| ST JOHN FISHER UNIVERSITY | Rochester | B430 Education | $160.2M | 18 |
| MOLLOY UNIVERSITY | Rockville Centre | B420 Education | $159.2M | 13 |
| UNITED ISRAEL APPEAL INC | New York | Q710 International, Foreign Affairs & National Security | $157.6M | 2 |
| WASHINGTON-SARATOGA-WARREN-HAMILTON | Hudson Falls | Y44 Mutual & Membership Benefit | $157.2M | 0 |
| NATIONAL AUDUBON SOCIETY INC | New York | C300 Environment | $156.1M | 13 |
| NORTHWELL HEALTH FOUNDATION | Westbury | E122 Health Care | $155.8M | 20 |
| ICIRCLE SERVICES OF THE FINGER | Webster | P82 Human Services | $153.8M | 11 |
| AMERICU CREDIT UNION | Rome | — | $153.3M | 10 |
| The Royal National Theatre | New York | A65 Arts, Culture & Humanities | $152.4M | 8 |
| AMERICAN ARBITRATION ASSOCIATION INC | New York | I510 Crime & Legal-Related | $151.3M | 14 |
| Westhab Inc | Yonkers | L410 Housing & Shelter | $151.3M | 17 |
| RAYIM OF HUDSON VALLEY INC | Monroe | P82 Human Services | $151M | 7 |
| ST JOSEPH'S MEDICAL PC | Syracuse | E300 Health Care | $151M | 15 |
| DORIS DUKE CHARITABLE FOUNDATION INC | New York | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $150.5M | 5 |
| DELIVERING GOOD INC | New York | P190 Human Services | $149.9M | 6 |
| Mount St Mary's Hospital | Buffalo | E220 Health Care | $149.6M | 21 |
| NATIONAL MULTIPLE SCLEROSIS SOCIETY | New York | G500 Diseases, Disorders & Medical Disciplines | $149.5M | 13 |
| ACTION AGAINST HUNGER - USA | New York | Q300 International, Foreign Affairs & National Security | $149.2M | 11 |
| The Winifred Masterson Burke Rehabilitation Hospital | White Plains | E20 Health Care | $149.2M | 11 |
| KIPP NYC PUBLIC CHARTER SCHOOLS | New York | B29 Education | $149M | 15 |
| HOBART AND WILLIAM SMITH COLLEGES | Geneva | B420 Education | $148.6M | 13 |
| HERITAGE CHRISTIAN SERVICES INC | Rochester | P820 Human Services | $147.9M | 10 |
| ASCEND CHARTER SCHOOLS | Brooklyn | B29 Education | $147.5M | 5 |
| DEVELOPMENTAL DISABILITIES INSTITUTE INC | Smithtown | P820 Human Services | $147.3M | 9 |
| THE COOPER UNION FOR THE | New York | B420 Education | $147.3M | 14 |
| NEW YORK STATE HEALTH FDN | New York | — | $145.1M | 1 |
| ADIRONDACK MEDICAL CENTER | Saranac Lake | E220 Health Care | $145M | 14 |
| FRIENDS OF UNITED HATZALAH INC | New York | A70 Arts, Culture & Humanities | $144.9M | 7 |
| WELFARE FUND OF THE IUOE LOCAL UNIONS | Long Island City | — | $144.9M | 6 |
| CAYUGA HOME FOR CHILDREN | Auburn | P730 Human Services | $144.8M | 23 |
| SUCCESS ACADEMY CHARTER SCHOOLS INC | New York | B990 Education | $143.9M | 12 |
| THE CHILDREN'S VILLAGE INC | Dobbs Ferry | P730 Human Services | $143.4M | 7 |
| UNITED MEDICAL ASSOCIATES PC | Vestal | E32Z Health Care | $143.3M | 23 |
| DRUCKENMILLER FOUNDATION | New York | — | $143.2M | 0 |
| CLIFTON SPRINGS SANITARIUM CO | Clifton Springs | E22 Health Care | $143.1M | 16 |
| CLAXTON-HEPBURN MEDICAL CENTER | Ogdensburg | E220 Health Care | $143.1M | 15 |
| Hadassah The Women's Zionist Org of America Inc | New York | S810 Community Improvement & Capacity Building | $142.9M | 9 |
| COMMUNITY HEALTH AIDE SERVICES INC | Montebello | E92Z Health Care | $142.9M | 10 |
| Global Action to End Smoking Inc | New York | S05 Community Improvement & Capacity Building | $142.8M | 10 |
| AMERICAN CIVIL LIBERTIES UNION INC | New York | — | $142.7M | 15 |
| HILLSIDE CHILDREN'S CENTER | Rochester | P300 Human Services | $142.6M | 7 |
| AMERICAN FRIENDS OF MAGEN DAVID ADOM | New York | Q113 International, Foreign Affairs & National Security | $142.3M | 11 |
| GRAMEEN AMERICA INC | Jackson Heights | S31 Community Improvement & Capacity Building | $141.8M | 11 |
| THE CENTER FOR DISCOVERY INC | Harris | G200 Diseases, Disorders & Medical Disciplines | $141.7M | 9 |
| USTA NATIONAL TENNIS CENTER INCORPORATED | Purchase | N660 Recreation & Sports | $140.8M | 7 |
| NEIGHBORHOOD ASSOCIATION FOR | Bronx | — | $140.5M | 13 |
| GIVEDIRECTLY INC | New York | P60 Human Services | $140.3M | 11 |
| LOCAL 804 WELFARE TRUST FUND | Port Washington | — | $138.8M | 11 |
| ST BONAVENTURE UNIVERSITY | St Bonaventure | — | $138.7M | 15 |
| TNTP INC | New York | B90 Education | $138.6M | 14 |
