| KNAPP-SWEZEY FOUNDATION INC | Patchogue | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $51.9M | 12 |
| ACDS INC | Plainview | — | $51.9M | 7 |
| COMMUNITY ACCESS INC | New York | F33Z Mental Health & Crisis Intervention | $51.8M | 8 |
| TARGET ALS FOUNDATION INC | New York | B82 Education | $51.7M | 2 |
| UFCW LOCAL 2013 HEALTH AND WELFARE FUND | Brooklyn | Y43 Mutual & Membership Benefit | $51.7M | 0 |
| IBEW LOCAL 25 HEALTH & BENEFIT FUND | Hauppauge | — | $51.7M | 0 |
| UNIVERSITY AT BUFFALO NEUROSURGERY INC | Williamsville | E20 Health Care | $51.7M | 31 |
| GOD'S LOVE WE DELIVER INC | New York | K36Z Food, Agriculture & Nutrition | $51.6M | 13 |
| THE WOODLAWN CEMETERY | Bronx | — | $51.6M | 10 |
| ALEXIS GREGORY FOUNDATION | Harrison | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $51.6M | 0 |
| Shen Yun Performing ArtsInc | Cuddebackville | A60 Arts, Culture & Humanities | $51.5M | 2 |
| THE STELLA ORTON HOME CARE AGENCY INC | Staten Island | P440 Human Services | $51.5M | 3 |
| LITTLE FLOWER CHILDREN FAMILY SERVICES OF NEW YORK | Wading River | P20 Human Services | $51.4M | 8 |
| UNITED UNIVERSITY PROFESSIONS BENEFIT TRUST FUND | Albany | — | $51.4M | 10 |
| NYSUT MEMBER BENEFITS CATASTROPHE MAJOR MEDICAL INSURANCE TRUST | Latham | E80 Health Care | $51.3M | 7 |
| HEALTH AND WELFARE FUND OF THE PBA OF THE CITY OF NEW YORK | New York | — | $51.2M | 6 |
| JASA CORPORATION | New York | S50Z Community Improvement & Capacity Building | $51M | 2 |
| UNION COMMUNITY HEALTH CENTER INC | Bronx | F300 Mental Health & Crisis Intervention | $50.8M | 9 |
| FEDERATION OF JEWISH COMMUNITIES OF THE CIS INC | New York | T31J Philanthropy, Voluntarism & Grantmaking Foundations | $50.8M | 0 |
| CENTRAL NEW YORK COMMUNITY FOUNDATION INC | Syracuse | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $50.7M | 8 |
| Science Academies of New York Charter Schools | Syracuse | — | $50.7M | 5 |
| HADASSAH MEDICAL RELIEF ASSOCIATION INC | New York | Q30 International, Foreign Affairs & National Security | $50.6M | 3 |
| VITAL PROJECTS FUND INC | New York | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $50.3M | 3 |
| Stony Brook Radiology University Faculty Practice Corporation | Stony Brook | E20 Health Care | $50.2M | 9 |
| FACULTY-STUDENT ASSOCIATION OF STATE UNIVERSITY OF NEW YORK AT BUFFALO INC | Buffalo | B43I Education | $50.1M | 6 |
| NYSARC INC CATTARAUGUS NIAGARA COUNTIES CHAPTER | Olean | — | $50M | 0 |
| OUR LADY OF CONSOLATION GERIATRIC CARE CENTER | West Islip | E910 Health Care | $50M | 9 |
| JERICHO ROAD MINISTRIES INC | Buffalo | X21 Religion-Related | $50M | 9 |
| LOCAL 812 HEALTH FUND | Great Neck | — | $50M | 5 |
| TIEGERMAN SCHOOL | Glen Cove | — | $50M | 8 |
| PUBLIC PREP CHARTER SCHOOL ACADEMIES | Bronx | B29 Education | $49.8M | 7 |
| McAuley Seton Home Care | Buffalo | E99I Health Care | $49.8M | 24 |
| ACADEMIC MEDICINE SERVICES INC | Amherst | E31 Health Care | $49.8M | 15 |
| AMERICAN INSTITUTE OF CHEMICAL ENGINEERS | New York | — | $49.7M | 14 |
| Montefiore Einstein Advanced Care | Tarrytown | E30 Health Care | $49.6M | 9 |
| FOUNTAIN HOUSE INC | New York | F330 Mental Health & Crisis Intervention | $49.4M | 11 |
| ADAPT OF THE HUDSON VALLEY INC | Middletown | P70 Human Services | $49.1M | 7 |
| ATTWOOD WILLIAMS CHARITABLE FOUNDATION | Bedford Hills | T50 Philanthropy, Voluntarism & Grantmaking Foundations | $49M | 0 |
| EVERCARE CHOICE INC | Newburgh | E60I Health Care | $48.9M | 10 |
| LASANTE HEALTH CENTER | Brooklyn | E32 Health Care | $48.9M | 7 |
| UNIVERSITY PHYSICIANS OF BROOKLYN INC | Brooklyn | B54Z Education | $48.9M | 22 |
| WORKING FAMILIES ORGANIZATION INC | Brooklyn | R40 Civil Rights, Social Action & Advocacy | $48.8M | 0 |
| COMMUNITY FOUNDATION FOR GREATER BUFFALO INC | Buffalo | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $48.7M | 8 |
| THE CORNING MUSEUM OF GLASS | Corning | — | $48.6M | 8 |
| LAKEVILLE SURGERY PC DBA NORTH SHORE-LIJ SURGERY | Westbury | E60 Health Care | $48.6M | 5 |
| PEPITA FOUNDATION INC C/O RCM | New York | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $48.5M | 0 |
| RICHMOND CHILDREN CENTER INC | Mount Kisco | F320 Mental Health & Crisis Intervention | $48.5M | 7 |
| ROCHESTER REGIONAL HEALTH | Rochester | E21 Health Care | $48.4M | 5 |
| FOUNDATION FOR THE GLOBAL COMPACT | New York | Q42 International, Foreign Affairs & National Security | $48.3M | 7 |
| UNITED TEAMSTER FUND | Brooklyn | — | $48.3M | 8 |
