| UAW RETIREES OF DAIMLER TRUCKS NORTH AMERICA WELFARE BENEFIT TRUST | Washington | Y43 Mutual & Membership Benefit | $65.6M | 7 |
| THE BUSINESS ROUNDTABLE INC | Washington | J99 Employment | $65.6M | 8 |
| COUNTERPART INTERNATIONAL INC | Washington | Q123 International, Foreign Affairs & National Security | $65.2M | 12 |
| ESCUELA AGRICOLA PANAMERICANA | Washington | Q310 International, Foreign Affairs & National Security | $65.2M | 8 |
| AMERICAN PSYCHIATRIC ASSOCIATION | Washington | S41 Community Improvement & Capacity Building | $64.6M | 24 |
| INTERNATIONAL ASSN OF BRIDGE STRUCTURAL ORNAMENTAL AND REINFORCING IRON WORKERS | Washington | — | $64.5M | 16 |
| Universities Space Research Association | Washington | U300 Science & Technology | $64.2M | 26 |
| Democracy Fund Inc | Washington | P20 Human Services | $64M | 4 |
| WASHINGTON DRAMA SOCIETY INC | Washington | A650 Arts, Culture & Humanities | $63.9M | 10 |
| SOCIETY FOR SCIENCE & THE PUBLIC | Washington | U030 Science & Technology | $63.6M | 15 |
| AMERICAN COLLEGE OF OBSTETRICIANS AND GYNECOLOGISTS | Washington | E03 Health Care | $63.5M | 49 |
| CATO INSTITUTE | Washington | W200 Public & Societal Benefit | $62.8M | 9 |
| SEIU HEALTH AND WELFARE FUND | Washington | — | $62M | 0 |
| NATIONAL ASSOCIATION OF MANUFACTURERS OF THE UNITED STATES OF AMERICA | Washington | — | $61.7M | 12 |
| JOINT BOARD OF TRUSTEES OF LOCAL 639 EMPLOYERS HEALTH TRUST FUND | Washington | — | $61.6M | 8 |
| MERIT AMERICA | Washington | P12 Human Services | $61.4M | 8 |
| SYRIAN AMERICAN MEDICAL SOCIETY FOUNDATION | Washington | E12 Health Care | $61.4M | 7 |
| AMERICAN FUEL AND PETROCHEMICAL MANUFACTURERS | Washington | — | $60.8M | 10 |
| BREAKTHROUGH PRIZE FOUNDATION | Washington | B82 Education | $60.8M | 3 |
| AVINA AMERICAS INC | Washington | Q30 International, Foreign Affairs & National Security | $60.7M | 5 |
| CENTER FOR STRATEGIC AND INTERNATIONAL STUDIES | Washington | Q50Z International, Foreign Affairs & National Security | $59.8M | 16 |
| MSI US | Washington | T11 Philanthropy, Voluntarism & Grantmaking Foundations | $59.8M | 6 |
| NORTH FUND | Washington | W20 Public & Societal Benefit | $59.7M | 1 |
| MORTGAGE BANKERS ASSOCIATION | Washington | Z99 Unknown | $59.7M | 18 |
| NUCLEAR ENERGY INSTITUTE INC | Washington | — | $59.4M | 13 |
| AMERICAN ISRAEL EDUCATION FOUNDATION INC | Washington | Q200 International, Foreign Affairs & National Security | $59.2M | 3 |
| CATHOLIC CHARITIES OF THE ARCHDIOCESE OF WASHINGTON | Washington | P20Z Human Services | $59.1M | 11 |
| CERTIFIED FINANCIAL PLANNER BOARD OF STANDARDS INC | Washington | S41 Community Improvement & Capacity Building | $58.7M | 13 |
| THE AMERICAN INDEPENDENT FOUNDATION | Washington | Z99 Unknown | $58.6M | 8 |
| THE VOTER PARTICIPATION CENTER | Washington | R40 Civil Rights, Social Action & Advocacy | $58M | 7 |
| GUARANTY ASSOCIATION BENEFITS COMPANY | Washington | S41 Community Improvement & Capacity Building | $57.7M | 3 |
| GREEN BUSINESS CERTIFICATION INC | Washington | S40 Community Improvement & Capacity Building | $57.6M | 6 |
| COMMUNITY OF HOPE | Washington | S200 Community Improvement & Capacity Building | $57.6M | 12 |
| AMERICAN GEOPHYSICAL UNION | Washington | U300 Science & Technology | $56.2M | 22 |
| MUNICIPAL SECURITIES RULEMAKING BOARD | Washington | — | $56.1M | 44 |
| NEW ISRAEL FUND | Washington | Q300 International, Foreign Affairs & National Security | $55.8M | 10 |
| ELECTRONIC PAYMENTS COALITION INC | Washington | Y03 Mutual & Membership Benefit | $55.6M | 2 |
| METROPOLITAN WASHINGTON COUNCIL OF GOVERNMENTS | Washington | W200 Public & Societal Benefit | $55.2M | 16 |
| INTERNATIONAL UNION OF BRICKLAYERS & ALLIED CRAFTWORKERS | Washington | — | $54.9M | 10 |
| DUTY AND HONOR | Washington | — | $54.4M | 0 |
| PHARMACEUTICAL CARE MANAGEMENT ASSOCIATION | Washington | — | $54.2M | 15 |
| AMERICAN COUNCIL OF LIFE INSURERS | Washington | — | $53.4M | 14 |
| WASHINGTON DC CONVENTION & TOURISM CORPORATION | Washington | S41 Community Improvement & Capacity Building | $53.2M | 7 |
| TRUTH INITIATIVE FOUNDATION | Washington | F210 Mental Health & Crisis Intervention | $53M | 10 |
| INNOVATIONS FOR POVERTY ACTION A NONPROFIT CORPORATION | Washington | V30 Social Science | $52.8M | 8 |
| Center on Budget and Policy Priorities | Washington | W050 Public & Societal Benefit | $52.5M | 10 |
| AMERICAN FARMLAND TRUST | Washington | K250 Food, Agriculture & Nutrition | $52.1M | 10 |
| CENTER FOR INTERNATIONAL PRIVATE ENTERPRISE INC | Washington | Q320 International, Foreign Affairs & National Security | $52M | 12 |
| UNITED MINE WORKERS OF AMERICA COMBINED BENEFIT FUND | Washington | — | $52M | 7 |
| NATIONAL MARINE MANUFACTURERS ASSOCIATION | Washington | — | $51.7M | 21 |
