| BEN LOMAND RURAL TELEPHONE COOPERATIVE | McMinnville | — | $36.6M | 18 |
| MEMPHIS UNIVERSITY SCHOOL | Memphis | B250 Education | $36.4M | 8 |
| NASHVILLE ZOO INC | Nashville | D500 Animal-Related | $35.9M | 11 |
| DETTWILLER FOUNDATION | Nashville | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $35.9M | 3 |
| CHATTANOOGA CHRISTIAN COMMUNITY | Chattanooga | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $35.7M | 1 |
| BRENTWOOD ACADEMY | Brentwood | B24Z Education | $35.5M | 7 |
| UNITED FOOD & COMMERICAL WORKERS EMPL | Cordova | — | $35.5M | 0 |
| FREEDOM PREPARATORY ACADEMY INC | Memphis | B24 Education | $35.4M | 7 |
| MARTHA O'BRYAN CENTER INC | Nashville | X20 Religion-Related | $34.7M | 5 |
| BAPTIST MEMORIAL HOSPITAL- | Huntingdon | E220 Health Care | $34.7M | 11 |
| OPERATION SHARING TENNESSEE | Oneida | P122 Human Services | $33.9M | 0 |
| SOUTHEASTERN IRON WORKERS WELFARE PLAN | Goodlettsville | — | $33.8M | 0 |
| WEBB SCHOOL OF KNOXVILLE | Knoxville | B200 Education | $33.7M | 12 |
| Fort Sanders Perinatal Center | Knoxville | — | $33.3M | 9 |
| ONE GENERATION AWAY | Franklin | R12 Civil Rights, Social Action & Advocacy | $32.5M | 1 |
| LANE COLLEGE | Jackson | B42Z Education | $32.4M | 2 |
| Nashville Symphony Association | Nashville | A690 Arts, Culture & Humanities | $32M | 12 |
| GIRLS PREPARATORY SCHOOL INC | Chattanooga | B200 Education | $31.7M | 6 |
| BRIARCREST CHRISTIAN SCHOOL SYSTEMS INC | Eads | B200 Education | $31.6M | 7 |
| Tennessee Aquarium | Chattanooga | D500 Animal-Related | $31.5M | 10 |
| JR HYDE III FAMILY FOUNDATION | Memphis | — | $31.5M | 3 |
| Credit Unions chartered in the State of Tennessee | Nashville | — | $31.4M | 12 |
| Le Bonheur Children's Hospital Foundation | Memphis | E112 Health Care | $31.4M | 12 |
| Porter-Leath | Memphis | — | $31.3M | 7 |
| SOUTHEAST LABORERS HEALTH FUND | Goodlettsville | — | $30.9M | 0 |
| LAUSANNE COLLEGIATE SCHOOL | Memphis | B21Z Education | $30.8M | 7 |
| PHILANTHROPIC IMPACT FOUNDATION | Franklin | — | $30.5M | 0 |
| Southern College of Optometry | Memphis | B500 Education | $30.5M | 7 |
| BAPTIST MEMORIAL COLLEGE OF HEALTH | Memphis | B500 Education | $30.2M | 16 |
| VULTEE CHARITABLE FOUNDATION | Nashville | — | $29.8M | 1 |
| THE MELKUS FAMILY FOUNDATION | Nashville | — | $29.8M | 1 |
| SECOND HARVEST FOOD BANK OF NORTHEAST TN | Kingsport | K310 Food, Agriculture & Nutrition | $29.3M | 1 |
| SOUTHEAST FINANCIAL CREDIT UNION | Franklin | — | $29.3M | 10 |
| METHODIST HEALTHCARE COMMUNITY CARE ASSOCIATES | Memphis | E22Z Health Care | $29.3M | 11 |
| BATTLE GROUND ACADEMY | Franklin | B25Z Education | $29.2M | 7 |
| CHATTANOOGA CHRISTIAN SCHOOL INC | Chattanooga | B200 Education | $29M | 6 |
| MEMPHIS CITY EMPLOYEES CREDIT UNION | Memphis | — | $29M | 8 |
| Bledsoe Telephone Cooperative Inc | Pikeville | — | $28.8M | 15 |
| ST MARY'S EPISCOPAL SCHOOL | Memphis | — | $28.6M | 7 |
| BILL AND CAROL LATIMER CHARITABLE | Union City | B82 Education | $28.5M | 0 |
| MIDDLE TENNESSEE STATE UNIVERSITY FOUNDATION | Murfreesboro | B110 Education | $28.4M | 0 |
| DOUGLAS CHEROKEE ECONOMIC | Morristown | B22Z Education | $28.3M | 2 |
| MUELLER INDUSTRIES INC BENEFITS TRUST | Collierville | — | $28.2M | 2 |
| THE DAY FOUNDATION | Memphis | — | $28M | 3 |
| MEMPHIS BUSINESS ACADEMY INC | Memphis | B29 Education | $27.9M | 8 |
| BRYAN COLLEGE | Dayton | B420 Education | $27.9M | 4 |
| LUKE INC | Collierville | L22 Housing & Shelter | $27.7M | 0 |
| DEKALB TELEPHONE COOPERATIVE INC | Alexandria | — | $27.7M | 19 |
| LeMoyne-Owen College | Memphis | B42Z Education | $27.5M | 9 |
| DOLLAR GENERAL LITERACY FOUNDATION | Goodlettsville | B92Z Education | $27.4M | 0 |
