| SCIOTO COUNTY COUNSELING CENTER INC | Portsmouth | F22Z Mental Health & Crisis Intervention | $45.7M | 8 |
| ST AUGUSTINE MANOR | Cleveland | P750 Human Services | $45.5M | 9 |
| OHIO CONNECTIONS ACADEMY INC | Mason | B90 Education | $45.1M | 0 |
| 1307 DAY AIR CREDIT UNION | Kettering | — | $45M | 9 |
| MALONE UNIVERSITY | Canton | B420 Education | $45M | 9 |
| COLUMBUS COLLEGE OF ART & DESIGN | Columbus | B420 Education | $45M | 7 |
| THE HOWLEY FOUNDATION | Cleveland | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $44.9M | 2 |
| LICKING COUNTY FOUNDATION INC | Newark | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $44.6M | 2 |
| AKRON CHILDREN'S HOSPITAL FOUNDATION | Akron | E110 Health Care | $44.5M | 12 |
| HOCKINGATHENSPERRY COMMUNITY | Glouster | — | $44.3M | 3 |
| WESTERN RESERVE ACADEMY | Hudson | B250 Education | $44.2M | 10 |
| FAMILY HEALTH SERVICES OF DARKE COUNTY | Greenville | E320 Health Care | $44.1M | 8 |
| NATIONAL GAS & OIL COOPERATIVE | Newark | — | $43.9M | 9 |
| ECHOING HILLS VILLAGE INC | Warsaw | P750 Human Services | $43.9M | 9 |
| ADAMS COUNTY REGIONAL MEDICAL CENTER | Seaman | E20 Health Care | $43.8M | 6 |
| NOTRE DAME COLLEGE | South Euclid | B420 Education | $43.7M | 2 |
| SHEPHERD OF THE VALLEY | Austintown | P750 Human Services | $43.6M | 6 |
| POMERENE HOSPITAL | Millersburg | E19 Health Care | $43.4M | 7 |
| THE CLEVELAND INSTITUTE OF ART | Cleveland | B420 Education | $43.4M | 9 |
| THE COLUMBUS ACADEMY | Gahanna | B200 Education | $43.3M | 7 |
| OUR LADY OF THE WAYSIDE INC | Avon | — | $43.3M | 2 |
| CRAIG AND FRANCES LINDNER CENTER OF HOPE | Mason | F31 Mental Health & Crisis Intervention | $43.1M | 5 |
| GRACEWORKS LUTHERAN SERVICES AFFILIATES | Dayton | P200 Human Services | $43M | 8 |
| UVMC NURSING CARE INC | Dayton | E91 Health Care | $42.9M | 3 |
| BELLEFAIRE JEWISH CHILDREN'S BUREAU | Shaker Heights | F330 Mental Health & Crisis Intervention | $42.9M | 8 |
| ALVIS INC | Columbus | P72Z Human Services | $42.8M | 6 |
| BUTLER COUNTY COMMUNITY HEALTH CONSORTIUM INC | Hamilton | E30 Health Care | $42.6M | 10 |
| OHIO VALLEY GOODWILL INDUSTRIES | Cincinnati | — | $42.2M | 7 |
| FRIENDSHIP VILLAGE OF DUBLIN OHIO INC | Dublin | P750 Human Services | $42.1M | 7 |
| ZEPF CENTER | Toledo | — | $41.7M | 7 |
| UNIVERSITY OF NORTHWESTERN OHIO | Lima | B42Z Education | $41.5M | 18 |
| HEINZERLING COMMUNITY | Columbus | P82Z Human Services | $41.4M | 7 |
| CHARLES F KETTERING FOUNDATION | Dayton | — | $41.4M | 11 |
| MIDWEST ELECTRIC INC | St Marys | — | $41.3M | 17 |
| Young Men's Christian Association of Greater Toledo | Sylvania | — | $41.1M | 3 |
| GUERNSEY-MUSKINGUM ELECTRIC COOP | New Concord | — | $41M | 11 |
| STARK COMMUNITY FOUNDATION | Canton | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $40.8M | 4 |
| COLUMBUS DOWNTOWN DEVELOPMENT | Columbus | S30 Community Improvement & Capacity Building | $40.7M | 9 |
| THE MEDICAL CENTER COMPANY | Cleveland | W120 Public & Societal Benefit | $40.6M | 8 |
| NAVISTAR INC RETIREE SUPPLEMENTAL | Richmond Heights | — | $40.5M | 4 |
| Nationwide Foundation | Columbus | — | $40.4M | 0 |
| EPISCOPAL RETIREMENT HOMES INC | Cincinnati | P750 Human Services | $40M | 12 |
| Greater Cincinnati Behavioral | Cincinnati | F33 Mental Health & Crisis Intervention | $39.9M | 10 |
| Ohioans United for Reproductive Rights | Columbus | — | $39.8M | 0 |
| PATHWAYS FINANCIAL CREDIT UNION | Columbus | — | $39.7M | 21 |
| CINCINNATI-HAMILTON COUNTY COMMUNITY | Cincinnati | P200 Human Services | $39.4M | 5 |
| THE OHIO MASONIC HOME | Springfield | L23Z Housing & Shelter | $39.4M | 11 |
| COLONIAL SENIOR SERVICES | Hamilton | L22 Housing & Shelter | $39.4M | 8 |
| FAIRFIELD HEALTHCARE PROFESSIONALS | Lancaster | — | $39.3M | 7 |
| YOUNG MEN'S CHRISTIAN ASSOCIATION OF | Dayton | — | $39.2M | 8 |
