| Parker University | Dallas | B500 Education | $87.4M | 8 |
| CAREFLITE | Irving | E62 Health Care | $87M | 11 |
| CENTRAL TEXAS ELECTRIC COOPERATIVE INC | Fredericksburg | — | $86.9M | 20 |
| CHILDREN'S ADVOCACY CENTERS OF TEXAS INC | Austin | I72 Crime & Legal-Related | $85.9M | 12 |
| RAINWATER CHARITABLE FOUNDATION | Fort Worth | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $85.2M | 7 |
| TEXAS STATE AFFORDABLE HOUSING CORPORATION | Austin | L20 Housing & Shelter | $85.2M | 11 |
| SOUTH TEXAS BLOOD & TISSUE CENTER | San Antonio | E610 Health Care | $84.9M | 7 |
| Baylor Research Institute | Dallas | H900 Medical Research | $84.9M | 13 |
| BIENVIVIR SENIOR HEALTH SERVICES | El Paso | E3XZ Health Care | $84.8M | 7 |
| SCHARBAUER FOUNDATION INC | Midland | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $84.6M | 4 |
| Texas Health Harris Methodist Hospital Cleburne | Cleburne | E220 Health Care | $84.6M | 16 |
| KINDER FOUNDATION | Houston | T20Z Philanthropy, Voluntarism & Grantmaking Foundations | $84.5M | 7 |
| Hardin-Simmons University | Abilene | B430 Education | $84.3M | 13 |
| UTMB HEALTHCARE SYSTEMS INC | Galveston | E20 Health Care | $83M | 14 |
| Houston Zoo Inc | Houston | D115 Animal-Related | $82.5M | 9 |
| EAST TEXAS PROFESSIONAL CREDIT UNION | Longview | — | $82.5M | 11 |
| Houston Museum of Natural Science | Houston | A560 Arts, Culture & Humanities | $82.1M | 13 |
| The Kinkaid School Inc | Houston | B200 Education | $80M | 18 |
| Bowie-Cass Electric Cooperative Inc | Douglassville | — | $80M | 15 |
| LYNTEGAR ELECTRIC COOPERATIVE INC | Tahoka | — | $79.7M | 15 |
| United Way of Greater Houston | Houston | T70 Philanthropy, Voluntarism & Grantmaking Foundations | $79.6M | 11 |
| NEIGHBORHOOD CREDIT UNION | Dallas | — | $79.5M | 16 |
| BARBARA KAUFFMAN WELDER PRIVATE FOUNDATION | Victoria | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $79.5M | 3 |
| LIFESCHOOL OF DALLAS | Red Oak | B99Z Education | $79.4M | 11 |
| Allied Pilots Association % Hank Cofield | Fort Worth | — | $78.8M | 60 |
| ED RACHAL FOUNDATION | Corpus Christi | X99J Religion-Related | $78.6M | 7 |
| TRELLIS FOUNDATION | Round Rock | P20 Human Services | $78.3M | 3 |
| NATIONAL BOY SCOUTS OF AMERICA FOUNDATION | Irving | O41 Youth Development | $77.9M | 8 |
| STATE FAIR OF TEXAS | Dallas | N520 Recreation & Sports | $77.7M | 16 |
| Cook Children's Home Health | Fort Worth | E92 Health Care | $77.5M | 16 |
| TRUST FOR RETIREE MEDICAL DENTAL AND LIFE INSURANCE PLAN OF ARMY AND AIRFOR CE EXCHANGE SERVICE | Dallas | — | $77M | 0 |
| VICTORIA ELECTRIC COOPERATIVE INC | Victoria | — | $75.9M | 15 |
| The Moody Endowment | Houston | B280 Education | $75.7M | 3 |
| Texas Health Harris Methodist Hospital Stephenville | Stephenville | E220 Health Care | $75.4M | 14 |
| TEXAS BIOMEDICAL RESEARCH INSTITUTE | San Antonio | H40 Medical Research | $75.2M | 14 |
| DEAF SMITH ELECTRIC COOPERATIVE INC | Hereford | — | $75.2M | 14 |
| GREENHILL SCHOOL | Addison | B200 Education | $75.1M | 7 |
| FIRSTMARK CREDIT UNION | San Antonio | — | $75.1M | 11 |
| YMCA OF METROPOLITAN DALLAS INC | Coppell | P270 Human Services | $74.8M | 15 |
| Hendrick Provider Network | Abilene | E32Z Health Care | $74.6M | 26 |
| Baylor Health Care System Foundation | Dallas | E12 Health Care | $74.6M | 10 |
| The University of TexasTexas A&M Investment Management Company | Austin | B43B Education | $74.6M | 24 |
| Texas Health Hospital Mansfield | Mansfield | E22 Health Care | $74.4M | 9 |
| REWILD | Austin | D30 Animal-Related | $74.2M | 8 |
| TEXAS A&M RESEARCH FOUNDATION | College Station | B110 Education | $73.7M | 0 |
| OUR LADY OF THE LAKE UNIVERSITY OF SAN ANTONIO | San Antonio | B43 Education | $73.6M | 8 |
| FMH FOUNDATION | Midland | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $73.5M | 1 |
| Dallas Theological Seminary | Dallas | B500 Education | $73.4M | 15 |
| KATHERINE C CARMODY CHARITABLE TUW | Dallas | — | $73.2M | 1 |
| WORKFORCE SOLUTIONS BORDERPLEX INC | El Paso | J20 Employment | $72.3M | 2 |
