| ALABAMA TEACHERS CREDIT UNION | Gadsden | — | $28.3M | 9 |
| BAPTIST HEALTH SYSTEM INC | Birmingham | E210 Health Care | $28.3M | 3 |
| SOUTHEAST ALABAMA RURAL HEALTH ASSOCIATES | Troy | E30 Health Care | $27.7M | 8 |
| THE LEARNING TREE INC | Mobile | — | $27.7M | 2 |
| WHATLEY HEALTH SERVICES INC | Tuscaloosa | — | $27.1M | 9 |
| Alabama Forest Land Trust Inc | Mobile | — | $27M | 1 |
| BAYSIDE ACADEMY INC | Daphne | B24Z Education | $26.9M | 1 |
| WINSOUTH CREDIT UNION | Gadsden | — | $26.7M | 9 |
| COUNTRY CLUB OF BIRMINGHAM | Mountain Brook | N50 Recreation & Sports | $26.1M | 7 |
| NORTHWEST AL MENTAL HEALTH CENTER | Jasper | F32Z Mental Health & Crisis Intervention | $26.1M | 5 |
| COMMUNITY SERVICE PROGRAMS OF WEST ALABAMA INC | Tuscaloosa | P200 Human Services | $25.7M | 3 |
| SCHOLARSHIPS FOR KIDS INC | Birmingham | B82 Education | $24.9M | 2 |
| MONTG COMM ACTION COMMITTEE & COMMUNITY DEV CORP | Montgomery | P200 Human Services | $24.7M | 0 |
| Redstone Village FKA REDSTONE MILITARY RETIREMENT | Huntsville | L22 Housing & Shelter | $24.6M | 5 |
| THE DOUBLE OAKS FOUNDATION | Birmingham | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $24.3M | 0 |
| COMMUNITY ACTION AGENCY OF NORTHEAST ALABAMA INC | Rainsville | P20Z Human Services | $24M | 1 |
| BIG OAK RANCH INC | Gadsden | P730 Human Services | $23.9M | 8 |
| BARBER VINTAGE MOTORSPORTS MUSEUM | Birmingham | A50Z Arts, Culture & Humanities | $23.6M | 2 |
| ERC INC VOLUNTARY EMPLOYEE BENEFICIARY ASSOCIATION PLAN | Huntsville | Y43 Mutual & Membership Benefit | $23.4M | 0 |
| UMS-WRIGHT CORPORATION | Mobile | — | $23.2M | 5 |
| Community Foundation of Greater Huntsville | Huntsville | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $23.1M | 1 |
| EASTER SEALS ALABAMA INC | Montgomery | E50Z Health Care | $22.9M | 8 |
| Christ Health Center Inc | Birmingham | E32 Health Care | $22.4M | 13 |
| ALABAMA GOODWILL INDUSTRIES INC | Birmingham | — | $22.4M | 4 |
| US SPACE & ROCKET CENTER EDUCATION FOUNDATION | Huntsville | P20 Human Services | $22.2M | 1 |
| MIZELL MEMORIAL HOSPITAL INC | Opp | E220 Health Care | $22.1M | 10 |
| THOMPSON TRACTOR COMPANY INC EMPLOYEE BENEFIT TRUST | Birmingham | — | $21.7M | 0 |
| Stillman College | Tuscaloosa | B420 Education | $21.4M | 6 |
| YOUNG MENS CHRISTIAN ASSOCIATION | Montgomery | P27Z Human Services | $21M | 13 |
| MARSHALL-DEKALB ELECTRIC COOPERATIVE | Boaz | — | $20.4M | 11 |
| SOUTHWESTERN ATHLETIC CONFERENCE | Birmingham | — | $20M | 1 |
| Second Wind Programs Inc | Birmingham | Z99Z Unknown | $19.8M | 6 |
| MERCY LIFE OF ALABAMA | Mobile | E30 Health Care | $19.7M | 11 |
| CENTRAL ALABAMA COMMUNITY FOUNDATION INC | Montgomery | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $19.7M | 2 |
| COMMUNITY FOUNDATION OF NORTHEAST ALABAMA | Anniston | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $19.6M | 5 |
| COMMUNITY HOSPITAL INC | Tallassee | — | $19.4M | 7 |
| ESCAMBIA COUNTY ALABAMA COMMUNITY HOSPITALS INC | Atmore | E220 Health Care | $19.4M | 8 |
| ALABAMA EDUCATION ASSOCIATION | Montgomery | — | $19.4M | 9 |
| ALABAMA PARTNERSHIP FOR CHILDREN | Montgomery | B60 Education | $19.1M | 1 |
| J L BEDSOLE FOUNDATION REGIONS BANK CO-TRUSTEE | Mobile | T20Z Philanthropy, Voluntarism & Grantmaking Foundations | $19.1M | 5 |
| ST PAUL'S EPISCOPAL SCHOOL | Mobile | B200 Education | $19M | 1 |
| AFOF L - AGC BUILDING TRADES WELFARE PLAN | Mobile | — | $19M | 0 |
| AIDS ALABAMA INC | Birmingham | G81 Diseases, Disorders & Medical Disciplines | $18.8M | 4 |
| THE HEALTH CARE AUTHORITY OF THE CITY OF ENTERPRISE INC | Enterprise | E91Z Health Care | $18.5M | 5 |
| TVA COMMUNITY CREDIT UNION | Muscle Shoals | — | $18.4M | 2 |
| LIFELINE CHILDREN'S SERVICES INC | Birmingham | X20 Religion-Related | $18.2M | 0 |
| EKVNV YEFOLECVLKE | Weogufka | P80 Human Services | $18.2M | 0 |
| THE JACKSONVILLE STATE UNIVERSITY FOUNDATION INC | Jacksonville | B110 Education | $18.1M | 0 |
| Lakeland Community Hospital Inc | Haleyville | E20 Health Care | $18M | 9 |
| UNIVERSITY OF ALABAMA ALUMNI ASSOCIATION | Tuscaloosa | B84 Education | $17.9M | 7 |
