| DAVID LAWRENCE MENTAL HEALTH CENTER | Naples | F32Z Mental Health & Crisis Intervention | $35.6M | 8 |
| FLORIDIANS FOR QUALITY VIRTUAL EDUCATION INC | Tampa | B29 Education | $35.6M | 0 |
| EARLY LEARNING COALITION OF ST LUCIE | Ft Pierce | B012 Education | $35.5M | 2 |
| SARASOTA MEMORIAL HEALTHCARE FOUNDATION | Sarasota | E22I Health Care | $35.4M | 6 |
| MID FLORIDA AREA AGENCY ON AGING | Gainesville | — | $35.4M | 3 |
| SUNCOAST PACE INC | Lakewood Ranch | P81 Human Services | $35.3M | 4 |
| INSTITUTO CENTROAMERICANO DE | Coral Gables | Q99Z International, Foreign Affairs & National Security | $35.1M | 3 |
| TREASURE COAST COMMUNITY HEALTH INC | Vero Beach | E39Z Health Care | $34.9M | 10 |
| MDS FAMILY FOUNDATION INC | Maitland | C34 Environment | $34.9M | 0 |
| COMMUNITY SCHOOL OF NAPLES INC | Naples | B200 Education | $34.8M | 7 |
| Good Shepherd Hospice Inc | Temple Terrace | E90 Health Care | $34.7M | 18 |
| HEALTH FIRST ADMINISTRATIVE PLANS INC | Rockledge | — | $34.7M | 17 |
| Palm Beach County Food Bank Inc | Lake Worth | K30 Food, Agriculture & Nutrition | $34.5M | 2 |
| NORTON MUSEUM OF ART INC | West Palm Beach | A510 Arts, Culture & Humanities | $34.5M | 7 |
| NEW WORLD SYMPHONY INC | Miami Beach | B40 Education | $34.5M | 11 |
| EARLY LEARNING COALITION OF LAKE | Leesburg | B012 Education | $34.5M | 1 |
| Webber International University | Babson Park | — | $34.4M | 5 |
| JACOB ZABARA FAMILY FOUNDATION | Miami Beach | G12 Diseases, Disorders & Medical Disciplines | $34.4M | 0 |
| SALVADORAN AMERICAN HUMANITARIAN | Miami | Q330 International, Foreign Affairs & National Security | $34.3M | 1 |
| SHORECREST PREPARATORY SCHOOL INC | St Petersburg | B20 Education | $34.2M | 7 |
| ODYSSEY CHARTER SCHOOL INC | Palm Bay | B29 Education | $34.1M | 0 |
| STELLA MARIS HOUSE INC | Lauderdale Lakes | L21Z Housing & Shelter | $34M | 0 |
| BROOKS SKILLED NURSING FACILITY A INC | Jacksonville | E91 Health Care | $33.9M | 8 |
| KIPP JACKSONVILLE INC | Jacksonville | B24 Education | $33.8M | 2 |
| FACULTY ASSOCIATES INCORPORATED | Gainesville | B99 Education | $33.7M | 7 |
| MICCOSUKEE CORPORATION | Miami | P20Z Human Services | $33.7M | 7 |
| Hope and Help Center of Central Florida Inc | Orlando | — | $33.6M | 6 |
| BRIGHTSIDE FOUNDATION INC | Lantana | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $33.4M | 0 |
| LIFE CONCEPTS INC | Orlando | P82Z Human Services | $33.4M | 8 |
| NEW HORIZONS OF THE TREASURE COAST INC | Ft Pierce | F300 Mental Health & Crisis Intervention | $33.1M | 11 |
| Eastern States Veterinary Association | Orlando | B034 Education | $32.9M | 22 |
| FLORIDA COLLEGE INC | Temple Terrace | B420 Education | $32.9M | 3 |
| Givers Legacy Foundation Inc | Clearwater | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $32.9M | 0 |
| FLORIDA GULF COAST UNIVERSITY | Fort Myers | B110 Education | $32.9M | 7 |
| PEDIATRIC MEDICAL SERVICES OF FLORIDA INC | Jacksonville | E022 Health Care | $32.7M | 18 |
| SalusCare Inc | Fort Myers | — | $32.6M | 11 |
| CLINICAL HEALTH NETWORK FOR | West Palm Beach | E02 Health Care | $32.6M | 2 |
| DR STANLEY AND PEARL GOODMAN JFS | Davie | — | $32.5M | 6 |
| EAST RIDGE RETIREMENT VILLAGE INC | Gainesville | P750 Human Services | $32.4M | 2 |
| Tampa Bay Convention & Visitors Bureau | Tampa | — | $32.3M | 5 |
| INDIAN RIVER COMMUNITY FOUNDATION INC | Vero Beach | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $32.3M | 1 |
| MIAMI CEREBRAL PALSY RESIDENTIAL | Hialeah | — | $32.3M | 9 |
| RUTH ECKERD HALL INC | Clearwater | A610 Arts, Culture & Humanities | $32.2M | 5 |
| RURAL HEALTH CARE INC | Palatka | E39Z Health Care | $32.2M | 11 |
| NORTHEAST FLORIDA AREA AGENCY ON AGING | Jacksonville | — | $32.2M | 1 |
| BOYS & GIRLS CLUB OF PBC | West Palm Beach | O230 Youth Development | $32M | 6 |
| GIFT OF LIFE MARROW REGISTRY INC | Boca Raton | E99Z Health Care | $31.9M | 18 |
| YOUNG MEN'S CHRISTIAN ASSOCIATION | Clearwater | P27Z Human Services | $31.9M | 7 |
| FLORIDA CITRUS SPORTS EVENTS INC | Orlando | N60 Recreation & Sports | $31.9M | 10 |
| THE PORT ROYAL CLUB INC | Naples | — | $31.8M | 8 |
