| YOUNG MEN'S CHRISTIAN ASSOCIATION OF SOUTH FLORIDA INC | Fort Lauderdale | — | $69.3M | 9 |
| TED ARISON FAMILY FOUNDATION USA INC | Aventura | — | $68.8M | 1 |
| LENNAR FOUNDATION | Miami | — | $68.7M | 1 |
| JACKSONVILLE POLICE AND FIREFIGHTER | Jacksonville | Y43 Mutual & Membership Benefit | $68.5M | 0 |
| University of South Florida Foundation Inc | Tampa | B110 Education | $68.5M | 16 |
| BORINQUEN HEALTH CARE CENTER INC | Miami | — | $67.6M | 9 |
| Dr Phillips Center for the | Orlando | A11 Arts, Culture & Humanities | $67.6M | 8 |
| NATIONAL ASBESTOS WORKERS MEDICAL FUND | Pembroke Pines | — | $67.5M | 5 |
| BREVARD HEALTH ALLIANCE INC | West Melbourne | E30 Health Care | $67.3M | 10 |
| CENTERSTONE OF FLORIDA INC | Bradenton | E22 Health Care | $67.1M | 10 |
| Heartland for Children Inc | Bartow | O50 Youth Development | $67.1M | 4 |
| FLORIDA WEST COAST SYMPHONY INC | Sarasota | A690 Arts, Culture & Humanities | $66.5M | 3 |
| AdventHealth Senior Care Inc | Altamonte Springs | — | $66.4M | 21 |
| MEDICAL CENTER OF DELTONA INC | Daytona Beach | E20 Health Care | $65.8M | 1 |
| South Florida Preventive Care Initiative Inc | Miami | B99 Education | $65.2M | 1 |
| STATE CHARTERED CREDIT UNIONS IN FLORIDA | Jacksonville | — | $65M | 14 |
| COMMUNITY CREDIT UNION OF FLORIDA | Rockledge | W61Z Public & Societal Benefit | $64.8M | 11 |
| Collier Health Services Inc | Immokalee | E30Z Health Care | $64.7M | 9 |
| FLORIDA RADIOLOGY IMAGING AT | Maitland | — | $64.2M | 9 |
| MERIDIAN BEHAVIORAL HEALTHCARE INC | Gainesville | F300 Mental Health & Crisis Intervention | $63.8M | 14 |
| GULF WINDS CREDIT UNION | Pensacola | W61 Public & Societal Benefit | $63M | 10 |
| NAVAL CONTINUING CARE RETIREMENT | Atlantic Beach | P750 Human Services | $62.7M | 13 |
| CYPRESS COVE AT HEALTHPARK FLORIDA INC | Fort Myers | P75 Human Services | $62.1M | 7 |
| SAINT ANDREW'S SCHOOL OF BOCA RATON INC | Boca Raton | B250 Education | $61.8M | 7 |
| Glades Electric Cooperative Inc | Moore Haven | — | $61.8M | 22 |
| Jupiter Medical Center Foundation Inc | Jupiter | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $61.6M | 10 |
| ALLIANCE FOR AGING INC | Miami | P81Z Human Services | $61.5M | 3 |
| Big Brothers Big Sisters Of America | Tampa | — | $61.4M | 9 |
| GOODWILL INDUSTRIES OF SW FLORIDA INC | Fort Myers | J32Z Employment | $61M | 8 |
| FIRST COMMERCE CREDIT UNION | Tallahassee | — | $61M | 12 |
| STATE CHARTERED CREDIT UNIONS IN FLORIDA | Tallahassee | — | $60.9M | 16 |
| SAFE CHILDREN COALITION INC | Sarasota | P270 Human Services | $60.9M | 5 |
| COLLIER COMMUNITY FOUNDATION INC | Naples | — | $59.9M | 3 |
| EARLY LEARNING COALITION OF THE BIG | Tallahassee | B21 Education | $59.3M | 1 |
| TRI-COUNTY ELECTRIC COOPERATIVE INC | Madison | — | $59.2M | 19 |
| LAKE WALES CHARTER SCHOOLS INC | Lake Wales | B29 Education | $59.2M | 9 |
| Pulte Family Charitable Foundation | Boca Raton | — | $59.2M | 4 |
| UNITED WAY MIAMI INC | Miami | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $58.7M | 11 |
| AMIKIDS INC | Tampa | I400 Crime & Legal-Related | $58.5M | 0 |
| DADE COUNTY FIRE FIGHTERS | Miami | — | $58.5M | 0 |
| TREASURE COAST FOOD BANK INC | Fort Pierce | K31Z Food, Agriculture & Nutrition | $58.4M | 1 |
| PACE CENTER FOR GIRLSINC | Jacksonville | O220 Youth Development | $57.9M | 9 |
| JOHN KNOX VILLAGE OF | Orange City | P750 Human Services | $57.5M | 7 |
| BRIGHTSTAR CREDIT UNION | Cooper City | — | $57.3M | 30 |
| MATER ACADEMY CENTRAL INC | Kissimmee | B29 Education | $57.1M | 3 |
| TROPICAL FINANCIAL CREDIT UNION | Miramar | — | $57M | 19 |
| BRIDGEPREP ACADEMY INC | Miami | B24 Education | $56.8M | 3 |
| FOUNDCARE INC | West Palm Beach | G028 Diseases, Disorders & Medical Disciplines | $56.8M | 23 |
| GENESIS HEALTH INC | Jacksonville | E210 Health Care | $56.4M | 25 |
| SUNCOAST COMMUNITY HEALTH CENTERS INC | Ruskin | — | $56.2M | 12 |
