| THE BELLEVUE HOSPITAL | Bellevue | E220 Health Care | $56.9M | 11 |
| BOARD OF TRUSTEES TOLEDO ELECTRICAL WELFARE FUND | Rossford | — | $56.7M | 4 |
| OHIO DISTRICT 5 AREA AGENCY ON AGING INC | Ontario | — | $56.6M | 4 |
| 718 ATOMIC CREDIT UNION INC | Piketon | — | $56.4M | 11 |
| COMMUNITY SHELTER BOARD | Columbus | — | $56.2M | 6 |
| DAVE THOMAS FOUNDATION FOR ADOPTION | Dublin | Z99Z Unknown | $56.1M | 11 |
| Heidelberg University | Tiffin | B430 Education | $56M | 12 |
| HOLMES-WAYNE ELECTRIC COOPERATIVE INC | Millersburg | — | $55.9M | 17 |
| BROTHERHOOD OF LOCOMOTIVE ENGINEERS AND TRAINMEN GROUP RETURN | Independence | — | $55.7M | 123 |
| The Western and Southern Life Insurance Company VEBA Trust | Cincinnati | — | $55.6M | 0 |
| SOMC MEDICAL CARE FOUNDATION INC | Portsmouth | E11 Health Care | $55.6M | 15 |
| INTEGRATED SERVICES FOR BEHAVIORAL HEALTH | Nelsonville | P30 Human Services | $54.9M | 11 |
| FIDELITY HEALTH CARE | Dayton | E99 Health Care | $54.7M | 11 |
| HANCOCK-WOOD ELECTRIC COOPERATIVE | North Baltimore | — | $54.1M | 18 |
| OHIO LIVING HOLDINGS | Westerville | P80 Human Services | $53.7M | 11 |
| NAVISTAR INC RETIREE SUPPLEMENTAL BENEFIT TRUST | Richmond Heights | — | $53.6M | 6 |
| NATIONAL INVENTORS HALL OF FAME INC | North Canton | A570 Arts, Culture & Humanities | $53.4M | 9 |
| MIAMI VALLEY CHILD DEVELOPMENT CENTERS | Dayton | P330 Human Services | $53.3M | 6 |
| CINCINNATI SYMPHONY ORCHESTRA | Cincinnati | A690 Arts, Culture & Humanities | $53.3M | 12 |
| LICKING MEMORIAL HEALTH PROFESSIONALS | Newark | E32 Health Care | $53M | 8 |
| FOSTORIA HOSPITAL ASSOCIATION | Toledo | — | $52.8M | 11 |
| MCCULLOUGH-HYDE MEMORIAL HOSPITAL INCORPORATED | Cincinnati | E22Z Health Care | $52.7M | 15 |
| AREA AGENCY ON AGING PLANNING AND SERVICE AREA 2 | Dayton | P81Z Human Services | $52.7M | 9 |
| OHIO LIFE & HEALTH INSURANCE GUARANTY ASSOCIATION | Dublin | — | $52.4M | 1 |
| OHIO CONFERENCE OF TEAMSTERS AND INDUSTRY HEALTH AND WELFARE TRUST | Toledo | — | $52.2M | 0 |
| Southeast Inc | Columbus | F300 Mental Health & Crisis Intervention | $52.1M | 9 |
| The George Gund Foundation | Cleveland | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $51.5M | 4 |
| PAULDING PUTNAM ELECTRIC COOPERATIVE INC | Paulding | — | $51M | 17 |
| FIVE RIVERS HEALTH CENTERS | Dayton | E32 Health Care | $50.7M | 7 |
| BUCKEYE RURAL ELECTRIC CO-OP INC | Rio Grande | — | $50.7M | 17 |
| LORAIN-MEDINA RURAL ELECTRIC COOPERATIVE INC | Spencer | — | $50.6M | 20 |
| IRONTON AND LAWRENCE COUNTY AREA COMMUNITY ACTION ORGANIZATION | Ironton | — | $50.4M | 7 |
| GOODWILL INDUSTRIES OF GREATER CLEVELAND AND EAST CENTRAL OHIO INC | Canton | — | $50.1M | 6 |
| THE TOLEDO ZOOLOGICAL SOCIETY | Toledo | D500 Animal-Related | $50M | 9 |
| SCIOTO COUNTY COUNSELING CENTER INC | Portsmouth | F22Z Mental Health & Crisis Intervention | $49.9M | 8 |
| WESTERN RESERVE ACADEMY | Hudson | B250 Education | $49.8M | 12 |
| ALTERNATIVE EDUCATION ACADEMY | Fairlawn | O50 Youth Development | $49.6M | 4 |
| CHAMBER BENEFIT ARRANGEMENT TRUST | Akron | Y43 Mutual & Membership Benefit | $49.4M | 0 |
| FAMILY HEALTH SERVICES OF DARKE COUNTY INC | Greenville | E320 Health Care | $49.4M | 8 |
| MAPLE KNOLL COMMUNITIES INC | Cincinnati | P75 Human Services | $49M | 9 |
| AREA OFFICE ON AGING OF NORTHWESTERN OHIO INC | Toledo | P810 Human Services | $49M | 5 |
| OTTERBEIN LEBANON | Lebanon | P75 Human Services | $48.8M | 10 |
| HARBOR INC | Toledo | F320 Mental Health & Crisis Intervention | $48.6M | 6 |
| CHAMPAIGN RESIDENTIAL SERVICES INC | Urbana | P73Z Human Services | $48.5M | 15 |
| OHIO DOMINICAN UNIVERSITY | Columbus | B420 Education | $48.2M | 13 |
| SOUTHEASTERN OHIO REGIONAL MEDICAL CENTER INC | Columbus | E220 Health Care | $48M | 0 |
| KCI HOLDING USA EMPLOYEE HEALTH CARE PLAN TRUST | Springfield | Y43 Mutual & Membership Benefit | $47.8M | 1 |
| EMERALD DEVELOPMENT & ECONOMIC NETWORK | Cleveland | L80Z Housing & Shelter | $47.5M | 7 |
| UNION COUNTY PHYSICIAN CORP | Marysville | E60 Health Care | $47.4M | 7 |
| SUMMIT ACADEMY MANAGEMENT | Akron | B90 Education | $46.8M | 18 |
