| DUPONT COMMUNITY CREDIT UNION | Waynesboro | — | $95.9M | 12 |
| TEAMSTERS JOINT COUNCIL NO 83 OF | Richmond | — | $95.4M | 4 |
| UNIVERSITY OF LYNCHBURG | Lynchburg | B430 Education | $93.8M | 12 |
| ETHIOPIAN COMMUNITY DEV COUNCIL INC | Arlington | P840 Human Services | $92.4M | 2 |
| WESTMINSTER CANTERBURY CORPORATION | Richmond | P750 Human Services | $92.4M | 14 |
| Community Housing Partners Corporation | Christiansburg | — | $90.3M | 11 |
| A & N ELECTRIC COOPERATIVE | Tasley | — | $87.6M | 19 |
| REHAB JV LLC | Richmond | E50 Health Care | $87.2M | 10 |
| CENTRAL VIRGINIA HEALTH SERVICES | New Canton | E32Z Health Care | $86M | 5 |
| Richfield Living | Salem | E91Z Health Care | $85.9M | 4 |
| Augusta Medical Group | Fishersville | E60 Health Care | $84.7M | 14 |
| VIRGINIA UNITED METHODIST HOMES INC | Glen Allen | P750 Human Services | $83.9M | 14 |
| MARCH OF DIMES INC | Arlington | G200 Diseases, Disorders & Medical Disciplines | $83.6M | 12 |
| TROUT UNLIMITED INC | Arlington | D330 Animal-Related | $83.5M | 9 |
| Carilion Rockbridge Community Hospital | Roanoke | E220 Health Care | $83.1M | 16 |
| CONFEDERATION OF INDIAN INDUSTRY | Arlington | — | $82.9M | 1 |
| YOUTH FOR TOMORROW FOUNDATION | Bristow | O11 Youth Development | $82.7M | 1 |
| Fringe Benefit Plan for Service Contract | McLean | Y43 Mutual & Membership Benefit | $82.5M | 0 |
| BRIDGEWATER COLLEGE | Bridgewater | B420 Education | $82M | 11 |
| NATIONAL EXPERIENCED WORKFORCE SOLUTIONS | Arlington | J33 Employment | $81.6M | 8 |
| EMORY & HENRY COLLEGE | Emory | B420 Education | $80.3M | 6 |
| MPB INC | Virginia Beach | S47 Community Improvement & Capacity Building | $78.5M | 6 |
| INTERNATIONAL INFORMATION SYSTEM | Alexandria | — | $78.3M | 14 |
| Operation Blessing International Relief and Development Corporation | Virginia Beach | Q330 International, Foreign Affairs & National Security | $78.2M | 12 |
| INTERNATIONAL FOUNDATION FOR ELECTORAL | Arlington | Q300 International, Foreign Affairs & National Security | $77.5M | 10 |
| PEOPLE FOR THE ETHICAL TREATMENT | Norfolk | D010 Animal-Related | $77.2M | 8 |
| BLUE RIDGE AREA FOOD BANK INC | Verona | — | $76.5M | 6 |
| US CIVILIAN RESEARCH & DEVELOPMENT | Arlington | U120 Science & Technology | $76.2M | 26 |
| National Automobile Dealers Association | Tysons | — | $75.9M | 12 |
| ASSOCIATION OF STATE AND TERRITORIAL | Arlington | E010 Health Care | $75M | 13 |
| VIRGINIA WESLEYAN UNIVERSITY | Virginia Beach | — | $74.3M | 11 |
| GRAFTON SCHOOL INCORPORATED | Winchester | B280 Education | $74M | 13 |
| WOLF TRAP FOUNDATION FOR THE PERFORMING | Vienna | A600 Arts, Culture & Humanities | $72.6M | 7 |
| UNITED WAY WORLDWIDE | Alexandria | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $72.4M | 16 |
| IMAGINE SCHOOLS NON-PROFIT INC | Vienna | B200 Education | $71.9M | 9 |
| WESTMINSTER CANTERBURY ON CHESAPEAKE BAY | Virginia Beach | P750 Human Services | $71.6M | 13 |
| KISSITO HEALTHCARE INC AND AFFILIATES | Roanoke | — | $71.4M | 8 |
| PACKAGING MACHINERY MANUFACTURERS | Herndon | — | $71.4M | 8 |
| Human Resources Research Organization | Alexandria | V230 Social Science | $70.4M | 21 |
| PRISON FELLOWSHIP MINISTRIES | Lansdowne | I430 Crime & Legal-Related | $68.4M | 20 |
| UNIVERSITY OF VIRGINIA COMMUNITY | Charlottesville | — | $68M | 14 |
| ONE NATION | Haymarket | W24 Public & Societal Benefit | $66.8M | 8 |
| GOODWILL OF CENTRAL AND COASTAL | Richmond | J32 Employment | $66.5M | 7 |
| VCU Health Tappahannock Hospital | Tappahannock | E20 Health Care | $66.2M | 10 |
| THE MOUNT VERNON LADIES' ASSOCIATION | Mount Vernon | A800 Arts, Culture & Humanities | $66.1M | 11 |
| RESOLVE TO SAVE LIVES INC | Alexandria | E70 Health Care | $65.6M | 11 |
| CULP FAMILY FOUNDATION | McLean | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $65.5M | 0 |
| THE COLLEGIATE SCHOOL | Richmond | B200 Education | $64.6M | 9 |
| THE NATIONAL CENTER FOR MISSING AND EXPLOITED CHILDREN | Alexandria | I24Z Crime & Legal-Related | $64.5M | 10 |
| VERSABILITY RESOURCES INC | Hampton | — | $64.1M | 12 |
