| THE MULTIPLE MYELOMA RESEARCH | Norwalk | G50 Diseases, Disorders & Medical Disciplines | $29.1M | 15 |
| BOYS AND GIRLS VILLAGE INC | Milford | P730 Human Services | $29M | 8 |
| LOCAL 677 HEALTH SERVICE AND | Cheshire | — | $28.8M | 1 |
| CATHOLIC CHARITIES INC | Hartford | P40Z Human Services | $28.7M | 8 |
| UTC WELFARE BENEFITS PLAN | Farmington | Y43 Mutual & Membership Benefit | $28.5M | 1 |
| CANTERBURY SCHOOL INC | New Milford | B200 Education | $28.2M | 8 |
| MITCHELL COLLEGE | New London | B410 Education | $28.1M | 8 |
| BENHAVEN INC | North Haven | Z99Z Unknown | $27.6M | 7 |
| RECOVERY NETWORK OF PROGRAMS INC | Shelton | F20Z Mental Health & Crisis Intervention | $27.5M | 10 |
| NETWORK INC | Andover | S41I Community Improvement & Capacity Building | $26.8M | 8 |
| TEAM INC | Derby | P200 Human Services | $26.7M | 4 |
| SANDY HOOK PROMISE FOUNDATION | Newtown | I20 Crime & Legal-Related | $26.1M | 10 |
| NAFI CONNECTICUT INC | Hartford | — | $26.1M | 7 |
| THE CONNECTICUT COMMUNITY FOUNDATION | Waterbury | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $26.1M | 6 |
| THE HORACE BUSHNELL MEMORIAL HALL | Hartford | A610 Arts, Culture & Humanities | $26.1M | 10 |
| CONNECTICUT COALITION AGAINST DOMESTIC | Glastonbury | I71Z Crime & Legal-Related | $25.9M | 3 |
| Jerome Home | New Britain | — | $25.8M | 7 |
| St Vincent's Special Needs Center Inc | Trumbull | — | $25.8M | 14 |
| GENERATIONS FAMILY HEALTH CENTER | Willimantic | E30Z Health Care | $25.8M | 9 |
| CONNECTICUT JUNIOR REPUBLIC | Litchfield | — | $25.7M | 8 |
| CAPITAL WORKFORCE PARTNERS INC | Hartford | J200 Employment | $25.7M | 5 |
| CONNECTICUT BAPTIST HOMES INC | Meriden | P750 Human Services | $25.4M | 6 |
| THE ALTUM FUND | New Haven | — | $25.2M | 1 |
| Danbury Hospital & New Milford Hospital Foundation Inc | Danbury | E110 Health Care | $25.1M | 6 |
| The Board of Trustees of the Sheffield Scientific School | New Haven | B110 Education | $25M | 7 |
| WOODSTOCK ACADEMY | Woodstock | — | $24.9M | 6 |
| DUTCH POINT CREDIT UNION | Wethersfield | — | $24.9M | 9 |
| DELOITTE FOUNDATION | Stamford | T21 Philanthropy, Voluntarism & Grantmaking Foundations | $24.8M | 0 |
| THE FA BARTLETT TREE EXPERT COMPANY | Stamford | — | $24.7M | 0 |
| COMMUNITY SYSTEMS INC | Torrington | P82Z Human Services | $24.7M | 6 |
| ETHEL WALKER SCHOOL INC | Simsbury | B250 Education | $24.7M | 6 |
| Forman School Inc | Litchfield | — | $24.4M | 10 |
| CONNECTICUT HUMANITIES COUNCIL INC | Middletown | A700 Arts, Culture & Humanities | $24.4M | 1 |
| CONNECTICUT COMMUNITY CARE INC | Bristol | E920 Health Care | $24.3M | 6 |
| EAGLE HILL FOUNDATION INC | Greenwich | B280 Education | $24M | 10 |
| CHARTER OAK HEALTH CENTER INC | Hartford | E320 Health Care | $24M | 10 |
| THE ARC EASTERN CONNECTICUT INC | Norwich | — | $23.6M | 5 |
| SHATTERPROOF A NONPROFIT CORPORATION | Norwalk | F12 Mental Health & Crisis Intervention | $23.5M | 12 |
| HUMAN RESOURCES AGENCY OF NEW BRITAIN | New Britain | P200 Human Services | $23.4M | 3 |
| PEPPER WHISTON FOUNDATION | West Hartford | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $23.4M | 1 |
| IMRAN KHAN CANCER APPEAL INC | Farmington | — | $23.3M | 0 |
| CONNECTICUT PUBLIC BROADCASTING INC | Hartford | A300 Arts, Culture & Humanities | $22.9M | 12 |
| THE SOUTHEASTERN COUNCIL ON ALCOHOLISM | Norwich | F22Z Mental Health & Crisis Intervention | $22.6M | 7 |
| MOUNT SINAI REHABILITATION HOSPITAL | Hartford | E22 Health Care | $22.3M | 15 |
| THE CHESHIRE ACADEMY | Cheshire | — | $22.3M | 7 |
| BUILDON INC | Stamford | Q300 International, Foreign Affairs & National Security | $22.2M | 12 |
| MERIDEN-NEW BRITAIN-BERLIN YOUNG | Meriden | P27Z Human Services | $22.2M | 4 |
| MCCALL FOUNDATION INC | Torrington | — | $22.1M | 6 |
| INSTITUTE FOR NEURODEGENERATIVE | New Haven | H96 Medical Research | $22M | 12 |
| PRIMECARE INC | Waterbury | F33Z Mental Health & Crisis Intervention | $22M | 0 |
