| ACCION INTERNATIONAL | Washington | Q320 International, Foreign Affairs & National Security | $23.8M | 15 |
| INSTITUTE OF SCRAP RECYCLING INDUSTRIES INC | Washington | — | $23.6M | 15 |
| CENTER FOR GLOBAL DEVELOPMENT | Washington | P20 Human Services | $23.6M | 10 |
| UAW RETIREES OF THE BUDD COMPANY HEALTH AND WELFARE TRUST | Washington | Y43 Mutual & Membership Benefit | $23.6M | 5 |
| INTERNATIONAL CRISIS GROUP | Washington | Q70Z International, Foreign Affairs & National Security | $23.5M | 9 |
| NATIONAL INSTITUTE OF BUILDING SCIENCES | Washington | M20Z Public Safety, Disaster Preparedness & Relief | $23.4M | 8 |
| FOOD & FRIENDS INC | Washington | G81Z Diseases, Disorders & Medical Disciplines | $23.4M | 9 |
| CERTIFIED AUTOMOTIVE PARTS ASSOCIATION | Washington | — | $23.3M | 1 |
| VITAL VOICES GLOBAL PARTNERSHIP INC | Washington | Q300 International, Foreign Affairs & National Security | $23.2M | 9 |
| NORTH AMERICAN SECURITIES ADMINISTRATORS ASSOCIATION INC | Washington | Z99Z Unknown | $23.1M | 7 |
| NATIONAL LOW INCOME HOUSING COALITION | Washington | L99Z Housing & Shelter | $23.1M | 6 |
| WAY TO RISE | Washington | B82 Education | $23M | 0 |
| NPR FOUNDATION | Washington | — | $23M | 5 |
| BLACK ECONOMIC ALLIANCE FOUNDATION | Washington | T11 Philanthropy, Voluntarism & Grantmaking Foundations | $22.9M | 2 |
| CONSUMER HEALTHCARE PRODUCTS ASSOCIATION | Washington | — | $22.9M | 13 |
| Hudson Institute Inc | Washington | — | $22.7M | 13 |
| THE FEDERALIST SOCIETY FOR LAW AND PUBLIC POLICY STUDIES | Washington | — | $22.5M | 11 |
| iMMAP Inc | Washington | Q30 International, Foreign Affairs & National Security | $22.4M | 4 |
| CENTER FOR AMERICAN PROGRESS ACTION FUND | Washington | W01 Public & Societal Benefit | $22.4M | 8 |
| THE PERSONAL CARE PRODUCTS COUNCIL INC | Washington | — | $22.4M | 14 |
| RETAIL INDUSTRY LEADERS ASSOCIATION INC | Washington | — | $22.4M | 11 |
| HOUSE MAJORITY FORWARD | Washington | S01 Community Improvement & Capacity Building | $22.3M | 7 |
| ASSOCIATION FOR ACCESSIBLE MEDICINES | Washington | S41 Community Improvement & Capacity Building | $22.2M | 9 |
| INFORMATION TECHNOLOGY INDUSTRY COUNCIL | Washington | — | $22.2M | 12 |
| Girl Scout Council of the Nation's Capital | Washington | O42Z Youth Development | $22.1M | 5 |
| EDUCATION FORWARD DC | Washington | B19 Education | $21.9M | 10 |
| AMERICAN ASTRONOMICAL SOCIETY | Washington | U31 Science & Technology | $21.9M | 9 |
| Physicians Committee for Responsible Med | Washington | E85Z Health Care | $21.9M | 6 |
| MEDIA MATTERS FOR AMERICA | Washington | B99 Education | $21.9M | 14 |
| EUGENE & AGNES E MEYER FOUNDATION | Washington | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $21.8M | 2 |
| ENVIRONMENTAL WORKING GROUP | Washington | C35 Environment | $21.8M | 9 |
| SCORE Association | Washington | — | $21.7M | 11 |
| ST COLETTA SPECIAL EDUCATION PUBLIC CHARTER SCHOOL | Washington | B11 Education | $21.7M | 5 |
| UAW RETIREES OF DANA CORPORATION HEALTH AND WELFARE TRUST | Washington | Y43 Mutual & Membership Benefit | $21.6M | 7 |
| WASHINGTON INSTITUTE FOR NEAR EAST POLICY | Washington | Q055 International, Foreign Affairs & National Security | $21.5M | 11 |
| THE GLOBAL DEVELOPMENT INCUBATOR INC | Washington | Q32 International, Foreign Affairs & National Security | $21.5M | 8 |
| MERIDIAN PUBLIC CHARTER SCHOOL | Washington | B20 Education | $21.5M | 6 |
| The National Restaurant Association Educational Foundation | Washington | K99E Food, Agriculture & Nutrition | $21.4M | 12 |
| BANCROFT GLOBAL DEVELOPMENT | Washington | Q41 International, Foreign Affairs & National Security | $21.3M | 0 |
| NORTH AMERICAN REGION OF THE AIRPORTS COUNCIL INTERNATIONAL | Washington | — | $21.3M | 9 |
| ATLANTIC 10 CONFERENCE | Washington | N400 Recreation & Sports | $21.3M | 5 |
| NATIONAL LEAGUE OF CITIES INSTITUTE INC | Washington | B11 Education | $21.3M | 7 |
| THE FUND FOR AMERICAN STUDIES | Washington | — | $21.2M | 9 |
| CENTRAL UNION MISSION | Washington | L410 Housing & Shelter | $21.1M | 3 |
| NATIONAL EDUCATION ASSOCIATION AND AFFILIATE RETIREE HEALTH PLAN | Washington | Y43 Mutual & Membership Benefit | $21.1M | 3 |
| THE WASHINGTON CENTER FOR INTERNSHIPS & ACADEMIC SEMINARS | Washington | — | $21.1M | 8 |
| AMERICAN IMMIGRATION LAWYERS ASSOCIATION | Washington | — | $20.9M | 7 |
| AMERICAN COUNCIL OF ENGINEERING COMPANIES | Washington | — | $20.9M | 14 |
| MILTON GOTTESMAN JEWISH DAY SCHOOL OF THE NATION'S CAPITAL | Washington | B20 Education | $20.9M | 7 |
| HEART RHYTHM SOCIETY INC | Washington | — | $20.8M | 14 |
