| LUTHERAN SOCIAL SERVICES OF METROPOLITAN | New York | — | $48.6M | 7 |
| UNITED TEAMSTER FUND | Brooklyn | — | $48.3M | 8 |
| MERCYFIRST | Syosset | P320 Human Services | $48.3M | 8 |
| L'REFUAH MEDICAL AND REHABILITATION CENTER | Brooklyn | E30 Health Care | $48.2M | 8 |
| INTERNATIONAL UNION OF OPERATING | Farmingdale | — | $48.2M | 13 |
| SERVICE EMPLOYEES BENEFIT FUND | Syracuse | Y43 Mutual & Membership Benefit | $48.2M | 1 |
| BOB WOODRUFF FAMILY FOUNDATION INC | Long Island City | E99 Health Care | $48.1M | 7 |
| CATHOLIC CHARITIES OF THE DIOCESE OF | Albany | — | $48.1M | 10 |
| SUSTAINABLE MARKETS FOUNDATION | New York | C30 Environment | $48M | 0 |
| AMERICAN FOUNDATION FOR SUICIDE | New York | F110 Mental Health & Crisis Intervention | $48M | 11 |
| GOOD SHEPHERD HOSPICE | Farmingdale | — | $48M | 7 |
| ROCHESTER AREA COMMUNITY FOUNDATION | Rochester | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $47.9M | 7 |
| ALL MY CHILDREN DAYCARE & | Jamaica Estates | P33 Human Services | $47.8M | 6 |
| ALLEGANY-STEUBEN COUNTIES CHAPTER | Wellsville | P820 Human Services | $47.7M | 6 |
| POSTGRADUATE CENTER FOR MENTAL HEALTH | New York | — | $47.6M | 8 |
| FRANZISKA RACKER CENTERS INC | Ithaca | P820 Human Services | $47.6M | 6 |
| Steamfitters Industry Security Benefit Fund | Long Island City | J40 Employment | $47.5M | 9 |
| COMIC RELIEF INC DBA COMIC RELIEF US | New York | T12 Philanthropy, Voluntarism & Grantmaking Foundations | $47.5M | 6 |
| NEW YORK LEGAL ASSISTANCE GROUP INC | New York | — | $47.4M | 11 |
| TREVOR DAY SCHOOL | New York | B200 Education | $47.4M | 10 |
| LANTERN COMMUNITY SERVICES INC | New York | L21 Housing & Shelter | $47.3M | 8 |
| ESSEX COUNTY CHAPTER NYSARC | Port Henry | P820 Human Services | $47.2M | 10 |
| ST NICKS ALLIANCE CORPORATION | Brooklyn | S200 Community Improvement & Capacity Building | $47.2M | 8 |
| THE STUDIO MUSEUM IN HARLEM | New York | A500 Arts, Culture & Humanities | $47.2M | 8 |
| EDEN II SCHOOL FOR AUTISTIC CHILDREN | Staten Island | B280 Education | $47.2M | 7 |
| SCHUYLER HOSPITAL INC | Montour Falls | E22Z Health Care | $47.2M | 8 |
| KIPP CAPITAL REGION PUBLIC CHARTER | Albany | B29 Education | $47.2M | 8 |
| BASSETT HOSPITAL OF SCHOHARIE COUNTY | Cobleskill | E220 Health Care | $47.1M | 10 |
| WESTCHESTER COUNTRY CLUB | Rye | N50 Recreation & Sports | $47.1M | 9 |
| The Frick Collection | New York | — | $47M | 14 |
| INTEGRATION CHARTER SCHOOLS | State Island | B25 Education | $47M | 15 |
| SOROS ECONOMIC DEVELOPMENT FUND | New York | T23 Philanthropy, Voluntarism & Grantmaking Foundations | $46.9M | 0 |
| ANDERSON CENTER FOR AUTISM | Staatsburg | B20Z Education | $46.7M | 8 |
| GIVE LIVELY FOUNDATION INC | New York | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $46.7M | 0 |
| NATIONAL KIDNEY FOUNDATION INC | New York | G44Z Diseases, Disorders & Medical Disciplines | $46.7M | 13 |
| LOCAL 580 - INSURANCE FUND | New York | — | $46.7M | 5 |
| Hudson Valley Hospice Inc | Poughkeepsie | P74Z Human Services | $46.7M | 9 |
| COMMUNITY SERVICES FOR EVERY1 INC | Buffalo | P820 Human Services | $46.6M | 5 |
| UNION THEOLOGICAL SEMINARY | New York | B500 Education | $46.6M | 11 |
| BROOKLYN ACADEMY OF MUSIC INC | Brooklyn | — | $46.6M | 13 |
| ACADEMIC MEDICINE SERVICES INC | Amherst | E31 Health Care | $46.5M | 14 |
| VNS HEALTH BEHAVIORAL HEALTH INC | New York | F32 Mental Health & Crisis Intervention | $46.4M | 10 |
| JOSEPH P ADDABBO FAMILY HEALTH CENTER | Arverne | — | $46.4M | 9 |
| ICNA RELIEF USA PROGRAMS INC | New Hyde Park | M20 Public Safety, Disaster Preparedness & Relief | $46.4M | 2 |
| TRUSTEES OF MASONIC HALL AND ASYLUM FUND | New York | P750 Human Services | $46.4M | 5 |
| UNITED WIRE METAL & MACHINE HEALTH & WE | Long Island City | — | $46.3M | 11 |
| BEHAVIORAL HEALTH SERVICES NORTH INC | Plattsburgh | F320 Mental Health & Crisis Intervention | $46.3M | 8 |
| THE NIGHTINGALE-BAMFORD SCHOOL | New York | B200 Education | $46.2M | 8 |
| RICHMOND CHILDREN CENTER INC | Mount Kisco | F320 Mental Health & Crisis Intervention | $46.2M | 7 |
| UNITED CEREBRAL PALSY ASSOCIATION OF NASSAU COUNTY INC | Roosevelt | G200 Diseases, Disorders & Medical Disciplines | $46.1M | 9 |
