| ELISABETH C DELUCA FOUNDATION INC | Pompano Beach | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $51.1M | 0 |
| HEALTH CHOICE NETWORK INC | Miami | E60 Health Care | $51M | 12 |
| PUBLIX SUPER MARKETS CHARITIES INC | Lakeland | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $51M | 5 |
| ADVANTAGE ACADEMY OF MIAMI INC | Coral Springs | B21 Education | $51M | 5 |
| LIONS WORLD VISION INSTITUTE INC | Tampa | — | $50.6M | 9 |
| ST MATTHEWS HOUSE INC | Naples | L41 Housing & Shelter | $50.5M | 9 |
| THE BROKEN SOUND CLUB INC | Boca Raton | N50 Recreation & Sports | $50.5M | 7 |
| FEDERATION CCRC OPERATIONS CORP | Boca Raton | L22 Housing & Shelter | $50.5M | 3 |
| FLORIDA INTERNATIONAL UNIVERSITY FOUNDATION INC | Miami | B110 Education | $50.1M | 14 |
| EARLY LEARNING COALITION OF BREVARD COUNTY INC | Rockledge | B012 Education | $50.1M | 2 |
| PLYMOUTH HARBOR INC | Sarasota | E91Z Health Care | $50.1M | 12 |
| Lincoln-Marti Community Agency Inc | Miami | B95Z Education | $50M | 3 |
| THE KING'S ACADEMY INC | West Palm Beach | B24Z Education | $50M | 9 |
| FLORIDA ATLANTIC UNIVERSITY FOUNDATION INC | Boca Raton | B110 Education | $49.8M | 4 |
| WE FLORIDA FINANCIAL | Pembroke Pines | — | $49.8M | 10 |
| Hernando Pasco Hospice Inc | Temple Terrace | P74Z Human Services | $49.8M | 16 |
| YOUTH CO-OP INC | Miami | J200 Employment | $49.7M | 5 |
| Hospice of Marion County Inc | Lakewood Ranch | P74 Human Services | $49.5M | 9 |
| THE YOUNG MEN'S CHRISTIAN ASSOCIATION OF FLORIDA'S FIRST COAST INC | Jacksonville | — | $49.4M | 7 |
| JEWISH FEDERATION OF PALM BEACH COUNTY INC | West Palm Beach | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $49.4M | 9 |
| AdventHealth University Inc | Orlando | — | $49.3M | 10 |
| GULFSIDE HOSPICE INC | Land Olakes | — | $49.3M | 4 |
| FLORIDA NETWORK OF YOUTH AND FAMILY SERVICES INC | Tallahassee | P300 Human Services | $49.2M | 4 |
| APALACHEE CENTER INC | Tallahassee | F300 Mental Health & Crisis Intervention | $49.1M | 20 |
| BayCare Behavioral Health Inc | New Port Richey | F200 Mental Health & Crisis Intervention | $48.7M | 11 |
| Lowry Park Zoological Society of Tampa Inc | Tampa | D500 Animal-Related | $48.6M | 10 |
| Best Buddies International Inc | Miami | Q20 International, Foreign Affairs & National Security | $48.3M | 7 |
| WATERMAN COMMUNITIES INC | Mount Dora | P750 Human Services | $48.2M | 8 |
| THE LOWELL S & BETTY L DUNN FAMILY FOUNDATION INC | Hialeah Gardens | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $48.2M | 0 |
| FLORIDA COMMUNITY HEALTH CENTERS INC | West Palm Beach | — | $48.1M | 6 |
| AMERICAN MARITIME OFFICERS MEDICAL PLAN | Dania Beach | — | $47.8M | 17 |
| SCHOOL BOARD OF LEON COUNTY FL-VEBA VOLUNTARY EMPLOYEE BENEFICIARY ASSO | Tallahassee | — | $47.7M | 8 |
| UNITED STATES POLO ASSOCIATION INC | Wellington | — | $47.6M | 12 |
| AdventHealth Foundation Inc | Altamonte Springs | E120 Health Care | $47.4M | 20 |
| CATHOLIC CHARITIES OF THE ARCHDIOCESE OF MIAMI INC | Wilton Manors | P200 Human Services | $47.3M | 8 |
| MENTAL HEALTH RESOURCE CENTER INC | Jacksonville | E99Z Health Care | $47.2M | 7 |
| LIFE CARE PONTE VEDRA INC | Ponte Vedra Beach | P750 Human Services | $47.2M | 6 |
| SPORTS LEADERSHIP AND MANAGEMENT INC | Miami | B200 Education | $47.1M | 5 |
| FLORIDA ASSOCIATION OF REALTORS INC | Orlando | S41 Community Improvement & Capacity Building | $47.1M | 9 |
| MOUNT SINAI MEDICAL CENTER INSURANCE TRUST | Miami Beach | Y20Z Mutual & Membership Benefit | $47M | 3 |
| VOLO FOUNDATION | Palm Beach Gardens | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $46.8M | 1 |
| AMERICAN WELDING SOCIETY INC | Miami | S410 Community Improvement & Capacity Building | $46.8M | 10 |
| PALM BEACH COUNTY FIRE FIGHTERS EMPLOYEES' BENEFITS FUND | West Palm Beach | — | $46.7M | 8 |
| Oak Hammock at the University of Florida Inc | Gainesville | P750 Human Services | $46.6M | 8 |
| GENESIS HEALTH DEVELOPMENT INC | Jacksonville | — | $46.6M | 9 |
| WHOLE FAMILY HEALTH CENTER INC | Vero Beach | E99 Health Care | $46.4M | 6 |
| ESCAMBIA COMMUNITY CLINICS INC | Pensacola | E32Z Health Care | $46.4M | 12 |
| HEALTHY START MOMCARE NETWORK INC | Tallahassee | P40 Human Services | $46.4M | 3 |
| STRATUM HEALTH SYSTEM INC | Lakewood Ranch | E19 Health Care | $46.3M | 12 |
| AMERICA'S SECOND HARVEST OF THE BIG BEND INC | Tallahassee | K31Z Food, Agriculture & Nutrition | $46.2M | 4 |
