| Sid W Richardson Foundation | Fort Worth | Z99 Unknown | $44.6M | 4 |
| AMPP GLOBAL CENTER INC | Houston | — | $44.6M | 6 |
| Howard Payne University | Brownwood | B430 Education | $44.3M | 5 |
| THE HENDERSON-WESSENDORFF FOUNDATION | Richmond | — | $44.3M | 8 |
| ILA WEST GULF MARITIME ASSOCIATION | Houston | — | $44.2M | 0 |
| UNITED STATES BOWLING CONGRESS INC | Arlington | N70 Recreation & Sports | $44.2M | 8 |
| MOTIVATION EDUCATION AND TRAINING | New Caney | J220 Employment | $44M | 5 |
| M D ANDERSON SERVICES CORPORATION | Houston | E110 Health Care | $44M | 16 |
| FAITH FAMILY KIDS INC | DeSoto | B20 Education | $44M | 15 |
| OPERATION HOMEFRONT INC | San Antonio | S20 Community Improvement & Capacity Building | $43.9M | 5 |
| DALLAS JEWISH COMMUNITY FOUNDATION | Dallas | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $43.8M | 4 |
| Gainesville Community Hospital Inc | Gainesville | E20 Health Care | $43.7M | 13 |
| PERMIAN BASIN AREA FOUNDATION | Midland | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $43.7M | 5 |
| JAMES C & NORMA I SMITH FOUNDATION | Dallas | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $43.5M | 2 |
| A CHARTER SCHOOLS INC | Dallas | B200 Education | $43.4M | 7 |
| People's Community Clinic Inc | Austin | E32Z Health Care | $43.2M | 10 |
| TEXAS PIPE TRADES HEALTH BENEFIT PLAN | Richardson | — | $43M | 0 |
| Buffalo Bayou Partnership Inc | Houston | C110 Environment | $42.6M | 7 |
| CAMERON WORKS INC | Brownsville | J200 Employment | $42.4M | 0 |
| Texas A&M University - Kingsville | Kingsville | B43 Education | $42.3M | 0 |
| UNION SQUARE CREDIT UNION | Wichita Falls | — | $42M | 19 |
| The Gulf Coast Center | League City | G99 Diseases, Disorders & Medical Disciplines | $41.8M | 15 |
| HAMILTON COUNTY ELECTRIC COOPERATIVE | Hamilton | — | $41.7M | 14 |
| MHS Physicians of Texas | Houston | E32Z Health Care | $41.7M | 15 |
| DALLAS SYMPHONY ASSOCIATION | Dallas | A690 Arts, Culture & Humanities | $41.6M | 14 |
| SAN ANGELO AREA FOUNDATION | San Angelo | T99 Philanthropy, Voluntarism & Grantmaking Foundations | $41.5M | 3 |
| AUSTIN ACHIEVE PUBLIC SCHOOLS INC | Austin | B99 Education | $41.5M | 8 |
| ASSOCIATED CREDIT UNION OF TEXAS | League City | — | $41.5M | 5 |
| MEDICAL AND LIFE BENEFITS PLAN TRUST FOR | Plano | Y43 Mutual & Membership Benefit | $41.5M | 1 |
| HEAD START OF GREATER DALLAS INC | Dallas | B22Z Education | $41.4M | 4 |
| MOBILE LOAVES & FISHES INC | Austin | X22 Religion-Related | $41.4M | 9 |
| YOUNG MEN'S CHRISTIAN ASSOCIATION OF CENTRAL TEXAS | Round Rock | — | $41.4M | 15 |
| WESTMINSTER MANOR | Austin | E91Z Health Care | $41.3M | 6 |
| Foundation Communities Inc | Austin | L20Z Housing & Shelter | $41.3M | 10 |
| ISAS GROUP BENEFITS TRUST | Dallas | — | $41.2M | 25 |
| Houston Area Safety Council Inc | Pasadena | B600 Education | $40.9M | 13 |
| ALLIANCE CREDIT UNION | Lubbock | — | $40.8M | 12 |
| TEXAS ORGAN SHARING ALLIANCE | San Antonio | — | $40.8M | 10 |
| MEADOWS MENTAL HEALTH POLICY INSTITUTE | Dallas | F05 Mental Health & Crisis Intervention | $40.8M | 17 |
| LONE STAR LEGAL AID | Houston | I800 Crime & Legal-Related | $40.7M | 9 |
| BAPTIST COMMUNITY SERVICES | Amarillo | P750 Human Services | $40.7M | 25 |
| EDUCATION SERVICE CENTER REGION 12 | Waco | B90Z Education | $40.2M | 33 |
| TRAVIS ASSOCIATION FOR THE BLIND | Austin | G41 Diseases, Disorders & Medical Disciplines | $40.1M | 9 |
| Gateway Community Health Center Inc | Laredo | E32Z Health Care | $40M | 14 |
| FOOD BANK OF THE RIO GRANDE VALLEY INC | McAllen | — | $39.8M | 2 |
| CHILDCAREGROUP | Dallas | — | $39.7M | 6 |
| TRINITY CHRISTIAN ACADEMY | Addison | B24Z Education | $39.5M | 9 |
| LIGHTHOUSE ELECTRIC COOPERATIVE INC | Floydada | — | $39.4M | 17 |
| The West Texas Rehabilitation Center | Abilene | E240 Health Care | $39.3M | 13 |
| The Hughen Center Inc | Port Arthur | — | $39.3M | 7 |
