| DISCOVERY EDUCATION SERVICES INC | Orlando | B29 Education | $40.7M | 1 |
| OSCEOLA CONVENTION AND VISITORS BUREAU INC | Kissimmee | S41 Community Improvement & Capacity Building | $40.7M | 5 |
| PLATO ACADEMY SCHOOLS CORPORATION | Clearwater | B99 Education | $40.6M | 0 |
| The Florida Aquarium Inc | Tampa | D50 Animal-Related | $40.6M | 9 |
| THE COUNTRY CLUB AT WOODFIELD INC | Boca Raton | N6A Recreation & Sports | $40.5M | 8 |
| CARON OF FLORIDA INC | Delray Beach | F220 Mental Health & Crisis Intervention | $40.5M | 10 |
| TrustBridge Inc | Lakewood Ranch | P11 Human Services | $40.3M | 0 |
| THE ZHAO-CHEN FAMILY FOUNDATION INC | Miami | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $40.1M | 0 |
| NAPLES CHILDREN AND EDUCATION FOUNDATION INC | Naples | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $40.1M | 5 |
| NORTH FLORIDA RETIREMENT VILLAGE INC | Gainesville | P750 Human Services | $40.1M | 7 |
| THE EVERGLADES CLUB INC | Palm Beach | — | $40M | 9 |
| THE LATIN ACADEMY OF RECORDING ARTS & SCIENCES INC | Miami | — | $39.9M | 11 |
| Closehaul Capital Charitable Inc | Miami | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $39.9M | 1 |
| NORTHEAST FLORIDA AREA AGENCY ON AGING INC | Jacksonville | — | $39.9M | 1 |
| VILLA MARIA NURSING & REHABILITATION CENTER INC | North Miami | E50 Health Care | $39.8M | 10 |
| BEACON COLLEGE INC | Leesburg | — | $39.8M | 9 |
| ESCAMBIA SCHOOL DISTRICT BENEFIT TRUST | Pensacola | — | $39.8M | 3 |
| SOUTH ATLANTIC ILA EMPLOYERS DISTRICT ESCROW FUND | Jacksonville | — | $39.7M | 0 |
| Scheck Hillel Community School | North Miami Beach | — | $39.7M | 11 |
| THE INSTITUTE FOR INTERGOVERNMENTAL RESEARCH INC | Tallahassee | L20 Housing & Shelter | $39.7M | 17 |
| BAY HAVEN CHARTER ACADEMY INC | Panama City | B302 Education | $39.6M | 2 |
| EARLY LEARNING COALITION OF THE BIG BEND REGION INC | Tallahassee | B21 Education | $39.6M | 1 |
| OPERATION PAR INC | Pinellas Park | F200 Mental Health & Crisis Intervention | $39.5M | 9 |
| JEWISH COMMUNITY SERVICES OF SOUTH FLORIDA INC | Miami | P40 Human Services | $39.5M | 6 |
| CENTRAL FLORIDA FAMILY HEALTH CENTER INC | Sanford | — | $39.5M | 15 |
| THE SEMINOLE COUNTY COALITION FOR SCHOOL READINESS INC | Longwood | B112 Education | $39.4M | 1 |
| BANYAN COMMUNITY HEALTH CENTER INC | Doral | E32 Health Care | $39.4M | 15 |
| CAMILLUS HOUSE INC | Miami | — | $39.3M | 6 |
| Palm Beach County Food Bank Inc | Lake Worth | K30 Food, Agriculture & Nutrition | $39.3M | 2 |
| JACKSONVILLE ZOOLOGICAL SOCIETY INC | Jacksonville | D500 Animal-Related | $39.3M | 7 |
| Jessie Trice Community Health System Inc | Miami | E320 Health Care | $39.2M | 15 |
| Mayflower Retirement Center Inc | Winter Park | P750 Human Services | $39.1M | 8 |
| SLAM FLORIDA INC | Miami | B29 Education | $39.1M | 3 |
| BONITA BAY CLUB | Bonita Springs | N50 Recreation & Sports | $39M | 7 |
| AREA AGENCY ON AGING OF PALM BEACH TREASURE COAST INC | West Palm Beach | P81Z Human Services | $38.9M | 3 |
| SOUTH ATLANTIC ILA EMPLOYERS VACATION AND HOLIDAY FUND | Jacksonville | — | $38.9M | 0 |
| GULF CARE INC | Cape Coral | P750 Human Services | $38.7M | 13 |
| AREAWIDE COUNCIL ON AGING OF BROWARD COUNTY INC | Sunrise | P810 Human Services | $38.6M | 3 |
| Escambia River Electric Coop Inc | Jay | — | $38.5M | 14 |
| THE OUNCE OF PREVENTION FUND OF FLORIDA INC | Tallahassee | P400 Human Services | $38.3M | 5 |
| ORLANDO NEIGHBORHOOD IMPROVEMENT CORPORATION INC | Orlando | L200 Housing & Shelter | $38.3M | 3 |
| PALMER TRINITY PRIVATE SCHOOL INC | Palmetto Bay | — | $38.3M | 17 |
| PANCARE OF FLORIDA INC | Panama City | E30 Health Care | $38.2M | 25 |
| PEACE RIVER CENTER FOR PERSONAL DEVELOPMENT INC | Bartow | F320 Mental Health & Crisis Intervention | $38.1M | 8 |
| Florida Institute for Human and Machine Cognition Inc | Pensacola | U41 Science & Technology | $38M | 14 |
| BAKER COUNTY MEDICAL SERVICES INC | Macclenny | E22 Health Care | $37.6M | 13 |
| FLORIDA EDUCATION ASSOCIATION | Tallahassee | J40 Employment | $37.4M | 16 |
| SHORECREST PREPARATORY SCHOOL INC | St Petersburg | B20 Education | $37.3M | 7 |
| BROOKS HOME CARE ADVANTAGE INC | Jacksonville | E50 Health Care | $37.3M | 8 |
| CLOVERFIELDS FOUNDATION | Naples | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $37.3M | 0 |
