| The Gulf Coast Center | League City | G99 Diseases, Disorders & Medical Disciplines | $41.8M | 15 |
| CHAMPION EMS | Longview | E62 Health Care | $41.7M | 8 |
| HAMILTON COUNTY ELECTRIC COOPERATIVE ASSOCIATION | Hamilton | — | $41.7M | 14 |
| Main Street Community Initiatives | Bellaire | E99 Health Care | $41.7M | 19 |
| DALLAS SYMPHONY ASSOCIATION | Dallas | A690 Arts, Culture & Humanities | $41.6M | 14 |
| SAN ANGELO AREA FOUNDATION | San Angelo | T99 Philanthropy, Voluntarism & Grantmaking Foundations | $41.5M | 3 |
| AUSTIN ACHIEVE PUBLIC SCHOOLS INC | Austin | B99 Education | $41.5M | 8 |
| HEAD START OF GREATER DALLAS INC | Dallas | B22Z Education | $41.4M | 4 |
| MOBILE LOAVES & FISHES INC | Austin | X22 Religion-Related | $41.4M | 9 |
| YOUNG MEN'S CHRISTIAN ASSOCIATION OF CENTRAL TEXAS | Round Rock | — | $41.4M | 15 |
| Valor Texas Education Foundation | Austin | B12 Education | $41.2M | 6 |
| TEXAS WOMANS UNIVERSITY FOUNDATION INC | Denton | B110 Education | $41M | 0 |
| NORTH TEXAS PUBLIC BROADCASTING INC | Dallas | A300 Arts, Culture & Humanities | $41M | 10 |
| Houston Area Safety Council Inc | Pasadena | B600 Education | $40.9M | 13 |
| MY COMMUNITY CREDIT UNION | Midland | — | $40.8M | 10 |
| TEXAS ORGAN SHARING ALLIANCE | San Antonio | — | $40.8M | 10 |
| MEADOWS MENTAL HEALTH POLICY INSTITUTE FOR TEXAS | Dallas | F05 Mental Health & Crisis Intervention | $40.8M | 17 |
| LONE STAR LEGAL AID | Houston | I800 Crime & Legal-Related | $40.7M | 9 |
| BAPTIST COMMUNITY SERVICES | Amarillo | P750 Human Services | $40.7M | 25 |
| MORNINGSIDE MINISTRIES | San Antonio | P750 Human Services | $40.7M | 8 |
| BUCKNER SENIOR LIVING INC | Dallas | L22 Housing & Shelter | $40.6M | 14 |
| American College of Emergency Physicians | Dallas | — | $40.4M | 31 |
| THE TRINITY FAMILY FOUNDATION | Dallas | T21 Philanthropy, Voluntarism & Grantmaking Foundations | $40.3M | 0 |
| EDUCATION SERVICE CENTER REGION 12 | Waco | B90Z Education | $40.2M | 33 |
| TRAVIS ASSOCIATION FOR THE BLIND | Austin | G41 Diseases, Disorders & Medical Disciplines | $40.1M | 9 |
| Education Credit Union | Amarillo | — | $39.8M | 12 |
| CHILDCAREGROUP | Dallas | — | $39.7M | 6 |
| THE ELLIOTT FAMILY FOUNDATION | Nacogdoches | T90 Philanthropy, Voluntarism & Grantmaking Foundations | $39.5M | 0 |
| TRINITY CHRISTIAN ACADEMY | Addison | B24Z Education | $39.5M | 9 |
| LIGHTHOUSE ELECTRIC COOPERATIVE INC | Floydada | — | $39.4M | 17 |
| The West Texas Rehabilitation Center | Abilene | E240 Health Care | $39.3M | 13 |
| The Hughen Center Inc | Port Arthur | — | $39.3M | 7 |
| FAYETTE ELECTRIC COOPERATIVE INC | La Grange | — | $39.2M | 13 |
| WELBORN-PAYNE ENDOWMENT TRUST C/O PROSPERITY BANK TRUSTEE | Lubbock | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $39.2M | 1 |
| LIFENET INC | Texarkana | — | $39.1M | 5 |
| TEXAS CASA INC | Austin | R200 Civil Rights, Social Action & Advocacy | $39.1M | 7 |
| COLUMBUS COMMUNITY HOSPITAL | Columbus | E220 Health Care | $39.1M | 8 |
| Scott & White Hospital-Taylor | Dallas | E220 Health Care | $39.1M | 19 |
| SPECIAL HEALTH RESOURCES FOR TEXAS INC | Longview | — | $39M | 7 |
| United Medical Centers | Eagle Pass | E320 Health Care | $38.9M | 9 |
| Houston Symphony Society | Houston | A690 Arts, Culture & Humanities | $38.8M | 10 |
| PERMIAN BASIN COMMUNITY CENTERS FOR MHMR | Midland | F320 Mental Health & Crisis Intervention | $38.8M | 8 |
| Heavenly Fathers Foundation | Cisco | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $38.7M | 0 |
| YOUNG MEN'S CHRISTIAN ASSOCIATION OF GREATER SAN ANTONIO | San Antonio | — | $38.7M | 10 |
| BIG COUNTRY ELECTRIC COOPERATIVE INC | Roby | C35 Environment | $38.6M | 18 |
| Montgomery County Food Bank | Conroe | — | $38.5M | 2 |
| W W CARUTH JR FOUNDATION | Dallas | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $38.4M | 1 |
| SOUTHWEST TEXAS REGIONAL ADVISORY COUNCIL | San Antonio | E60 Health Care | $38.3M | 5 |
| One For Israel | Grapevine | X20 Religion-Related | $38.2M | 6 |
| CENTRAL TEXAS WORKFORCE DEVELOPMENT BOARD | Belton | J20 Employment | $38.2M | 0 |
