| Vanderbilt University Medical Center | Nashville | E20 Health Care | $6.9B | 20 |
| AMERICAN LEBANESE SYRIAN ASSOCIATED | Memphis | X12 Religion-Related | $2.7B | 13 |
| Vanderbilt University | Nashville | B43 Education | $2.3B | 18 |
| Methodist Healthcare-Memphis Hospitals | Memphis | E200 Health Care | $1.9B | 23 |
| ST JUDE CHILDREN'S RESEARCH HOSPITAL | Memphis | E240 Health Care | $1.7B | 13 |
| UNIVERSITY HEALTH SYSTEM INC | Knoxville | E220 Health Care | $1.5B | 19 |
| BAPTIST MEMORIAL HOSPITAL | Memphis | E220 Health Care | $975.5M | 26 |
| Mountain States Health Alliance | Johnson City | E220 Health Care | $974.9M | 24 |
| MIDDLE TENNESSEE ELECTRIC | Murfreesboro | — | $897.3M | 27 |
| Wellmont Health System | Johnson City | E210 Health Care | $840.4M | 22 |
| DIALYSIS CLINIC INC | Nashville | E300 Health Care | $736.4M | 14 |
| SHELBY COUNTY HEALTH CARE CORPORATION | Memphis | E05 Health Care | $736.1M | 7 |
| CHSCOMMUNITY HEALTH SYSTEMS INC VEBA | Franklin | Y43 Mutual & Membership Benefit | $680.1M | 0 |
| THE BILL GATTON FOUNDATION | Bristol | — | $664.2M | 3 |
| MEMORIAL HEALTH CARE SYSTEM INC | Chattanooga | — | $655.7M | 17 |
| Belmont University | Nashville | B430 Education | $523.2M | 29 |
| Parkwest Medical Center | Knoxville | E22 Health Care | $469.8M | 13 |
| EASTMAN CREDIT UNION | Kingsport | — | $436.1M | 16 |
| YOUTH VILAGES INC | Memphis | P300 Human Services | $411.6M | 29 |
| BAPTIST MEMORIAL HOSPITAL-TIPTON INC | Covington | E220 Health Care | $407.1M | 16 |
| CUMBERLAND ELECTRIC | Clarksville | — | $404.7M | 22 |
| Fort Sanders Regional Medical Center | Knoxville | E20 Health Care | $399.3M | 12 |
| EAST TENNESSEE CHILDREN'S HOSPITAL | Knoxville | E240 Health Care | $365.4M | 20 |
| BAPTIST MEMORIAL HEALTH CARE CORPORATION | Memphis | E210 Health Care | $351.2M | 55 |
| DUCKS UNLIMITED INC | Memphis | C32Z Environment | $302.2M | 14 |
| BAPTIST MEMORIAL HOSPITAL- | Memphis | E22 Health Care | $288.3M | 23 |
| VOLUNTEER ENERGY COOPERATIVE | Decatur | — | $282.1M | 21 |
| NISSAN NORTH AMERICA INC VOLUNTARY | Franklin | — | $258.4M | 1 |
| LIPSCOMB UNIVERSITY | Nashville | — | $242.5M | 11 |
| BAPTIST MEMORIAL MEDICAL GROUP INC | Memphis | E32Z Health Care | $241M | 31 |
| DELTA DENTAL OF TENNESSEE INC | Nashville | — | $240.3M | 24 |
| KNOXVILLE TVA EMPLOYEES CREDIT UNION | Knoxville | — | $239.9M | 41 |
| DUCK RIVER ELECTRIC MEMBERSHIP CORP | Shelbyville | — | $239.1M | 23 |
| Covenant Health | Knoxville | E210 Health Care | $235.7M | 30 |
| LINCOLN MEMORIAL UNIVERSITY | Harrogate | B430 Education | $225.1M | 66 |
| BAPTIST MEMORIAL MEDICAL MINISTRIES | Memphis | — | $217.7M | 7 |
| THE UNIVERSITY OF TENNESSEE | Knoxville | B11 Education | $211.8M | 26 |
| Methodist Le Bonheur Healthcare Inc | Memphis | E210 Health Care | $209.8M | 18 |
| MEHARRY MEDICAL COLLEGE | Nashville | B500 Education | $209.4M | 19 |
| SOUTHERN DISTRICT UBC | Goodletsville | J40 Employment | $205.6M | 0 |
| Johnston Memorial Hospital Inc | Johnson City | E220 Health Care | $197.1M | 17 |
| Rhodes College | Memphis | B420 Education | $187.6M | 16 |
| Methodist Medical Center | Knoxville | E220 Health Care | $184.4M | 14 |
| UNIVERSITY OF THE SOUTH | Sewanee | B99 Education | $175.3M | 20 |
| THE COMMUNITY FOUNDATION OF MIDDLE | Nashville | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $169.7M | 8 |
| LIFE CARE CENTERS OF AMERICA INC | Cleveland | Y43 Mutual & Membership Benefit | $163.4M | 0 |
| THE GUARDIAN FOUNDATION INC-AFFILIATES | Ooltewah | E910 Health Care | $162.3M | 8 |
| LeConte Medical Center | Knoxville | E220 Health Care | $160.4M | 10 |
| EDUCATION LOAN FINANCE INC | Knoxville | B82 Education | $152.8M | 19 |
| VOLUNTEER CORPORATE CREDIT UNION | Nashville | — | $150.2M | 10 |
