| ORIANA HOUSE INC | Akron | I440 Crime & Legal-Related | $72.4M | 16 |
| COLEMAN PROFESSIONAL SERVICES INC | Kent | F300 Mental Health & Crisis Intervention | $72M | 12 |
| FIRST CATHOLIC SLOVAK LADIES ASSOCIATION | Cleveland | — | $71M | 19 |
| Marietta College | Marietta | B420 Education | $70.5M | 10 |
| The Ohio University Foundation | Athens | B110 Education | $69.7M | 20 |
| Walsh University Inc | North Canton | B430 Education | $69.7M | 8 |
| ZOOLOGICAL SOCIETY OF CINCINNATI | Cincinnati | D500 Animal-Related | $69.4M | 11 |
| CINCINNATI CENTER CITY DEVELOPMENT CORP | Cincinnati | S31 Community Improvement & Capacity Building | $69.2M | 8 |
| OHIO EDUCATION ASSOCIATION | Columbus | — | $67.8M | 12 |
| KAPPA KAPPA GAMMA FRATERNITY - GROUP | Dublin | — | $67.6M | 0 |
| CHI LIVING COMMUNITIES | Oregon | P190 Human Services | $67.5M | 10 |
| UNION RURAL ELECTRIC CO-OP INC | Marysville | — | $66.9M | 15 |
| TRANSPORTATION RESEARCH CENTER INC | East Liberty | W050 Public & Societal Benefit | $66.7M | 13 |
| MADISON COUNTY COMMUNITY HOSPITAL | London | E220 Health Care | $66.3M | 9 |
| GRACEWORKS LUTHERAN SERVICES | Dayton | P200 Human Services | $66.2M | 9 |
| WHOLESALERS' GROUP LIFE AND | Dayton | — | $66.1M | 1 |
| MUSKINGUM UNIVERSITY | New Concord | B410 Education | $65.9M | 18 |
| Mount Vernon Nazarene University | Mount Vernon | B430 Education | $65.9M | 5 |
| AKRON-CANTON REGIONAL FOODBANK | Akron | K31Z Food, Agriculture & Nutrition | $65.7M | 7 |
| YOUNG MENS CHRISTIAN ASSOCIATION OF CENTRAL OHIO | Columbus | P27Z Human Services | $65.4M | 8 |
| THE ROCK AND ROLL HALL OF FAME | Cleveland | A500 Arts, Culture & Humanities | $65.3M | 14 |
| Blanchard Valley Health System | Findlay | E210 Health Care | $65.3M | 9 |
| MOUNT ST JOSEPH UNIVERSITY | Cincinnati | B420 Education | $65.2M | 9 |
| H B MAGRUDER MEMORIAL HOSPITAL | Port Clinton | E220 Health Care | $65M | 9 |
| HEIDELBERG UNIVERSITY | Tiffin | B430 Education | $64.6M | 7 |
| ADENA FAYETTE MEDICAL CENTER | Chillicothe | E22 Health Care | $63.7M | 10 |
| HOSPICE OF CINCINNATI INCORPORATED | Cincinnati | — | $63.7M | 10 |
| DIRECTION HOME AKRON CANTON AREA AGENCY | Uniontown | P81Z Human Services | $63.2M | 8 |
| GOODWILL INDUSTRIES OF CENTRAL OHIO INC | Columbus | J320 Employment | $63.1M | 9 |
| CONSOLIDATED COOPERATIVE | Mt Gilead | — | $62.8M | 21 |
| DEL-CO WATER COMPANY INC | Delaware | K20 Food, Agriculture & Nutrition | $62.3M | 17 |
| ASSOCIATES HEALTH TRUST | Solon | — | $61.8M | 0 |
| Muskingum Valley Health Center | Zanesville | E21 Health Care | $61.7M | 9 |
| THE VILLAGE NETWORK | Wooster | I30Z Crime & Legal-Related | $60.5M | 6 |
| DELTA GAMMA FRATERNITY | Columbus | — | $60.4M | 0 |
| The Western and Southern Life Insurance | Cincinnati | — | $60.1M | 0 |
| CATHOLIC CHARITIES CORPORATION | Cleveland | P200 Human Services | $60M | 10 |
| IDEASTREAM | Cleveland | O50 Youth Development | $59.9M | 12 |
| HAWKEN SCHOOL | Gates Mills | B200 Education | $59.7M | 15 |
| McGregor Pace | Cleveland | P75 Human Services | $59.7M | 9 |
| THE DAYTON FOUNDATION | Dayton | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $59.3M | 8 |
| JOHNSON CHARITABLE GIFT FUND | Cincinnati | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $58.9M | 0 |
| GREAT LAKES COMMUNITY ACTION PARTNERSHIP | Fremont | P200 Human Services | $58.8M | 5 |
| THE BUCKEYE RANCH INC | Whitehall | P730 Human Services | $58.4M | 10 |
| CUYAHOGA COUNTY CONVENTION | Cleveland | A20 Arts, Culture & Humanities | $58.3M | 1 |
| COMMUNITY SHELTER BOARD | Columbus | — | $57.9M | 8 |
| CHN HOUSING PARTNERS | Cleveland | L800 Housing & Shelter | $57.5M | 11 |
| THE OAK CLINIC | Uniontown | G500 Diseases, Disorders & Medical Disciplines | $57.1M | 4 |
| COMMUNITY HEALTH ASSOCIATES INC | Galion | E32Z Health Care | $56.9M | 7 |
| OHIO DISTRICT 5 AREA AGENCY ON AGING | Ontario | — | $56.6M | 4 |
