330 Mental Health & Crisis Intervention | $43.4M | 8 |
| POMERENE HOSPITAL FKA JOEL POMERENE FOUNDATION | Millersburg | E19 Health Care | $43.4M | 7 |
| THE COLUMBUS ACADEMY | Gahanna | B200 Education | $43.3M | 7 |
| OUR LADY OF THE WAYSIDE INC | Avon | — | $43.3M | 2 |
| OHIO MEDICAL TRANSPORTATION INC | Columbus | M23 Public Safety, Disaster Preparedness & Relief | $43.1M | 8 |
| Miami University Foundation | Oxford | B110 Education | $43.1M | 10 |
| GRACEWORKS LUTHERAN SERVICES AFFILIATES GROUP RETURN | Dayton | P200 Human Services | $43M | 8 |
| MALONE UNIVERSITY | Canton | B420 Education | $43M | 9 |
| UVMC NURSING CARE INC | Dayton | E91 Health Care | $42.9M | 3 |
| EPISCOPAL RETIREMENT HOMES INC | Cincinnati | P750 Human Services | $42.8M | 12 |
| URSULINE COLLEGE | Pepper Pike | B420 Education | $42.6M | 8 |
| COLUMBUS COLLEGE OF ART & DESIGN | Columbus | B420 Education | $42.4M | 5 |
| OHIO VALLEY GOODWILL INDUSTRIES | Cincinnati | — | $42.2M | 7 |
| Hiram College | Hiram | B420 Education | $42.2M | 8 |
| FRIENDSHIP VILLAGE OF DUBLIN OHIO INC | Dublin | P750 Human Services | $42.1M | 7 |
| ZEPF CENTER | Toledo | — | $41.7M | 7 |
| HEINZERLING COMMUNITY | Columbus | P82Z Human Services | $41.4M | 7 |
| CHARLES F KETTERING FOUNDATION | Dayton | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $41.4M | 11 |
| MIDWEST ELECTRIC INC | St Marys | — | $41.3M | 17 |
| Young Men's Christian Association of Greater Toledo | Sylvania | — | $41.1M | 3 |
| JENNINGS CENTER FOR OLDER ADULTS | Garfield Heights | E910 Health Care | $41M | 5 |
| TOLEDO COMMUNITY FOUNDATION INC | Toledo | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $40.8M | 7 |
| The Children's Home of Cincinnati Ohio | Cincinnati | P700 Human Services | $40.7M | 9 |
| COLUMBUS DOWNTOWN DEVELOPMENT CORPORATION | Columbus | S30 Community Improvement & Capacity Building | $40.7M | 9 |
| Nationwide Foundation | Columbus | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $40.4M | 0 |
| OHIO HISTORICAL SOCIETY | Columbus | A800 Arts, Culture & Humanities | $40.4M | 6 |
| Greater Cincinnati Behavioral Health Services | Cincinnati | F33 Mental Health & Crisis Intervention | $39.9M | 10 |
| PATHWAYS FINANCIAL CREDIT UNION | Columbus | — | $39.7M | 21 |
| LAKE ERIE COLLEGE | Painesville | B430 Education | $39.7M | 10 |
| Bowling Green State University Foundation Inc | Bowling Green | B110 Education | $39.6M | 8 |
