| STEPHENS COLLEGE | Columbia | B420 Education | $23.7M | 12 |
| Rockhill Orthopaedic Specialists Inc | Kansas City | E21 Health Care | $23.7M | 4 |
| SENIORAGE AREA AGENCY ON AGING | Springfield | P81Z Human Services | $23.6M | 8 |
| SAINT FRANCIS HEALTHCARE REAL ESTATE | Cape Girardeau | E11 Health Care | $23.6M | 1 |
| SOCIETY OF ST VINCENT DE PAUL ARCHDIOCESAN COUNCIL OF ST LOUIS | St Louis | P200 Human Services | $23.5M | 1 |
| Woodhaven Learning Center d/b/a Woodhaven | Columbia | — | $23.4M | 8 |
| THE MARVIN M SCHWAN CHARITABLE FOUNDATION | Earth City | X120 Religion-Related | $23.4M | 5 |
| URBAN STRATEGIES INC | Saint Louis | P80 Human Services | $23.4M | 8 |
| FOSTERADOPT CONNECT INC | Independence | P32 Human Services | $23.2M | 6 |
| STARLIGHT THEATRE ASSOCIATION OF KANSAS CITY INC | Kansas City | A610 Arts, Culture & Humanities | $23.2M | 7 |
| THE BARSTOW SCHOOL | Kansas City | — | $23.1M | 6 |
| MISSOURI LIFE AND HEALTH INSURANCE GUARANTY ASSOCIATION | Jefferson City | — | $23.1M | 2 |
| GENERAL JOHN J PERSHING MEMORIAL HOSPITAL ASSOCIATION | Brookfield | E22Z Health Care | $22.9M | 7 |
| HERMANN FAMILY FOUNDATION | St Louis | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $22.8M | 0 |
| KATE B REYNOLDS CHARITABLE TRUST | Saint Louis | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $22.8M | 1 |
| KUMMER MISSOURI S&T FOUNDATION | Rolla | B11 Education | $22.7M | 1 |
| KANSAS CITY SYMPHONY | Kansas City | A690 Arts, Culture & Humanities | $22.7M | 12 |
| MERCY ST FRANCIS HOSPITAL | Mountain View | E220 Health Care | $22.5M | 13 |
| WILLOWS WAY INC | St Charles | P820 Human Services | $22.5M | 3 |
| EVERGY INC JOINT TRUSTEED HEALTH & WELFARE FUND | Kansas City | — | $22.2M | 1 |
| KANSAS CITY UNITED METHODIST RETIREMENT HOME INC | Kansas City | P750 Human Services | $22.1M | 1 |
| SYNERGY SERVICES INC | Parkville | P460 Human Services | $22.1M | 8 |
| BIOMED VALLEY CORPORATION | Kansas City | H19 Medical Research | $22M | 3 |
| MISSOURI RURAL ELECTRIC COOPERATIVE | Palmyra | — | $22M | 17 |
| Economic Security Corporation of Southwest Area | Joplin | — | $21.9M | 1 |
| Alexian Brothers Medical Care Group NFP | St Louis | E21 Health Care | $21.9M | 6 |
| CHILD EVANGELISM FELLOWSHIP INC | Warrenton | O55Z Youth Development | $21.9M | 3 |
| PROVIDENCE PARK INC | St Louis | — | $21.8M | 9 |
| CROSSROADS CHARTER SCHOOLS | Kansas City | B29 Education | $21.7M | 6 |
| LEARFIELD COMMUNICATIONS GROUP BENEFIT TRUST INC | Jefferson City | Y20 Mutual & Membership Benefit | $21.7M | 0 |
| BLUCURRENT CREDIT UNION | Springfield | — | $21.7M | 8 |
| NATIONAL CORN GROWERS ASSOCIATION | Chesterfield | K20Z Food, Agriculture & Nutrition | $21.6M | 30 |
| BERGES FAMILY FOUNDATION | St Louis | W99 Public & Societal Benefit | $21.5M | 0 |
| HCFN TITLE HOLDING CORPORATION | Kansas City | K11 Food, Agriculture & Nutrition | $21.5M | 4 |
| ST PATRICK CENTER | St Louis | — | $21.5M | 8 |
| REGIONAL HEALTH CARE CLINIC INC | Sedalia | E30 Health Care | $21.5M | 14 |
| WESTMINSTER CHRISTIAN ACADEMY | Town Country | B250 Education | $21.5M | 6 |
| ACADEMIE LAFAYETTE | Kansas City | P40 Human Services | $21.4M | 2 |
| St Vincent Seton Specialty Hospital Inc | St Louis | G44Z Diseases, Disorders & Medical Disciplines | $21.4M | 10 |
| East Central Missouri Behavioral Health Services Inc | Mexico | F30 Mental Health & Crisis Intervention | $21.4M | 11 |
| Missouri Hospital Association | Jefferson City | — | $21.3M | 12 |
| BARTON COUNTY ELECTRIC COOP INC | Lamar | — | $21.2M | 14 |
| COMMUNITY BLOOD CENTER OF THE OZARKS | Springfield | E61 Health Care | $21.2M | 5 |
| JEFFERSON FRANKLIN COMMUNITY ACTION CORPORATION | Hillsboro | — | $21.2M | 1 |
| FAMILY GUIDANCE CENTER FOR BEHAVIORAL HEALTHCARE | Saint Joseph | F320 Mental Health & Crisis Intervention | $21.1M | 7 |
| THE SARAH COMMUNITY | St Louis | P750 Human Services | $21.1M | 7 |
| TELCOMM CREDIT UNION | Springfield | — | $20.9M | 7 |
| UNITED CREDIT UNION | Mexico | — | $20.8M | 9 |
| ST JOHN HEALTH SYSTEM FOUNDATION INC | St Louis | E110 Health Care | $20.7M | 8 |
| NORTH CENTRAL MISSOURI ELECTRIC COO | Milan | — | $20.6M | 12 |
