| THE SHAW UNIVERSITY | Raleigh | B420 Education | $36.2M | 11 |
| NEW CHARLOTTE CORPORATION | Charlotte | W190 Public & Societal Benefit | $35.9M | 2 |
| NC HISTORY CENTER ON THE CIVIL WAR | Fayetteville | A54 Arts, Culture & Humanities | $35.8M | 1 |
| CORNELIA NIXON DAVIS INC | Wilmington | E91Z Health Care | $35.5M | 5 |
| KIPP ENC PUBLIC SCHOOLS | Gaston | B29 Education | $35.3M | 1 |
| THE CHAPEL HILL RESIDENTIAL RETIREMENT | Chapel Hill | L220 Housing & Shelter | $35.1M | 7 |
| AHSNF INC | Charlotte | E24 Health Care | $35M | 8 |
| NC STATE STUDENT AID ASSOCIATION INC | Raleigh | B820 Education | $34.9M | 5 |
| ALEXANDER YOUTH NETWORK | Charlotte | P30 Human Services | $34.8M | 8 |
| PROJECT KITTY HAWK INC | Chapel Hill | B19 Education | $34.7M | 9 |
| ROANOKE CHOWAN COMMUNITY HEALTH CENTER | Ahoskie | E30 Health Care | $34.5M | 12 |
| CAROLINA VILLAGE INC | Hendersonville | L220 Housing & Shelter | $34.3M | 7 |
| The North Carolina A&T Real Estate Foundation Inc | Greensboro | B820 Education | $34.2M | 3 |
| INTER-FAITH FOOD SHUTTLE | Raleigh | P20Z Human Services | $34.2M | 1 |
| Children's Home Society of North Carolina Inc | Greensboro | P300 Human Services | $34.2M | 11 |
| ST JOSEPH OF THE PINES INC | Southern Pines | — | $34M | 9 |
| Greensboro Independent School Corp | Greensboro | B24Z Education | $33.9M | 6 |
| ALBEMARLE ELECTRIC MEMBERSHIP CORP | Hertford | — | $33.5M | 15 |
| MATERIAL HANDLING INDUSTRY | Charlotte | — | $32.9M | 13 |
| DON'T EVER GIVE UP INC | Cary | G11 Diseases, Disorders & Medical Disciplines | $32.8M | 9 |
| MECKLENBURG PARTNERSHIP FOR CHILDREN | Charlotte | B012 Education | $32.5M | 5 |
| Classical Charter Schools of America | Leland | B20 Education | $32.5M | 0 |
| CAHEC Management Inc | Raleigh | L11 Housing & Shelter | $32.4M | 13 |
| Montage Living | Charlotte | L20 Housing & Shelter | $32.3M | 0 |
| NORTH CAROLINA BANKERS ASSOCIATION | Raleigh | Y43 Mutual & Membership Benefit | $32.3M | 1 |
| EDGECOMBE-MARTIN COUNTY EMC | Tarboro | — | $32.2M | 14 |
| TRI-COUNTY COMMUNITY HEALTH COUNCIL INC | Four Oaks | — | $32.1M | 7 |
| APPALACHIAN STATE UNIVERSITY | Boone | — | $32.1M | 9 |
| CANNON SCHOOL INC | Concord | B24Z Education | $31.8M | 8 |
| WESTERN NORTH CAROLINA COMMUNITY HEALTH SERVICES INC | Asheville | — | $31.8M | 14 |
| ADVANCE COMMUNITY HEALTH INC | Raleigh | E32Z Health Care | $31.6M | 11 |
| PORT HEALTH SERVICES | Greenville | P30 Human Services | $31.6M | 13 |
| SERC RELIABILITY CORPORATION | Charlotte | C35 Environment | $31.5M | 13 |
| HALIFAX ELECTRIC MEMBERSHIP | Enfield | — | $31.5M | 15 |
| DUKE CORPORATE EDUCATION | Durham | B90 Education | $31.5M | 13 |
| The Forest At Duke Inc | Durham | P750 Human Services | $31.3M | 5 |
| LUDDY CHARITABLE FOUNDATION | Raleigh | T90 Philanthropy, Voluntarism & Grantmaking Foundations | $31.3M | 0 |
| Strickland Family Foundation | Winston Salem | T22Z Philanthropy, Voluntarism & Grantmaking Foundations | $31.2M | 1 |
| EDDIE AND JO ALLISON SMITH | Greenville | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $31M | 0 |
| ANONYMOUS TRUST | Raleigh | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $30.6M | 3 |
| APPALACHIAN MOUNTAIN COMMUNITY HEALTH CENTERS | Asheville | E21 Health Care | $30.6M | 11 |
| OPPORTUNITIES INDUSTRIALIZATION CENTER INC | Rocky Mount | — | $30.5M | 14 |
| Appalachian Regional Medical Associates Inc | Boone | E30 Health Care | $30.4M | 5 |
| TRIANGLE COMMUNITY FOUNDATION INC | Durham | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $30.2M | 5 |
| GOODWILL INDUSTRIES OF CENTRAL NORTH CAR | Greensboro | J32Z Employment | $29.8M | 9 |
| YOUNG MEN'S CHRISTIAN ASSOCIATION | Asheville | — | $29.7M | 8 |
| SENIOR TOTAL LIFE CARE | Gastonia | E40 Health Care | $29.7M | 6 |
| CHOWAN UNIVERSITY | Murfreesboro | B30 Education | $29.6M | 11 |
| DUMAC INC | Durham | B19 Education | $29.4M | 5 |
| CROSSNORE COMMUNITIES FOR CHILDREN | Crossnore | F330 Mental Health & Crisis Intervention | $29.4M | 10 |
