| UPPER VALLEY MEDICAL CENTER | Dayton | E220 Health Care | $163.5M | 32 |
| GOSPEL LIGHT MENNONITE CHURCH | Canton | X20 Religion-Related | $163.4M | 12 |
| OHIO CARPENTERS' HEALTH FUND | Columbus | Y43 Mutual & Membership Benefit | $158.4M | 7 |
| TRIHEALTH PHYSICIAN INSTITUTE | Cincinnati | E112 Health Care | $158.1M | 17 |
| EMMA L BIXBY MEDICAL CENTER | Toledo | E220 Health Care | $155.8M | 15 |
| FRANCISCAN UNIVERSITY OF STEUBENVILLE | Steubenville | B430 Education | $155.4M | 15 |
| Ohio Northern University | Ada | B420 Education | $154.2M | 10 |
| The University of Findlay | Findlay | B420 Education | $152.5M | 11 |
| THE UNIVERSITY OF CINCINNATI FOUNDATION | Cincinnati | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $148.3M | 13 |
| BALDWIN WALLACE UNIVERSITY | Berea | B430 Education | $147M | 12 |
| BATTELLE VOLUNTARY EMPLOYEES' | Columbus | — | $143.6M | 4 |
| MARY RUTAN HOSPITAL | Bellefontaine | E220 Health Care | $139.6M | 12 |
| Alliance Physicians Inc | Miamisburg | E300 Health Care | $136.3M | 20 |
| WOOD COUNTY HOSPITAL ASSOCIATION | Bowling Green | E220 Health Care | $133.4M | 9 |
| ASHLAND UNIVERSITY | Ashland | B430 Education | $132.3M | 11 |
| Honda Health and Welfare Benefits Trust | Marysville | — | $132.3M | 0 |
| 37 KEMBA FINANCIAL CREDIT UNION | Gahanna | — | $130.5M | 16 |
| THE WEXNER FAMILY CHARITABLE FUND | New Albany | — | $129.9M | 0 |
| Council on Aging of Southwestern Ohio | Blue Ash | P81Z Human Services | $129M | 11 |
| OHIO LIVING COMMUNITIES | Westerville | P750 Human Services | $128.8M | 11 |
| OHIO VIRTUAL ACADEMY | Maumee | B200 Education | $128.7M | 7 |
| UNITED FOOD & COMMERCIAL WORKERS | Broadview Heights | — | $127.8M | 0 |
| WESTERN RESERVE AREA AGENCY ON AGING | Cleveland | P810 Human Services | $127.3M | 19 |
| HOLZER HEALTH SYSTEM | Gallipolis | E210 Health Care | $126.8M | 32 |
| COMPUNET CLINICAL LABORATORIES LLC | Dayton | — | $125.5M | 12 |
| CAPITAL UNIVERSITY | Columbus | B430 Education | $125.1M | 13 |
| FULTON COUNTY HEALTH CENTER | Wauseon | E220 Health Care | $125.1M | 12 |
| BAY PARK COMMUNITY HOSPITAL | Toledo | E220 Health Care | $123.6M | 14 |
| NESTLE IN THE USA RETIREE WELFARE | Solon | — | $123.4M | 1 |
| Mid-Ohio Foodbank | Grove City | — | $121.8M | 8 |
| THE SHELBY COUNTY MEMORIAL HOSPITAL | Sidney | E220 Health Care | $121.2M | 11 |
| GREATER CLEVELAND FOOD BANK INC | Cleveland | K31Z Food, Agriculture & Nutrition | $116.5M | 13 |
| NATIONAL CHURCH RESIDENCES | Columbus | L210 Housing & Shelter | $116.1M | 17 |
| UNIVERSITY OF TOLEDO PHYSICIANS CLINICAL | Toledo | — | $114.8M | 49 |
| First Catholic Slovak Union of the United States of America and Canada | Independence | — | $114.4M | 20 |
| SALEM COMMUNITY HOSPITAL | Salem | E220 Health Care | $113.5M | 13 |
| JEWISH FEDERATION OF CLEVELAND | Cleveland | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $113.3M | 14 |
| OHIO WESLEYAN UNIVERSITY | Delaware | B420 Education | $112.1M | 11 |
| UNITED CHURCH HOMES INC | Marion | P750 Human Services | $111.4M | 12 |
| THE ELIZABETH GAMBLE DEACONESS HOME | Cincinnati | E12 Health Care | $110M | 2 |
| SIGNATURE HEALTH INC | Kirtland | F30 Mental Health & Crisis Intervention | $109M | 12 |
| GOODWILL EASTER SEALS MIAMI VALLEY | Dayton | J32Z Employment | $108.4M | 10 |
| JOINT TOWNSHIP DISTRICT | St Marys | E220 Health Care | $107.7M | 14 |
| Otterbein University | Westerville | B430 Education | $106.5M | 12 |
| THE PROCTER & GAMBLE BENEFIT PLAN | Cincinnati | — | $106.4M | 0 |
| JACK JOSEPH AND MORTON MANDEL | Cleveland | T110 Philanthropy, Voluntarism & Grantmaking Foundations | $105.1M | 8 |
| DEFIANCE HOSPITAL INC | Toledo | E220 Health Care | $103.9M | 14 |
| CareSource West Virginia Co | Dayton | E31 Health Care | $103.3M | 7 |
| Cleveland Museum of Art | Cleveland | A510 Arts, Culture & Humanities | $102.9M | 14 |
| I AM BOUNDLESS INC | Worthington | P82 Human Services | $100.4M | 10 |
