| GALLAUDET UNIVERSITY | Washington | B430 Education | $235M | 21 |
| International Baccalaureate Organization | Washington | B90 Education | $224.7M | 19 |
| THE CORPORATION FOR TRAVEL | Washington | S41 Community Improvement & Capacity Building | $224.5M | 10 |
| INTERNATIONAL BROTHERHOOD OF TEAMSTERS | Washington | — | $223.8M | 54 |
| KIPP DC PUBLIC CHARTER SCHOOLS | Washington | B20 Education | $212.6M | 12 |
| WINDWARD FUND | Washington | C30 Environment | $212.4M | 6 |
| AMERICAN FEDERATION OF TEACHERS AFL-CIO | Washington | — | $211M | 8 |
| NATIONAL GEOGRAPHIC SOCIETY | Washington | B030 Education | $209.3M | 31 |
| Humane World for Animals Inc | Washington | D200 Animal-Related | $207.9M | 18 |
| NEIGHBORHOOD REINVESTMENT CORPORATION | Washington | — | $205.7M | 13 |
| AMERICAN NEAR EAST REFUGEE AID | Washington | Q300 International, Foreign Affairs & National Security | $204.3M | 9 |
| PATIENT ACCESS NETWORK FOUNDATION | Washington | — | $203M | 22 |
| AARP FOUNDATION | Washington | P81 Human Services | $202.9M | 13 |
| Chamber of Commerce of the USA | Washington | — | $196.5M | 13 |
| INDIAN PAINTBRUSH FOUNDATION | Washington | B82I Education | $194.7M | 0 |
| INTERNATIONAL BROTHERHOOD OF | Washington | — | $190.8M | 32 |
| AMERICAN FEDERATION OF STATE COUNTY | Washington | — | $190.3M | 55 |
| HEALTH SERVICES FOR CHILDREN WITH | Washington | P82 Human Services | $190.2M | 10 |
| PROJECT HOPE - THE PEOPLE-TO-PEOPLE | Washington | Q300 International, Foreign Affairs & National Security | $186.5M | 15 |
| PACT INC | Washington | Q300 International, Foreign Affairs & National Security | $181.8M | 11 |
| NATIONAL DEMOCRATIC INSTITUTE FOR | Washington | Q300 International, Foreign Affairs & National Security | $181.5M | 15 |
| SIXTEEN THIRTY FUND | Washington | C60 Environment | $181.4M | 6 |
| AMERICAN CHEMISTRY COUNCIL | Washington | — | $180.8M | 15 |
| CONSORTIUM FOR ELECTIONS & | Washington | W24 Public & Societal Benefit | $177.7M | 0 |
| NATIONAL REHABILITATION HOSPITAL | Washington | E240 Health Care | $176M | 15 |
| AMERICAN BANKERS ASSOCIATION | Washington | — | $175.4M | 13 |
| The United States Holocaust Memorial Museum | Washington | A540 Arts, Culture & Humanities | $172.5M | 13 |
| UNITY HEALTH CARE INC | Washington | E320 Health Care | $167.9M | 8 |
| NCRC COMMUNITY DEVELOPMENT FUND INC | Washington | S40 Community Improvement & Capacity Building | $164.7M | 1 |
| ELIZABETH GLASER PEDIATRIC AIDS FOUNDATION | Washington | H810 Medical Research | $162.3M | 19 |
| DKT INTERNATIONAL INC | Washington | Q300 International, Foreign Affairs & National Security | $162.2M | 8 |
| INTERNATIONAL BROTHERHOOD OF ELECTRICAL | Washington | — | $160.5M | 12 |
| FRIENDSHIP PUBLIC CHARTER SCHOOL INC | Washington | B200 Education | $160.2M | 11 |
| GREATER WASHINGTON URBAN LEAGUE INC | Washington | P22Z Human Services | $159.9M | 5 |
| HOPEWELL FUND | Washington | T12 Philanthropy, Voluntarism & Grantmaking Foundations | $157.7M | 6 |
| RFEFL INC | Washington | A34Z Arts, Culture & Humanities | $156.5M | 14 |
| AMERICAN ISRAEL PUBLIC AFFAIRS COMMITTEE | Washington | Q20 International, Foreign Affairs & National Security | $156.4M | 12 |
| CHILDREN'S NATIONAL MEDICAL CENTER | Washington | E24Z Health Care | $155.7M | 19 |
| SPECIAL OLYMPICS INC | Washington | N720 Recreation & Sports | $152.2M | 12 |
| THE COMMUNITY PARTNERSHIP FOR THE | Washington | — | $150.9M | 7 |
| United Mission For Relief & Development | Washington | M20 Public Safety, Disaster Preparedness & Relief | $149.9M | 3 |
| NATIONAL FOOTBALL LEAGUE PLAYERS | Washington | J40 Employment | $148.2M | 48 |
| INTERNATIONAL RESEARCH & EXCHANGES | Washington | Q200 International, Foreign Affairs & National Security | $146.5M | 13 |
| COMMUNICATIONS WORKERS OF AMERICA | Washington | — | $144.1M | 25 |
| BRIGHT FUTURE FUND | Washington | — | $143.1M | 0 |
| American Psychological Association | Washington | V030 Social Science | $138.3M | 37 |
| ACDIVOCA | Washington | Q310 International, Foreign Affairs & National Security | $133.4M | 14 |
| FOUNDATION FOR FOOD AND AGRICULTURE | Washington | K99 Food, Agriculture & Nutrition | $132.3M | 5 |
| NATIONAL IAM BENEFIT TRUST FUND | Washington | — | $131.4M | 7 |
| PAN AMERICAN DEVELOPMENT FOUNDATION | Washington | Q300 International, Foreign Affairs & National Security | $131.1M | 12 |
