| COLLEGE OF ST SCHOLASTICA INC | Duluth | B420 Education | $112.4M | 8 |
| TRUSTEES OF THE HAMLINE UNIVERSITY OF MINNESOTA | St Paul | B430 Education | $111.8M | 12 |
| Winona Health Services | Winona | E220 Health Care | $110.5M | 15 |
| MINNESOTA PUBLIC RADIO AMERICAN PUBLIC MEDIA | St Paul | A340 Arts, Culture & Humanities | $108.7M | 18 |
| MINNESOTA VALLEY ELECTRIC COOPERATIVE | Jordan | — | $108.6M | 22 |
| ST MARYS UNIVERSITY OF MINNESOTA | Winona | B430 Education | $107.9M | 11 |
| CATHOLIC COMMUNITY FOUNDATION OF MINNESOTA | St Paul | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $107.3M | 8 |
| College of Saint Benedict | Saint Joseph | B420 Education | $105.7M | 11 |
| AITKIN COMMUNITY HOSPITAL DBA RIVERWOOD HEALTHCARE CENTER | Aitkin | E220 Health Care | $104.6M | 10 |
| THE RICHARD M SCHULZE FAMILY FOUNDATION | Minneapolis | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $102.8M | 1 |
| ASTERA HEALTH | Wadena | — | $102.4M | 18 |
| HUTCHINSON HEALTH | Hutchinson | E220 Health Care | $102.3M | 15 |
| NEXUS FAMILY HEALING | Plymouth | I400 Crime & Legal-Related | $99.1M | 17 |
| MINNESOTA HISTORICAL SOCIETY | Saint Paul | A800 Arts, Culture & Humanities | $96.8M | 9 |
| PRESBYTERIAN HOMES HOUSING & ASSISTED LIVING INC | Roseville | P750 Human Services | $95.6M | 9 |
| WESTFIELDS HOSPITAL INC | Minneapolis | E22 Health Care | $95.3M | 17 |
| READING AND MATH INC | Minneapolis | B92 Education | $94.7M | 7 |
| HIWAY CREDIT UNION | Saint Paul | — | $94.6M | 12 |
| 3M EMPLOYEES WELFARE BENEFITS ASSOCIATION TRUST I | St Paul | Y43 Mutual & Membership Benefit | $94.4M | 1 |
| GLENCOE REGIONAL HEALTH SERVICES | Glencoe | E21 Health Care | $94M | 10 |
| MISCELLANEOUS DRIVERS AND HELPERS LOCAL 638 HEALTH WELFARE EYE & DENTAL | Bloomington | — | $90.1M | 6 |
| OTTO BREMER TRUST | St Paul | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $89.4M | 4 |
| CROW WING COOPERATIVE POWER & LIGHT CO | Brainerd | — | $89.3M | 18 |
| MARGARET A CARGILL FOUNDATION | Eden Prairie | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $87.8M | 12 |
| VOLUNTEERS OF AMERICA NATIONAL SERVICES | Eden Prairie | — | $85.8M | 10 |
| AMERICAN BAPTIST HOMES OF THE MIDWEST | Eden Prairie | P750 Human Services | $84.4M | 8 |
| PARTNERS IN COMMUNITY SUPPORTS INC | St Paul | P820 Human Services | $83.9M | 8 |
| MIENERGY COOPERATIVE | Rushford | — | $82.5M | 18 |
| BENEDICTINE HEALTH SYSTEM | Minneapolis | E210 Health Care | $82.5M | 26 |
| UPPER MIDWEST ORGAN PROCUREMENT ORGANIZATION INC | Minneapolis | E65 Health Care | $82.5M | 17 |
| AMERY REGIONAL MEDICAL CENTER INC | Minneapolis | N22 Recreation & Sports | $81.9M | 23 |
| IBEW 292 HEALTH CARE PLAN | Brooklyn Park | — | $80.2M | 8 |
| UNITY FAMILY HEALTHCARE | Little Falls | — | $78.4M | 11 |
| PAUL BUNYAN RURAL TELEPHONE COOPERATIVE | Bemidji | — | $77.6M | 14 |
| Minnesota Teen Challenge Inc | Minneapolis | F200 Mental Health & Crisis Intervention | $76.6M | 8 |
| MINNESOTA TRANSITIONS CHARTER SCHOOL | Minneapolis | B99B Education | $76.5M | 16 |
| LifeCare Medical Center | Roseau | E220 Health Care | $75M | 21 |
| TEAMSTERS JOINT COUNCIL 32-EMPLOYERS HEALTH AND WELFARE FUND | Bloomington | — | $73M | 0 |
| COMMUNITY MEMORIAL HOSPITAL ASSOCIATION | Cloquet | E220 Health Care | $73M | 10 |
| SERVEMINNESOTA | Minneapolis | S20 Community Improvement & Capacity Building | $72.9M | 6 |
| Eventide | Moorhead | L220 Housing & Shelter | $71.8M | 8 |
| STEARNS COOPERATIVE ELECTRIC ASSOCIATION | Melrose | — | $71.5M | 16 |
| SHEET METAL #10 BENEFIT FUND | Maplewood | — | $71.3M | 7 |
| Fred C and Katherine B Andersen Foundation | Bayport | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $71.2M | 2 |
| FRASER CHILD AND FAMILY CENTER | Richfield | E99M Health Care | $71.1M | 7 |
| Goodwill Industries Inc | St Paul | — | $70.7M | 11 |
| FEED MY STARVING CHILDREN INC | Coon Rapids | Q330 International, Foreign Affairs & National Security | $70.5M | 10 |
| Knute Nelson | Alexandria | P20 Human Services | $69.6M | 5 |
| AUGUSTANA CARE | Edina | Z99Z Unknown | $69.5M | 11 |
| NORTHSTAR EDUCATION FINANCE INC | Eden Prairie | B822 Education | $68.1M | 9 |
