| MASS GENERAL BRIGHAM INCORPORATED & | Somerville | E21 Health Care | $23.5B | 261 |
| FIDELITY INVESTMENTS CHARITABLE GIFT FUND | Boston | S500 Community Improvement & Capacity Building | $14.7B | 0 |
| President and Fellows of Harvard College | Cambridge | B500 Education | $6.1B | 25 |
| BLUE CROSS BLUE SHIELD OF MASSACHUSETTS HMO BLUE INC | Boston | — | $5.9B | 17 |
| BOSTON MEDICAL CENTER HEALTH PLAN INC | Charlestown | E110 Health Care | $5.2B | 13 |
| Massachusetts Institute of Technology | Cambridge | B430 Education | $5B | 20 |
| UMass Memorial Health Care Inc & Affiliates | Worcester | E210 Health Care | $4.2B | 72 |
| Dana-Farber Cancer Institute Inc | Boston | H300 Medical Research | $3.5B | 19 |
| Trustees of Boston University | Boston | — | $3.3B | 13 |
| Children's Hospital Corporation | Boston | E240 Health Care | $3.2B | 27 |
| BETH ISRAEL DEACONESS MEDICAL CENTER | Charlestown | E220 Health Care | $3.1B | 28 |
| TUFTS ASSOCIATED HEALTH MAINTENANCE | Canton | — | $2.7B | 15 |
| BOSTON MEDICAL CENTER CORPORATION | Boston | E22Z Health Care | $2.6B | 17 |
| NORTHEASTERN UNIVERSITY | Boston | B83 Education | $2.6B | 23 |
| COMMONWEALTH CARE ALLIANCE INC | Boston | E310 Health Care | $2.6B | 31 |
| TUFTS HEALTH PUBLIC PLANS INC | Canton | Y20 Mutual & Membership Benefit | $2.5B | 15 |
| HARVARD PILGRIM HEALTH CARE INC | Canton | E310 Health Care | $2.2B | 29 |
| Harvard Management Private Equity Corp | Boston | B110 Education | $2B | 3 |
| TEMPUS UNLIMITED INC | Stoughton | G23Z Diseases, Disorders & Medical Disciplines | $2B | 5 |
| MASS GENERAL BRIGHAM INCORPORATED | Somerville | E21 Health Care | $1.9B | 22 |
| BAYSTATE MEDICAL CENTER INC | Springfield | E220 Health Care | $1.8B | 18 |
| MASS GENERAL BRIGHAM HEALTH PLAN INC | Somerville | E81Z Health Care | $1.8B | 24 |
| FALLON COMMUNITY HEALTH PLAN INC | Worcester | E800 Health Care | $1.8B | 25 |
| BANK OF AMERICA CHARITABLE GIFT FD | Boston | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $1.7B | 1 |
| TRUSTEES OF BOSTON COLLEGE | Chestnut Hill | B430 Education | $1.6B | 19 |
| TUFTS MEDICAL CENTER GROUP RETURN | Boston | — | $1.4B | 22 |
| Trustees of Tufts College | Medford | B430 Education | $1.3B | 29 |
| FIDELITY FOUNDATION | Boston | — | $1.3B | 3 |
| NORTHROP GRUMMAN CORPORATION VEBA | Quincy | Y20Z Mutual & Membership Benefit | $1.3B | 1 |
| LAHEY CLINIC HOSPITAL INC | Charlestown | E220 Health Care | $1.2B | 39 |
| SOUTHCOAST HOSPITALS GROUP INC | New Bedford | E200 Health Care | $1.2B | 20 |
| CAPE COD HEALTHCARE INC & AFFILIATES | Hyannis | E210 Health Care | $1.1B | 29 |
| JOHNSON & JOHNSON VEBA TRUST | Quincy | — | $1.1B | 1 |
| HEALTH NEW ENGLAND INC | Springfield | E310 Health Care | $990.8M | 17 |
| SOUTH SHORE HOSPITAL INC | South Weymouth | E220 Health Care | $895.9M | 21 |
| MGB POOLED HOLDINGS LLC | Somerville | E19 Health Care | $892.1M | 5 |
| BETH ISRAEL LAHEY HEALTH INC | Charlestown | E19 Health Care | $876.1M | 39 |
| CHARLES STARK DRAPER LABORATORY INC | Cambridge | U400 Science & Technology | $871.1M | 24 |
| THE BROAD INSTITUTE INC | Cambridge | H20 Medical Research | $860.4M | 13 |
| BILL & MELINDA GATES MEDICAL RESEARCH | Cambridge | E05 Health Care | $809.5M | 18 |
| BERKSHIRE MEDICAL CENTER INC | Pittsfield | E220 Health Care | $757.5M | 15 |
| HARVARD MEDICAL FACULTY PHYSICIANS AT | Boston | E300 Health Care | $714.6M | 29 |
| UMass Memorial Health Care Inc (Parent) | Worcester | E110 Health Care | $692.5M | 37 |
| THE LOWELL GENERAL HOSPITAL | Lowell | E220 Health Care | $605.3M | 13 |
| THE TRUSTEES OF THE SMITH COLLEGE | Northampton | B430 Education | $587.5M | 19 |
| NORTHEAST HOSPITAL CORPORATION | Charlestown | E220 Health Care | $537.4M | 26 |
| HARVARD PILGRIM HEALTH CARE OF NEW ENGLAND | Canton | E81Z Health Care | $502M | 6 |
| BRANDEIS UNIVERSITY | Waltham | B430 Education | $478.7M | 21 |
| WORCESTER POLYTECHNIC INSTITUTE | Worcester | — | $462.7M | 15 |
| Children's Hospital Pediatric | Boston | E31 Health Care | $454.9M | 27 |
