| TRI-COUNTY ELECTRIC COOPERATIVE INC | Madison | — | $59.2M | 19 |
| LAKE WALES CHARTER SCHOOLS INC | Lake Wales | B29 Education | $59.2M | 9 |
| Pulte Family Charitable Foundation | Boca Raton | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $59.2M | 4 |
| PACE CENTER FOR GIRLSINC | Jacksonville | O220 Youth Development | $58.7M | 8 |
| TREASURE COAST FOOD BANK INC | Fort Pierce | K31Z Food, Agriculture & Nutrition | $58.4M | 1 |
| HENDERSON BEHAVIORAL HEALTH INC | Ft Lauderdale | F320 Mental Health & Crisis Intervention | $57.9M | 13 |
| DADE COUNTY FIRE FIGHTERS INSURANCE TRUST FUND | Miami | — | $57.7M | 0 |
| JOHN KNOX VILLAGE OF CENTRAL FLORIDA INC | Orange City | P750 Human Services | $57.5M | 7 |
| BRIGHTSTAR CREDIT UNION | Cooper City | — | $57.3M | 30 |
| MATER ACADEMY CENTRAL INC | Kissimmee | B29 Education | $57.1M | 3 |
| LITTLE HAVANA ACTIVITIES AND NUTRITION CENTERS OF DADE COUNTY INC | Miami | K00Z Food, Agriculture & Nutrition | $56.9M | 3 |
| FOUNDCARE INC | West Palm Beach | G028 Diseases, Disorders & Medical Disciplines | $56.8M | 23 |
| JOHN'S ISLAND CLUB INC | Indian River Shores | — | $56.6M | 10 |
| GULF COAST JEWISH FAMILY AND COMMUNITY SERVICES INC | Clearwater | — | $56.5M | 9 |
| GENESIS HEALTH INC | Jacksonville | E210 Health Care | $56.4M | 25 |
| ARBITRATION FORUMS INC | Tampa | I51Z Crime & Legal-Related | $56.4M | 15 |
| HEALTH CHOICE NETWORK OF FLORIDA INC | Miami | E32 Health Care | $56M | 14 |
| GOODWILL INDUSTRIES OF NORTH FLORIDAINC | Jacksonville | — | $55.8M | 9 |
| CIRCLES OF CARE INC | Melbourne | F300 Mental Health & Crisis Intervention | $55.7M | 13 |
| RADIANT CREDIT UNION | Gainesville | W61 Public & Societal Benefit | $55.6M | 17 |
| RIVER CITY EDUCATION SERVICES INC | Jacksonville | B29 Education | $55.5M | 1 |
| GULFSTREAM GOODWILL INDUSTRIES INC | West Palm Beach | J320 Employment | $55.5M | 9 |
| ALL FAITHS FOOD BANK INC | Sarasota | K310 Food, Agriculture & Nutrition | $55.4M | 8 |
| ACADEMIR CHARTER SCHOOL INC | Miami | B29 Education | $55M | 0 |
| PARTNERSHIP FOR STRONG FAMILIES INC | Gainesville | E60 Health Care | $54.9M | 4 |
| THE EARLY LEARNING COALITION OF FLAGLER AND VOLUSIA COUNTIES INC | Daytona Beach | B192 Education | $54.8M | 4 |
| THE GLENRIDGE ON PALMER RANCH INC | Sarasota | — | $54.6M | 5 |
| DAVID LAWRENCE MENTAL HEALTH CENTER INC | Naples | F32Z Mental Health & Crisis Intervention | $54.6M | 7 |
| GULF COAST COMMUNITY FOUNDATION INC | Venice | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $54.4M | 8 |
| FLORIDA ASSOCIATION OF REHABILITATION FACILITIES INC | Tallahassee | — | $54.4M | 3 |
| TAMPA METROPOLITAN AREA YOUNG MEN'S CHRISTIAN ASSOCIATION INC | Tampa | P270 Human Services | $54.3M | 9 |
| NORTH CENTRAL FLORIDA HOSPICE INC | Gainesville | P740 Human Services | $54.2M | 2 |
| SPECIAL OPERATIONS WARRIOR FOUNDATION | Tampa | B82 Education | $53.9M | 6 |
| Hope for Haiti Inc | Naples | Q123 International, Foreign Affairs & National Security | $53.6M | 1 |
| TAMPA BAY PERFORMING ARTS CENTER INC | Tampa | — | $53M | 10 |
| GATOR BOOSTERS INC | Gainesville | — | $52.9M | 6 |
| EARLY LEARNING COALITION OF NORTH FLORIDA INC | St Augustine | B21 Education | $52.7M | 1 |
| DEITCH FAMILY CHARITABLE SUPPORT TRUST | Miami Beach | T11 Philanthropy, Voluntarism & Grantmaking Foundations | $52.6M | 0 |
| Orlando Neighborhood Improvement Foundation Inc | Orlando | L20 Housing & Shelter | $52.6M | 3 |
| TAMPA GENERAL HOSPITAL FOUNDATION INC | Tampa | E11 Health Care | $52.5M | 2 |
| ST JOSEPH'S HOSPITAL OF TAMPA FOUNDATION INC | Tampa | E99 Health Care | $52.4M | 5 |
| MENTAL HEALTH CARE INC | Tampa | F31Z Mental Health & Crisis Intervention | $52.2M | 8 |
| SOCIETY OF ST VINCENT DE PAUL SOUTH PINELLAS INC | St Petersburg | P20 Human Services | $52.1M | 8 |
| THE BATCHELOR FOUNDATION INC | Miami Beach | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $52M | 4 |
| GREATER MIAMI CONVENTION & VISITORS | Miami | — | $51.8M | 9 |
| JAEB CENTER FOR HEALTH RESEARCH FOUNDATION INC | Tampa | Z99 Unknown | $51.6M | 9 |
| BONITA SPRINGS UTILITIES INC | Bonita Springs | — | $51.3M | 14 |
| FLORIDA PROTON THERAPY INSTITUTE INC | Jacksonville | E24 Health Care | $51.3M | 11 |
| EMPATH-STRATUM INC | Lakewood Ranch | P74 Human Services | $51.1M | 10 |
| ADRIENNE ARSHT CENTER TRUST INC | Miami | A111 Arts, Culture & Humanities | $51.1M | 7 |
