| NORTH CAROLINA SPORTS LEGACY FOUNDATION | Charlotte | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $24.1M | 0 |
| Alliance Preparatory Schools Inc | Charlotte | — | $24.1M | 0 |
| Lincoln Charter School Inc | Denver | B20 Education | $24M | 4 |
| YOUNG MEN'S CHRISTIAN ASSOCIATION OF | Hickory | P27Z Human Services | $23.8M | 3 |
| UNC MANAGEMENT COMPANY INC | Chapel Hill | — | $23.6M | 13 |
| Country Club of Landfall | Wilmington | P20 Human Services | $23.6M | 6 |
| ROWAN-CABARRUS YOUNG MEN'S CHRISTIAN ASSOCIATION | Salisbury | P27Z Human Services | $23.5M | 4 |
| UWHARRIE GREEN SCHOOL INC | Asheboro | B29 Education | $23.3M | 2 |
| Saint Marys School | Raleigh | B410 Education | $23.3M | 8 |
| THE CYPRESS OF RALEIGH CLUB INC | Raleigh | N99 Recreation & Sports | $23.3M | 5 |
| Charlotte Sports Foundation | Charlotte | N40 Recreation & Sports | $23.3M | 2 |
| PACE AT HOME INC | Newton | E50 Health Care | $23.2M | 4 |
| NORTH CAROLINA BIOTECHNOLOGY CENTER | Research Triangle Park | U500 Science & Technology | $23.1M | 9 |
| Greensboro College | Greensboro | B420 Education | $23.1M | 5 |
| ASHEVILLE SCHOOL INC | Asheville | B250 Education | $22.9M | 8 |
| FOUNDATION OF THE UNIVERSITY OF | Charlotte | — | $22.9M | 3 |
| THE TIMOTHY INITIATIVE INC | Raleigh | X21 Religion-Related | $22.7M | 7 |
| ROOF ABOVE INC | Charlotte | A23Z Arts, Culture & Humanities | $22.5M | 5 |
| INSTITUTE FOR MEDICAL RESEARCH INC | Durham | — | $22.4M | 4 |
| DHIC Inc | Raleigh | — | $22.1M | 9 |
| InReach | Charlotte | P73Z Human Services | $21.9M | 5 |
| STATE CHARTERED CREDIT UNIONS IN NORTH CAROLINA | Greensboro | — | $21.9M | 2 |
| CAROMONT HEALTH SERVICES | Gastonia | E20 Health Care | $21.8M | 5 |
| MCLEOD CENTERS FOR WELLBEING | Charlotte | F22Z Mental Health & Crisis Intervention | $21.7M | 4 |
| NEW HANOVER COUNTY FINANCING CORPORATION | Wilmington | W110 Public & Societal Benefit | $21.7M | 0 |
| Pine Lake Preparatory | Mooresville | B29 Education | $21.7M | 3 |
| HENDERSON COLLEGIATE INC | Henderson | B29 Education | $21.7M | 2 |
| ACTION PATHWAYS INC | Fayetteville | P20Z Human Services | $21.6M | 2 |
| PENDER EMS AND FIRE INC | Burgaw | M24 Public Safety, Disaster Preparedness & Relief | $21.4M | 11 |
| PENICK VILLAGE INC | Southern Pines | P750 Human Services | $21.4M | 8 |
| PIEDMONT ADVANTAGE CREDIT UNION | Winstonsalem | — | $21.4M | 8 |
| UNITED STATES BASEBALL FEDERATION INC | Cary | — | $21.4M | 10 |
| HOSPICE OF CLEVELAND COUNTY INC | Shelby | P74Z Human Services | $21.3M | 10 |
| Cape Fear Academy | Wilmington | B200 Education | $21.2M | 7 |
| Sugar Creek Charter School Inc | Charlotte | B292 Education | $21.2M | 3 |
| GREENSBORO COUNTRY CLUB INC | Greensboro | — | $21.1M | 10 |
| CAROLINA DAY SCHOOL INC | Asheville | B24Z Education | $21.1M | 6 |
| FAYETTEVILLE AREA HEALTH EDUCATION | Fayetteville | B54Z Education | $21M | 9 |
| MOUNTAIN COMMUNITY HEALTH PARTNERSHIP INC | Bakersville | E32Z Health Care | $21M | 10 |
| NEW HANOVER COMMUNITY HEALTH CENTER | Wilmington | — | $21M | 10 |
| GEORGE AND SARAH BUCHANAN FOUNDATION | Greensboro | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $20.9M | 0 |
| Saint Augustine's University | Raleigh | B420 Education | $20.9M | 3 |
| NORTH RIDGE COUNTRY CLUB INC | Raleigh | — | $20.8M | 7 |
| PIEDMONT TRIAD CHARITABLE FOUNDATION | Greensboro | — | $20.8M | 0 |
| HOSPICE OF IREDELL COUNTY INC | Statesville | P74Z Human Services | $20.7M | 9 |
| NORTH CAROLINA RATE BUREAU | Raleigh | — | $20.7M | 8 |
| HopeWay Foundation | Charlotte | E12 Health Care | $20.6M | 7 |
| UNITED WAY OF GREATER CHARLOTTE INC | Charlotte | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $20.6M | 5 |
| LIFESPAN INC | Charlotte | — | $20.5M | 4 |
| TELCO COMMUNITY CREDIT UNION | Candler | — | $20.5M | 1 |
