| WEST CENTRAL COMMUNITY ACTION | Harlan | P200 Human Services | $14.6M | 2 |
| CEDAR VALLEY HOSPICE INC | Waterloo | P74Z Human Services | $14.6M | 7 |
| BARTELS LUTHERAN HOME | Waverly | P750 Human Services | $14.5M | 4 |
| SUNRISE MANOR | Sioux City | E910 Health Care | $14.5M | 3 |
| CHARLES E LAKIN FOUNDATION INC | Council Bluffs | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $14.5M | 8 |
| COMMUNITY FOUNDATION OF NORTHEAST IOWA | Cedar Falls | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $14.5M | 4 |
| HARBOR GROUP INSURANCE TRUST | Sioux Center | — | $14.4M | 0 |
| ALL CARE HEALTH CENTER | Council Bluffs | E30 Health Care | $14.4M | 6 |
| Chariton Valley Electric Cooperative Inc | Albia | — | $14.3M | 17 |
| MID-SIOUX OPPORTUNITY INC | Remsen | P200 Human Services | $14.3M | 3 |
| NORTHEAST IOWA COMMUNITY ACTION CORP | Decorah | P200 Human Services | $14.3M | 2 |
| PRAIRIE RIDGE INTEGRATED BEHAVIORAL HEALTHCARE | Mason City | F21 Mental Health & Crisis Intervention | $14.2M | 7 |
| SOUTHWEST IOWA REC | Corning | M20 Public Safety, Disaster Preparedness & Relief | $14.2M | 13 |
| Lutheran Home for the Aged Association - West | Perry | L220 Housing & Shelter | $14.2M | 4 |
| UNITYPOINT HEALTH AT WORK | West Des Moines | E32 Health Care | $14.1M | 8 |
| CHILDSERVE HOMES INC | Johnston | — | $14M | 4 |
| IOWA STATE UNIVERSITY RESEARCH PARK | Ames | B110 Education | $14M | 3 |
| CHILDSERVE FOUNDATION INC | Johnston | P120 Human Services | $14M | 3 |
| MERCYCARE SERVICE CORPORATION | Cedar Rapids | — | $14M | 3 |
| STL CARE COMPANY | Cedar Rapids | E24 Health Care | $13.9M | 4 |
| UNITED COMMUNITY SERVICES INC | West Des Moines | F20 Mental Health & Crisis Intervention | $13.9M | 8 |
| LYON RURAL ELECTRIC COOPERATIVE | Rock Rapids | — | $13.9M | 15 |
| EASTERN STAR MASONIC HOME | Boone | P75Z Human Services | $13.8M | 1 |
| CHRISTIAN OPPORTUNITY CENTER | Pella | J30 Employment | $13.8M | 3 |
| ERIC AND KELLY SCHWARTZ CHARITABLE TRUST | Fairfield | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $13.7M | 2 |
| CENTRAL COMMUNITY HOSPITAL | Elkader | E22Z Health Care | $13.7M | 7 |
| IOWA STATE EDUCATION ASSOCIATION | Des Moines | — | $13.5M | 10 |
| COMMUNITY OPPORTUNITIES INC | Carroll | P200 Human Services | $13.5M | 3 |
| METHODIST MANOR RETIREMENT COMMUNITY | Storm Lake | E91Z Health Care | $13.4M | 1 |
| COMMUNITY HEALTH CENTER OF FORT DODGE INC | Fort Dodge | E70 Health Care | $13.4M | 4 |
| BETHANY LUTHERAN HOME | Council Bluffs | — | $13.3M | 0 |
| REGION XII COUNCIL OF GOVERNMENTS INC | Carroll | L210 Housing & Shelter | $13.2M | 1 |
| SCHWARTZ FAMILY FOUNDATION | Fairfield | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $13.2M | 0 |
| CHILDSERVE THERAPY INC | Johnston | — | $13.2M | 3 |
| COMMUNITY ACTION AGENCY OF SIOUXLAND | Sioux City | — | $12.8M | 2 |
| RESEARCH FOUNDATION FOR MENTAL HYGIENE INC EMPLOYER CONTRIBUTION VEBA TRUST | Clinton | Y43 Mutual & Membership Benefit | $12.8M | 2 |
| NISHNA PRODUCTIONS INC | Shenandoah | P73Z Human Services | $12.8M | 1 |
| WOODBURY COUNTY RURAL ELECTRIC COOPERATIVE | Moville | — | $12.8M | 14 |
| SOUTHERN IOWA ECONOMIC DEVELOPMENT ASSOCIATION | Ottumwa | — | $12.7M | 3 |
| LAURIDSEN FAMILY FOUNDATION | Ankeny | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $12.7M | 0 |
| CEDAR FALLS COMMUNITY CREDIT UNION | Cedar Falls | — | $12.6M | 1 |
| BLANK PARK ZOO FOUNDATION INC | Des Moines | D115 Animal-Related | $12.6M | 3 |
| PROGRESS INDUSTRIES | Newton | J30Z Employment | $12.5M | 2 |
| MAHARISHI FOUNDATION USA INC | Fairfield | — | $12.4M | 3 |
| SCOTTISH RITE PARK INC | Des Moines | — | $12.3M | 5 |
| OPERATION THRESHOLD INC | Waterloo | — | $12.2M | 2 |
| ALLEN COLLEGE | Waterloo | — | $12.2M | 5 |
| PLYMOUTH LIFE INC | Le Mars | P820 Human Services | $12.2M | 3 |
| MANATT'S INC GROUP HEALTH PLAN TRUST | Brooklyn | — | $12.2M | 0 |
| Quad City Helicopter Emergency Med Service | Bettendorf | M23 Public Safety, Disaster Preparedness & Relief | $12.1M | 5 |
