| SURDNA FOUNDATION INC | New York | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $193.4M | 7 |
| NATURAL RESOURCES DEFENSE COUNCIL INC | New York | C300 Environment | $193.1M | 16 |
| HealthAlliance Hospital | Kingston | E20 Health Care | $189.4M | 18 |
| INSTITUTE FOR COMMUNITY LIVING INC | New York | F33 Mental Health & Crisis Intervention | $188.6M | 8 |
| SESAME WORKSHOP | New York | A320 Arts, Culture & Humanities | $186.9M | 13 |
| ST FRANCIS COLLEGE | Brooklyn | B420 Education | $186.9M | 11 |
| MOTHER CABRINI HEALTH FOUNDATION INC | New York | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $185.2M | 7 |
| AMERICAN CIVIL LIBERTIES UNION FOUNDATION | New York | R600 Civil Rights, Social Action & Advocacy | $185.1M | 15 |
| HAMASPIK OF KINGS COUNTY INC | Brooklyn | P70I Human Services | $184.9M | 6 |
| OSWEGO HOSPITAL | Oswego | E220 Health Care | $184.8M | 10 |
| DOMINION ENERGY INC COLLECTIVELY | New York | — | $184.6M | 1 |
| NEW WATER STREET CORP | New York | — | $184.4M | 9 |
| Iona University | New Rochelle | B43 Education | $183.3M | 13 |
| CORNING HOSPITAL | Corning | E220 Health Care | $183.1M | 15 |
| AUBURN COMMUNITY HOSPITAL | Auburn | E220 Health Care | $183.1M | 9 |
| BOWERY RESIDENTS COMMITTEE INC | New York | P200 Human Services | $182.4M | 8 |
| MAJOR LEAGUE BASEBALL PLAYERS | New York | — | $182.3M | 9 |
| JUILLIARD SCHOOL | New York | B430 Education | $181.2M | 20 |
| ST PETER'S HEALTH PARTNERS | Albany | E21 Health Care | $181.1M | 16 |
| ROBIN HOOD FOUNDATION | New York | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $180.5M | 13 |
| UNITED MEMORIAL MEDICAL CENTER | Batavia | E22Z Health Care | $180.1M | 14 |
| NEW YORK STATE UNITED TEACHERS | Latham | — | $176.8M | 105 |
| 1199SEIU NBF FOR HOME CARE EMPLOYEES | New York | Y43 Mutual & Membership Benefit | $176.4M | 4 |
| NYC SCHOOL BUS UMBRELLA SERVICES INC | Brooklyn | W40 Public & Societal Benefit | $174.4M | 7 |
| HUMAN CARE SERVICES FOR FAMILIES & CHILDREN | Brooklyn | E920 Health Care | $173.7M | 10 |
| THE CHILDREN'S AID SOCIETY | New York | P300 Human Services | $173.2M | 10 |
| THE SIMONS FOUNDATION INC | New York | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $171.4M | 11 |
| UPMC CHAUTAUQUA AT WCA | Jamestown | E220 Health Care | $171.3M | 12 |
| FAMILY RESIDENCES AND ESSENTIAL | Old Bethpage | F330 Mental Health & Crisis Intervention | $169.2M | 14 |
| Montefiore New Rochelle Hospital | New Rochelle | E22 Health Care | $167.9M | 10 |
| NEO Philanthropy Inc | New York | W99Z Public & Societal Benefit | $167.6M | 12 |
| AMERICAN FRIENDS OF HEBREW | New York | Q113 International, Foreign Affairs & National Security | $167.5M | 11 |
| NYSARC INC Nassau County Chapter | Brookville | P820 Human Services | $167.3M | 11 |
| THE INSTITUTE FOR FAMILY HEALTH | New York | E320 Health Care | $167.1M | 17 |
| Le Moyne College | Syracuse | B420 Education | $166.5M | 10 |
| CONCEPTS OF INDEPENDENCE INC | New York | — | $165.8M | 7 |
| FOOD BANK FOR NEW YORK CITY | New York | S50 Community Improvement & Capacity Building | $164.3M | 7 |
| ST MARY'S HEALTHCARE | Amsterdam | E220 Health Care | $164.2M | 13 |
| THE QUEENS BOROUGH PUBLIC LIBRARY | Jamaica | B700 Education | $163.6M | 12 |
| THE FEINSTEIN INSTITUTES FOR | Westbury | H900 Medical Research | $163.3M | 10 |
| CSEA EMPLOYEE BENEFIT FUND | Latham | — | $163.3M | 3 |
| BROOKLYN PUBLIC LIBRARY | Brooklyn | B71Z Education | $163.2M | 9 |
| WOMEN IN NEED INC | New York | P83Z Human Services | $163.1M | 12 |
| WSNCHS NORTH INC | Bethpage | E220 Health Care | $162.5M | 10 |
| UNCOMMON NEW YORK CITY CHARTER SCHOOLS | New York | B29 Education | $162.2M | 15 |
| NIAGARA UNIVERSITY | Niagara University | B430 Education | $161.7M | 8 |
| THE ROXANNE AND HENRY BRANDT FOUNDATION | New York | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $160.6M | 0 |
| THE AMERICAN SOCIETY OF MECHANICAL | New York | U030 Science & Technology | $160.5M | 10 |
| CHRISTO AND JEANNE-CLAUDE FOUNDATION | New York | A51 Arts, Culture & Humanities | $160.4M | 2 |
| THE CIVIL SERVICE EMPLOYEES ASSOCIATION | Albany | — | $160.3M | 72 |
