| STATE CHARTERED CREDIT UNIONS IN FLORIDA FIRST FLORIDA CREDIT UNION | Jacksonville | — | $73.9M | 13 |
| SAINT ANDREW'S SCHOOL OF BOCA RATON INC | Boca Raton | B250 Education | $73.3M | 7 |
| THE LAURA & ISAAC PERLMUTTER FOUNDATION INC | Lake Worth | T90Z Philanthropy, Voluntarism & Grantmaking Foundations | $73.1M | 1 |
| UNITED WAY MIAMI INC | Miami | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $71.7M | 11 |
| CATHOLIC HOSPICE INC | Miami Lakes | P74Z Human Services | $71.4M | 12 |
| LAKE HIGHLAND PREPARATORY SCHOOL INC | Orlando | B25Z Education | $71.4M | 11 |
| MARGUERITE DELANY HARK FOUNDATION | Venice | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $70.9M | 3 |
| Hospice of Palm Beach County Inc | Lakewood Ranch | P74 Human Services | $70.3M | 0 |
| GIVE KIDS THE WORLD INC | Kissimmee | — | $69.6M | 9 |
| AMIKIDS INC (GROUP) | Tampa | I400 Crime & Legal-Related | $69.5M | 0 |
| Heartland for Children Inc | Bartow | O50 Youth Development | $69.5M | 5 |
| YOUNG MEN'S CHRISTIAN ASSOCIATION OF SOUTH FLORIDA INC | Fort Lauderdale | — | $69.3M | 9 |
| POLK COUNTY SCHOOL READINESS COALITION INC | Lakeland | B012 Education | $69.1M | 4 |
| BORINQUEN HEALTH CARE CENTER INC | Miami | — | $69.1M | 9 |
| TED ARISON FAMILY FOUNDATION USA INC | Aventura | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $68.8M | 1 |
| JACKSONVILLE POLICE AND FIREFIGHTER HEALTH INSURANCE TRUST | Jacksonville | Y43 Mutual & Membership Benefit | $68.5M | 0 |
| AdventHealth Home Health and Hospice Inc | Maitland | P74Z Human Services | $67.8M | 7 |
| The Marie-Josee and Henry R Kravis Foundation | Miami | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $67.6M | 0 |
| NATIONAL ASBESTOS WORKERS MEDICAL FUND FUND BOARD OF TRUSTEES | Pembroke Pines | — | $67.5M | 5 |
| COMMUNITY BASED CARE OF BREVARD INC | Rockledge | E60 Health Care | $67.4M | 5 |
| BREVARD HEALTH ALLIANCE INC | West Melbourne | E30 Health Care | $67.3M | 10 |
| ADVANTAGE ACADEMY OF HILLSBOROUGH INC | Coral Springs | B29 Education | $67M | 6 |
| MERIDIAN BEHAVIORAL HEALTHCARE INC | Gainesville | F300 Mental Health & Crisis Intervention | $66.6M | 13 |
| CENTERSTONE OF FLORIDA INC | Bradenton | E22 Health Care | $66.4M | 12 |
| ALLIANCE FOR AGING INC | Miami | P81Z Human Services | $66.4M | 3 |
| AdventHealth Senior Care Inc | Altamonte Springs | — | $66.4M | 21 |
| NAVAL CONTINUING CARE RETIREMENT FOUNDATION INC | Atlantic Beach | P750 Human Services | $66M | 12 |
| MEDICAL CENTER OF DELTONA INC | Daytona Beach | E20 Health Care | $65.8M | 1 |
| AMIKIDS INC (PARENT) | Tampa | — | $65.7M | 9 |
| South Florida Preventive Care Initiative Inc | Miami | B99 Education | $65.2M | 1 |
| COMMUNITY CREDIT UNION OF FLORIDA | Rockledge | W61Z Public & Societal Benefit | $64.8M | 11 |
| Collier Health Services Inc | Immokalee | E30Z Health Care | $64.7M | 9 |
| THE BENJAMIN PRIVATE SCHOOL INC | North Palm Beach | B20Z Education | $64M | 7 |
| GIVEWELL COMMUNITY FOUNDATION INC | Lakeland | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $63.7M | 3 |
| Lifestream Behavioral Center Inc | Leesburg | E24 Health Care | $62.5M | 7 |
| THE KOVNER FOUNDATION | Jupiter | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $62.4M | 2 |
| CYPRESS COVE AT HEALTHPARK FLORIDA INC | Fort Myers | P75 Human Services | $62.1M | 7 |
| Glades Electric Cooperative Inc | Moore Haven | — | $61.8M | 22 |
| The Jim Moran Foundation Inc | Deerfield Beach | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $61.6M | 2 |
| Jupiter Medical Center Foundation Inc | Jupiter | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $61.6M | 10 |
| COMMUNITY PARTNERSHIP FOR CHILDREN INC | Daytona Beach | P20 Human Services | $61.6M | 5 |
| DR PHILLIPS CENTER FOR THE PERFORMING ARTS INC | Orlando | A11 Arts, Culture & Humanities | $61.5M | 8 |
| Big Brothers Big Sisters Of America | Tampa | — | $61.4M | 9 |
| SUNCOAST COMMUNITY HEALTH CENTERS INC | Ruskin | — | $61.3M | 12 |
| GOODWILL INDUSTRIES OF SW FLORIDA INC | Fort Myers | J32Z Employment | $61M | 8 |
| FIRST COMMERCE CREDIT UNION | Tallahassee | — | $61M | 12 |
| STATE CHARTERED CREDIT UNIONS IN FLORIDA ENVISION CREDIT UNION | Tallahassee | — | $60.9M | 16 |
| SAFE CHILDREN COALITION INC | Sarasota | P270 Human Services | $60.9M | 5 |
| COLLIER COMMUNITY FOUNDATION INC | Naples | — | $59.9M | 3 |
| TROPICAL FINANCIAL CREDIT UNION | Miramar | — | $59.9M | 18 |
