| Food Research & Action Center | Washington | K300 Food, Agriculture & Nutrition | $20.8M | 9 |
| HILLWOOD ESTATE MUSEUM & GARDENS | Washington | A80 Arts, Culture & Humanities | $20.8M | 8 |
| COLORECTAL CANCER ALLIANCE | Washington | E60 Health Care | $20.7M | 13 |
| FOOD & WATER WATCH | Washington | K05 Food, Agriculture & Nutrition | $20.7M | 6 |
| PROGRESSIVE LIFE CENTER INC | Washington | — | $20.7M | 0 |
| CULTURAL VISTAS INC | Washington | Q230 International, Foreign Affairs & National Security | $20.7M | 8 |
| PLANNED PARENTHOOD OF METROPOLITAN WASHINGTON DC INC | Washington | E420 Health Care | $20.7M | 13 |
| LATIN AMERICAN YOUTH CENTER | Washington | O200 Youth Development | $20.6M | 6 |
| THE AMERICAN SOCIETY FOR REPRODUCTIVE MEDICINE | Washington | — | $20.6M | 13 |
| AMERICAN ASSOCIATION OF COLLEGES OF NURSING | Washington | B030 Education | $20.6M | 8 |
| RACE FORWARD | Washington | S50Z Community Improvement & Capacity Building | $20.5M | 10 |
| THE SHAKESPEARE THEATRE | Washington | A650 Arts, Culture & Humanities | $20.4M | 8 |
| BOYS AND GIRLS CLUBS OF GREATER WASHINGTON AND AFFILIATE | Washington | — | $20.4M | 9 |
| JACK & JILL OF AMERICA INC | Washington | — | $20.4M | 0 |
| UAW RETIREES HEALTH CARE TRUST | Washington | Y43 Mutual & Membership Benefit | $20.4M | 9 |
| AMERICAN FOREST FOUNDATION | Washington | C36Z Environment | $20.3M | 10 |
| THE CONSUMER BANKERS ASSOCIATION | Washington | — | $20.2M | 10 |
| ACHIEVEMENT PREPARATORY ACADEMY PUBLIC CHARTER SCHOOL | Washington | B29 Education | $20.2M | 5 |
| IMPETUS FUND | Washington | — | $20.2M | 1 |
| ALLIANCE FOR YOUTH ORGANIZING | Washington | R40 Civil Rights, Social Action & Advocacy | $20.2M | 6 |
| CHILDREN'S DEFENSE FUND | Washington | R200 Civil Rights, Social Action & Advocacy | $20.2M | 11 |
| LAND TRUST ALLIANCE INC | Washington | C033 Environment | $20.1M | 10 |
| DRUG INFORMATION ASSOCIATION INC | Washington | E030 Health Care | $20M | 14 |
| COUNCIL OF INDEPENDENT COLLEGES | Washington | — | $19.9M | 13 |
| CENTER FOR SCIENCE IN THE PUBLIC INTEREST | Washington | K050 Food, Agriculture & Nutrition | $19.8M | 8 |
| LOWELL SCHOOL | Washington | B240 Education | $19.8M | 8 |
| NUCLEAR THREAT INITIATIVE INC | Washington | Q41 International, Foreign Affairs & National Security | $19.6M | 18 |
| THE MAYA ANGELOU PUBLIC CHARTER SCHOOL | Washington | B25 Education | $19.6M | 10 |
| BLAIR HOUSE FOUNDATION | Washington | A82Z Arts, Culture & Humanities | $19.5M | 2 |
| FUTURE NOW ACTION | Washington | W05 Public & Societal Benefit | $19.5M | 6 |
| NAFSA ASSOCIATION OF INTERNATIONAL EDUCATORS | Washington | Q220 International, Foreign Affairs & National Security | $19.5M | 10 |
| ASSOCIATION OF PUBLIC AND LAND-GRANT UNIVERSITIES | Washington | B40C Education | $19.4M | 11 |
| THE COMPUTING RESEARCH ASSOCIATION INC | Washington | — | $19.3M | 10 |
| BUILDING BRIDGES ACROSS THE RIVER INC | Washington | S99 Community Improvement & Capacity Building | $19.3M | 4 |
| ORDER OF AHEPA AHEPA FOUNDATION INC | Washington | Y40 Mutual & Membership Benefit | $19.2M | 0 |
| CENTRONIA | Washington | P33B Human Services | $19.2M | 4 |
| PATHWAYS TO HOUSING DC | Washington | P85 Human Services | $19.2M | 8 |
| DIGITAL PIONEERS ACADEMY PUBLIC CHARTER SCHOOL | Washington | B29 Education | $19.2M | 6 |
| SIBLEY MEMORIAL HOSPITAL FOUNDATION | Washington | E112 Health Care | $19.2M | 5 |
| DC BILINGUAL PUBLIC CHARTER SCHOOL | Washington | B29 Education | $19.1M | 7 |
| JUBILEE HOUSING INC | Washington | — | $19.1M | 5 |
| BREAD FOR THE CITY INC | Washington | K30Z Food, Agriculture & Nutrition | $19M | 5 |
| UNITED STATES TELECOM ASSOCIATION | Washington | — | $19M | 11 |
| THE KAILASH SATYARTHI CHILDREN'S FOUNDATION OF AMERICA | Washington | Q70 International, Foreign Affairs & National Security | $18.9M | 1 |
| BRIGHT BEGINNINGS INC | Washington | — | $18.9M | 3 |
| NHT COMMUNITIES | Washington | L210 Housing & Shelter | $18.9M | 6 |
| COMPUTER AND COMMUNICATIONS INDUSTRY ASSOCIATION | Washington | — | $18.9M | 8 |
| FINSECA | Washington | S41 Community Improvement & Capacity Building | $18.9M | 9 |
| THE ASIAN AMERICAN FOUNDATION | Washington | R22 Civil Rights, Social Action & Advocacy | $18.9M | 6 |
| PRIORITIES USA FOUNDATION | Washington | R20 Civil Rights, Social Action & Advocacy | $18.9M | 0 |
