| JEWISH LEADERSHIP ACADAMY INC | Miami | B28 Education | $39.3M | 4 |
| AdventHealth University Inc | Orlando | — | $39.1M | 13 |
| SLAM FLORIDA INC | Miami | B29 Education | $39.1M | 3 |
| AREA AGENCY ON AGING OF PALM BEACH | West Palm Beach | P81Z Human Services | $38.9M | 3 |
| SOUTH ATLANTIC ILA EMPLOYERS | Jacksonville | — | $38.9M | 0 |
| Whole Family Health Center Inc | Vero Beach | E99 Health Care | $38.9M | 8 |
| Escambia River Electric Coop Inc | Jay | — | $38.5M | 14 |
| THE LEON AND TOBY COOPERMAN FAMILY | Boca Raton | — | $38.4M | 0 |
| ORLANDO NEIGHBORHOOD IMPROVEMENT CORPORATION INC | Orlando | L200 Housing & Shelter | $38.3M | 3 |
| PALMER TRINITY PRIVATE SCHOOL INC | Palmetto Bay | — | $38.3M | 17 |
| PANCARE OF FLORIDA INC | Panama City | E30 Health Care | $38.2M | 25 |
| PEACE RIVER CENTER FOR PERSONAL | Bartow | F320 Mental Health & Crisis Intervention | $38.1M | 8 |
| Florida Institute for Human | Pensacola | U41 Science & Technology | $38M | 14 |
| Scheck Hillel Community School | North Miami Beach | — | $38M | 10 |
| ATLANTA PLUMBERS & STEAMFITTERS | Pembroke Pines | — | $37.9M | 0 |
| THE SCHOOLS OF MCKEEL ACADEMY INC | Lakeland | B20 Education | $37.7M | 5 |
| BAKER COUNTY MEDICAL SERVICES INC | Macclenny | E22 Health Care | $37.6M | 13 |
| FLORIDA EDUCATION ASSOCIATION | Tallahassee | J40 Employment | $37.4M | 16 |
| EARLY LEARNING COALITION OF MARION | Ocala | B032 Education | $37.4M | 4 |
| NASSAU-PARADISE ISLAND PROMOTION BOARD | Plantation | S41 Community Improvement & Capacity Building | $37.2M | 5 |
| Premier Community HealthCare Group Inc | Dade City | E30Z Health Care | $37M | 12 |
| BONITA BAY CLUB | Bonita Springs | N50 Recreation & Sports | $36.9M | 7 |
| ARTIS-NAPLES INC | Naples | A610 Arts, Culture & Humanities | $36.9M | 11 |
| CENTRAL FLORIDA YOUNG MEN'S CHRISTIAN | Orlando | P27 Human Services | $36.9M | 12 |
| Florida Agricultural & Mechanical Univ | Tallahassee | B110 Education | $36.8M | 4 |
| INSIGHT CREDIT UNION | Orlando | — | $36.8M | 7 |
| Edward Waters University Inc | Jacksonville | B42Z Education | $36.8M | 9 |
| GULF CARE INC | Cape Coral | P750 Human Services | $36.6M | 15 |
| SOUTHWEST FLORIDA RETIREMENT CENTER | Venice | P750 Human Services | $36.6M | 7 |
| CENTRAL FLORIDA FAMILY HEALTH CENTER INC | Sanford | — | $36.5M | 10 |
| United Food and Commercial Workers Local 1000 and Kroger Dallas Health | Pembroke Pines | — | $36.5M | 0 |
| WESTMINSTER CHRISTIAN PRIVATE | Miami | B24Z Education | $36.4M | 6 |
| Orlando Health Foundation Inc | Orlando | E110 Health Care | $36.4M | 13 |
| AREA AGENCY OF AGING OF CENTRAL FL | Orlando | P81Z Human Services | $36.4M | 2 |
| PATRIOT SERVICES GROUP INC | Jacksonville | W30 Public & Societal Benefit | $36.2M | 0 |
| MONTVERDE ACADEMY INC | Montverde | — | $36.1M | 7 |
| EARLY LEARNING COALITION OF MANATEE | Palmetto | B21 Education | $36.1M | 3 |
| COMMUNITY FOUNDATION FOR PALM BEACH AND | West Palm Beach | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $36.1M | 8 |
| CENTRAL FLORIDA CLINICAL PRACTICE | Orlando | B11 Education | $36.1M | 15 |
| Habitat for Humanity of Pinellas | Clearwater | — | $36M | 5 |
| OPERATION PAR INC | Pinellas Park | F200 Mental Health & Crisis Intervention | $36M | 9 |
| AREAWIDE COUNCIL ON AGING OF | Sunrise | P810 Human Services | $35.9M | 3 |
| BEACON COLLEGE INC | Leesburg | — | $35.9M | 7 |
| AREA AGENCY ON AGING OF | St Petersburg | P810 Human Services | $35.9M | 3 |
| INTERNATIONAL LONGSHOREMEN'S ASSOCI | Hollywood | — | $35.8M | 13 |
| THE OUNCE OF PREVENTION FUND | Tallahassee | P400 Human Services | $35.8M | 4 |
| DISCOVERY EDUCATION SERVICES INC | Orlando | B29 Education | $35.7M | 1 |
| Orlando Lutheran Towers Inc | Orlando | L22 Housing & Shelter | $35.6M | 7 |
| COMMUNITY SUPPORTS INC | Winter Park | P75 Human Services | $35.6M | 1 |
| THE CUSHMAN SCHOOL INC | Miami | B24Z Education | $35.6M | 8 |
