| Scott & White Hospital-College Station | Dallas | E20 Health Care | $342.6M | 22 |
| Trinity University | San Antonio | B430 Education | $338.7M | 14 |
| Texas A&M Foundation | College Station | B43I Education | $338.7M | 10 |
| Texas Health Arlington Memorial Hospital | Arlington | E220 Health Care | $337.3M | 17 |
| UPLIFT EDUCATION | Dallas | B20Z Education | $332.6M | 13 |
| RALLY CREDIT UNION | Corpus Christi | — | $332.3M | 23 |
| ST LUKE'S COMMUNITY HEALTH SERVICES | Houston | E20 Health Care | $332M | 5 |
| BOY SCOUTS OF AMERICA | Irving | O410 Youth Development | $330.3M | 19 |
| SCHLUMBERGER GROUP HEALTH CARE TRUST | Houston | — | $325M | 1 |
| INTERNATIONAL LEADERSHIP OF TEXAS INC | Richardson | B99 Education | $322M | 8 |
| SOUTHERN HEALTHCARE ALLIANCE | Austin | F990 Mental Health & Crisis Intervention | $318.8M | 0 |
| Lake Pointe Operating Company LLC | Dallas | E22 Health Care | $313M | 14 |
| Permanent Endowment Fund of Moody Methodist Church | Galveston | X21 Religion-Related | $312.7M | 2 |
| Brazos Electric Power Cooperative Inc | Waco | — | $312.1M | 30 |
| Methodist Healthcare Ministries of South Texas Inc | San Antonio | E120 Health Care | $312.1M | 8 |
| Baylor Medical Center at Waxahachie | Dallas | E220 Health Care | $311M | 15 |
| BLUEBONNET ELECTRIC COOPERATIVE INC | Bastrop | — | $311M | 19 |
| MAGIC VALLEY ELECTRIC COOPERATIVE INC | Mercedes | — | $310.9M | 14 |
| Memorial Hermann Medical Group | Houston | E30 Health Care | $305.4M | 15 |
| Shannon Clinic | San Angelo | E30 Health Care | $299.9M | 25 |
| Texas Health Presbyterian Hospital Denton | Denton | E20 Health Care | $295.6M | 19 |
| O'DONNELL FOUNDATION | Dallas | — | $294.7M | 3 |
| RESPONSIVEED TEXAS | Lewisville | B20 Education | $291.3M | 5 |
| Mercy Ships International | Lindale | Q33 International, Foreign Affairs & National Security | $287.1M | 15 |
| YES Prep Public Schools Inc | Houston | B25P Education | $284.8M | 15 |
| University of the Incarnate Word | San Antonio | B430 Education | $282.3M | 17 |
| TARRANT SAFETY NET SUPPORT CORPORATION | Arlington | E19 Health Care | $278.1M | 3 |
| GRAYSON-COLLIN ELECTRIC COOPERATIVE INC | Van Alstyne | — | $261.6M | 20 |
| TCH Pediatric Associates Inc | Bellaire | E32 Health Care | $261.2M | 18 |
| COMMUNITIES FOUNDATION OF TEXAS INC | Dallas | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $249.3M | 20 |
| THE SAROFIM FOUNDATION | Houston | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $246.5M | 1 |
| EECU | Fort Worth | — | $246M | 28 |
| NORTH TEXAS FOOD BANK | Plano | — | $245.9M | 10 |
| GREATER HOUSTON COMMUNITY FOUNDATION | Houston | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $242.9M | 14 |
| SID PETERSON MEMORIAL HOSPITAL | Kerrville | E220 Health Care | $238.3M | 10 |
| Northeast Texas Electric Cooperative | Longview | — | $237.9M | 13 |
| CENTRAL TEXAS COMMUNITY HEALTH CENTERS | Austin | E193 Health Care | $234.9M | 17 |
| EL PASOANS FIGHTING HUNGER | El Paso | K31 Food, Agriculture & Nutrition | $233.3M | 1 |
| SAM HOUSTON ELECTRIC COOPERATIVE INC | Livingston | — | $232.2M | 26 |
| TML MULTISTATE INTERGOVERNMENTAL EMPLOYEE BENEFITS | Austin | Y44 Mutual & Membership Benefit | $229.1M | 5 |
| Florida Essential Healthcare Partnerships Foundation Inc | Austin | E12 Health Care | $227.4M | 0 |
| THE MUSEUM OF FINE ARTS HOUSTON | Houston | A510 Arts, Culture & Humanities | $226.8M | 11 |
| BOA RETIREE TRUST-BAC MEDICAL | Dallas | Y43 Mutual & Membership Benefit | $219.2M | 0 |
| SAN ANTONIO FOOD BANK | San Antonio | — | $217.3M | 6 |
| KOHLS DEPARTMNT STORES INC HEALTH & | Dallas | — | $217.2M | 1 |
| PRESERVATION FREEHOLD COMPANY | Lewisville | L20 Housing & Shelter | $215.3M | 6 |
| PURE HANDS INC | Plano | Q33 International, Foreign Affairs & National Security | $213.8M | 1 |
| EL PASO CHILDREN'S HOSPITAL CORPORATION | El Paso | E20 Health Care | $213M | 10 |
| HOUSTON LIVESTOCK SHOW AND RODEO INC | Houston | N520 Recreation & Sports | $212.8M | 10 |
| GOODWILL INDUSTRIES OF HOUSTON | Houston | J32Z Employment | $212.6M | 14 |
