| Carnegie Endowment for International Peace | Washington | Q402 International, Foreign Affairs & National Security | $86.8M | 16 |
| PARALYZED VETERANS OF AMERICA | Washington | H500 Medical Research | $86.4M | 22 |
| Aga Khan Foundation USA | Washington | Q300 International, Foreign Affairs & National Security | $85.8M | 7 |
| CARNEGIE INSTITUTION OF WASHINGTON | Washington | U310 Science & Technology | $85.6M | 14 |
| PaintCare Inc c/o American Coatings Association | Washington | C27 Environment | $84.7M | 10 |
| INTERNATIONAL ASSOCIATION OF FIRE FIGHTERS | Washington | — | $84.1M | 40 |
| GEORGETOWN DAY SCHOOL | Washington | B200 Education | $83.4M | 7 |
| INVESTMENT COMPANY INSTITUTE | Washington | — | $83.2M | 11 |
| EVIDENCE FOR IMPACT | Washington | — | $82.3M | 0 |
| DAVIS MEMORIAL GOODWILL INDUSTRIES | Washington | J32Z Employment | $81.3M | 8 |
| 50CAN INC | Washington | B012 Education | $80.6M | 9 |
| AMERICAN FEDERATION OF GOVERNMENT EMPLOYEES | Washington | J40 Employment | $80.2M | 22 |
| NATIONAL ASSOCIATION OF HOME BUILDERS OF THE UNITED STATES | Washington | — | $79.8M | 23 |
| SHARE OUR STRENGTH | Washington | Z99Z Unknown | $79.1M | 32 |
| AMERICA'S HEALTH INSURANCE PLANS INC | Washington | Y23 Mutual & Membership Benefit | $78.1M | 13 |
| BRICKLAYERS AND ALLIED CRAFTWORKERS INTERNATIONAL HEALTH FUND | Washington | — | $77.7M | 4 |
| MAG AMERICA INC | Washington | P20 Human Services | $77.4M | 3 |
| UNITED NATIONS FOUNDATION INC | Washington | Q114 International, Foreign Affairs & National Security | $77M | 8 |
| NATIONAL COUNCIL FOR BEHAVIORAL HEALTH | Washington | F030 Mental Health & Crisis Intervention | $76.7M | 12 |
| UNIDOSUS | Washington | Z99 Unknown | $75.9M | 11 |
| AMERICA VOTES | Washington | S20 Community Improvement & Capacity Building | $75.5M | 12 |
| RADIO FREE ASIA | Washington | A340 Arts, Culture & Humanities | $75.4M | 10 |
| NATIONAL RETAIL FEDERATION INC | Washington | — | $75.2M | 7 |
| LEAGUE OF CONSERVATION VOTERS EDUCATION FUND | Washington | C013 Environment | $75.2M | 6 |
| AB FOUNDATION | Washington | W05 Public & Societal Benefit | $74.6M | 4 |
| KIND INC | Washington | I80 Crime & Legal-Related | $74.4M | 10 |
| American Action Network Inc | Washington | P20 Human Services | $74.1M | 7 |
| HILLEL THE FOUNDATION FOR JEWISH CAMPUS LIFE | Washington | X30 Religion-Related | $73.2M | 10 |
| WHITE HOUSE HISTORICAL ASSOCIATION | Washington | A82Z Arts, Culture & Humanities | $72.9M | 13 |
| AMERICAN CENTER FOR INTERNATIONAL LABOR SOLIDARITY | Washington | — | $72.9M | 9 |
| NATIONAL ASSOCIATION OF BROADCASTERS | Washington | — | $71.5M | 12 |
| PENN TREATY PROTECTED CELL OF LTC RE | Washington | S40 Community Improvement & Capacity Building | $71.4M | 0 |
| CNFA | Washington | Q023 International, Foreign Affairs & National Security | $70.2M | 7 |
| ASSOCIATION OF AMERICAN RAILROADS | Washington | — | $70.2M | 11 |
| THE AMERICAN INSTITUTE OF ARCHITECTS | Washington | — | $70.1M | 10 |
| ATLANTIC COUNCIL OF THE UNITED STATES INC | Washington | Q055 International, Foreign Affairs & National Security | $69.9M | 14 |
| GLOBAL FISHING WATCH INC | Washington | C32 Environment | $69.5M | 9 |
| TELESCOPE FUND | Washington | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $69.4M | 0 |
| OPTICAL SOCIETY OF AMERICA INC | Washington | G030 Diseases, Disorders & Medical Disciplines | $68.2M | 8 |
| DC PREPARATORY ACADEMY | Washington | B29 Education | $68M | 13 |
| AMERICAN ENTERPRISE INSTITUTE FOR PUBLIC POLICY RESEARCH | Washington | V050 Social Science | $68M | 15 |
| NCTA - THE INTERNET & TELEVISION ASSOCIATION | Washington | — | $67.5M | 14 |
| LEAGUE OF CONSERVATION VOTERS INC | Washington | R01 Civil Rights, Social Action & Advocacy | $67.5M | 7 |
| AMERICAN CLEAN POWER ASSOCIATION | Washington | S41 Community Improvement & Capacity Building | $67.5M | 12 |
| DIGITAL PROMISE GLOBAL | Washington | Q20 International, Foreign Affairs & National Security | $66.8M | 14 |
| CHILDREN'S HOSPITAL FOUNDATION | Washington | E120 Health Care | $66.6M | 10 |
| RELIEF INTERNATIONAL INC | Washington | Q330 International, Foreign Affairs & National Security | $66.1M | 16 |
| AMERICAN POSTAL WORKERS UNION AFL-CIO | Washington | — | $65.7M | 89 |
| VOTER REGISTRATION PROJECT | Washington | — | $65.6M | 11 |
| THE MORRIS AND GWENDOLYN CAFRITZ FOUNDATION | Washington | T21 Philanthropy, Voluntarism & Grantmaking Foundations | $65.6M | 1 |
