| CONSUMER CREDIT UNION | Greeneville | — | $27.3M | 6 |
| HABITAT FOR HUMANITY OF GREATER | Nashville | — | $27.2M | 8 |
| Pi Kappa Alpha Foundation | Memphis | B110 Education | $27.2M | 3 |
| SOUTHERN PAINTERS WELFARE FUND | Goodlettsville | — | $27.2M | 2 |
| UPPER EAST TENNESSEE HUMAN | Kingsport | S200 Community Improvement & Capacity Building | $26.9M | 2 |
| BAPTIST MEMORIAL HOSPITAL-CRITTENDEN | Memphis | E24 Health Care | $26.9M | 17 |
| RICHLAND PLACE INC | Murfreesboro | P750 Human Services | $26.9M | 13 |
| FAIRFIELD GLADE COMMUNITY CLUB | Fairfield Glade | N31 Recreation & Sports | $26.8M | 7 |
| CATHOLIC CHARITIES OF TENNESSEE INC | Nashville | — | $26.8M | 4 |
| Alliance Health Services Inc | Memphis | E92Z Health Care | $26.8M | 11 |
| HOOSIER CARE PROPERTIES INC | Nashville | — | $26.4M | 8 |
| HIGHLAND TELEPHONE COOPERATIVE INC | Sunbright | W50Z Public & Societal Benefit | $26.4M | 21 |
| FEED AMERICA FIRST OF TENNESSEE | Murfreesboro | K31 Food, Agriculture & Nutrition | $26.3M | 1 |
| PSALMS INC | Memphis | P750 Human Services | $26M | 9 |
| THE VILLAGE AT GERMANTOWN INC | Germantown | P75 Human Services | $25.8M | 8 |
| HCA HEALTHCARE FOUNDATION | Nashville | B82 Education | $25.8M | 0 |
| Memphis Zoological Society | Memphis | D500 Animal-Related | $25.8M | 6 |
| FORKED DEER ELECTRIC COOPERATIVE | Halls | — | $25.8M | 10 |
| THE HT HACKNEY CO VEBA | Knoxville | — | $25.6M | 1 |
| AHB FOUNDATION INC | Knoxville | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $25.4M | 0 |
| THE HUTCHISON SCHOOL | Memphis | B200 Education | $25.4M | 7 |
| WOODBINE COMMUNITY ORGANIZATION INC | Nashville | S200 Community Improvement & Capacity Building | $25.3M | 1 |
| KIPP MEMPHIS | Memphis | B29 Education | $25.2M | 7 |
| HARDIN COUNTY REGIONAL HEALTH CENTER INC | Savannah | — | $25.2M | 10 |
| CURREY INGRAM ACADEMY | Brentwood | B28Z Education | $25.2M | 7 |
| LIFE BRIDGES INC | Cleveland | P82Z Human Services | $25.2M | 1 |
| HIGHLANDER RESEARCH & EDUCATION CENTER | New Market | C60Z Environment | $25M | 4 |
| 9TH AVENUE FOUNDATION | Knoxville | — | $25M | 0 |
| NEA BAPTIST HEALTH SYSTEM INC | Memphis | E21 Health Care | $25M | 17 |
| GOVERNOR'S EARLY LITERACY FOUNDATION | Nashville | B92 Education | $24.9M | 2 |
| NEW DAY SCHOOLS INC | Memphis | B29 Education | $24.9M | 4 |
| UNITED NEIGHBORHOOD HEALTH SERVICES INC | Nashville | — | $24.6M | 11 |
| MADISON HAYWOOD DEVELOPMENTAL | Jackson | P820 Human Services | $24.5M | 2 |
| YOUNG MEN'S CHRISTIAN ASSOCIATION | Chattanooga | — | $24.4M | 6 |
| KENCO GROUP INC VOLUNTARY EMPLOYEE | Chattanooga | — | $24.3M | 0 |
| NASHVILLE RESCUE MISSION | Nashville | L41 Housing & Shelter | $24.1M | 5 |
| CAPSTONE EDUCATION GROUP INC | Memphis | B20 Education | $23.8M | 6 |
| Co-op Health and Welfare Plan & Trust | Lavergne | — | $23.7M | 0 |
| CHURCH HEALTH CENTER OF MEMPHIS INC | Memphis | E320 Health Care | $23.6M | 19 |
| Buffalo Valley Inc | Hohenwald | F22Z Mental Health & Crisis Intervention | $23.6M | 4 |
| HILLWOOD COUNTRY CLUB | Nashville | — | $23.6M | 7 |
| SEEDING SUCCESS | Memphis | O50 Youth Development | $23.6M | 5 |
| SOUTHEASTERN CARP & MW HEALTH PLAN | Goodlettsville | — | $23.4M | 0 |
| B'NAI B'RITH HOME AND HOSPITAL FOR AGED | Cordova | E910 Health Care | $23.4M | 8 |
| THE BLAKEFORD AT GREEN HILLS | Nashville | P750 Human Services | $23.2M | 8 |
| Lorraine Civil Rights Museum Foundation | Memphis | A540 Arts, Culture & Humanities | $23.1M | 8 |
| THE FRIST FOUNDATION | Nashville | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $23.1M | 3 |
| AVE MARIA HOME INC | Memphis | P750 Human Services | $23.1M | 5 |
| MID-CUMBERLAND COMMUNITY ACTION AGENCY | Gallatin | P20Z Human Services | $23M | 2 |
| Good Shepherd Medication Management | Memphis | Z99 Unknown | $23M | 1 |
