| HORIZON EDUCATION CENTERS | North Olmsted | — | $24M | 1 |
| TRINITY HOSPITAL TWIN CITY | Dennison | — | $23.9M | 12 |
| WELLINGTON SCHOOL | Columbus | — | $23.8M | 7 |
| THE DYNAMIC CATHOLIC INSTITUTE | Cincinnati | X22 Religion-Related | $23.8M | 8 |
| LEGAL AID OF SOUTHEAST AND CENTRAL OHIO | Columbus | I800 Crime & Legal-Related | $23.7M | 1 |
| SETON EDUCATION PARTNERS | Cincinnati | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $23.7M | 5 |
| CINCINNATI MUSEUM ASSOCIATION | Cincinnati | A510 Arts, Culture & Humanities | $23.7M | 7 |
| SECOND HARVEST FOOD BANK OF THE MAHONING VALLEY | Youngstown | K31Z Food, Agriculture & Nutrition | $23.6M | 1 |
| DAYTON CHILDREN'S HOSPITAL FOUNDATION | Dayton | E110 Health Care | $23.6M | 4 |
| CHMC COMMUNITY HEALTH SERVICES NETWORK | Cincinnati | E32 Health Care | $23.5M | 9 |
| CLEVELAND MUSEUM OF NATURAL HISTORY | Cleveland | A560 Arts, Culture & Humanities | $23.5M | 10 |
| CENTRAL OHIO HEALTH CARE CONSORTIUM | Granville | — | $23.5M | 0 |
| CHILDREN'S HOME CARE OF DAYTON | Dayton | E92Z Health Care | $23.4M | 10 |
| GREATER COLUMBUS CONVENTION AND VISITORS BUREAU INC | Columbus | — | $23.3M | 7 |
| REUBEN MCMILLAN FREE LIBRARY ASSOCIATION | Youngstown | B700 Education | $23.2M | 5 |
| LUTHER HOME OF MERCY | Williston | P730 Human Services | $23.2M | 3 |
| IUPAT WELFARE FUND OF WESTERN PENNSYLVANIA | Canfield | Y43 Mutual & Membership Benefit | $23.1M | 0 |
| PROMEDICA NORTHWEST OHIO CARDIOLOGY CONSULTANTS | Toledo | E30 Health Care | $23.1M | 12 |
| CHILD FOCUS | Cincinnati | P300 Human Services | $23.1M | 6 |
| COMMQUEST SERVICES INC | Canton | P462 Human Services | $23.1M | 8 |
| Ohio North East Health Systems Inc | Youngstown | E21 Health Care | $23M | 19 |
| CHILDREN'S HUNGER ALLIANCE | Columbus | K40Z Food, Agriculture & Nutrition | $22.9M | 5 |
| STARK COUNTY DISTRICT LIBRARY | Canton | B71I Education | $22.8M | 2 |
| ROBERT ADELE SCHIFF FAMILY FDN INC | Cincinnati | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $22.8M | 0 |
| PLUMBERS & STEAMFITTERS #189 WELFARE FUND | Columbus | — | $22.7M | 3 |
| CINCINNATI USA REGIONAL CHAMBER | Cincinnati | — | $22.7M | 9 |
| OHIO STATE PLUMBERS & PIPEFITTERS HEALTH AND WELFARE FUND | Marietta | — | $22.7M | 0 |
| PIPE FITTERS LOCAL 120 INSURANCE FUND | Cleveland | — | $22.6M | 3 |
| VICTORIA THEATRE ASSOCIATION | Dayton | A610 Arts, Culture & Humanities | $22.6M | 8 |
| MAKE-A-WISH FOUNDATION OF OH KY & IN | Columbus | E86Z Health Care | $22.6M | 8 |
| Euclid Avenue Development Corporation | Cleveland | B19 Education | $22.6M | 3 |
| MOUNT CARMEL COLLEGE OF NURSING | Columbus | — | $22.5M | 10 |
| METHODIST RETIREMENT CENTER OF CENTRAL OHIO | Columbus | P190 Human Services | $22.5M | 5 |
| THIRD STREET COMMUNITY CLINIC | Mansfield | — | $22.4M | 12 |
| BUCKEYE COMMUNITY SERVICES INC | Jackson | P82Z Human Services | $22.4M | 1 |
| SHAREFAX CREDIT UNION INC | Cincinnati | — | $22.2M | 4 |
| St Joseph Infant and Maternity Home | Cincinnati | P730 Human Services | $22.2M | 5 |
| Heart of Ohio Family Health Centers | Columbus | E320 Health Care | $22.1M | 10 |
| APPLEWOOD CENTERS INC | Shaker Heights | Z99 Unknown | $22.1M | 8 |
| ENA INC | Cincinnati | P320 Human Services | $22M | 0 |
| CROSSROADS HEALTH | Mentor | F30Z Mental Health & Crisis Intervention | $22M | 8 |
| THE FOUNDATION OF THE CATHOLIC DIOCESE OF COLUMBUS | Columbus | X112 Religion-Related | $21.9M | 5 |
| The Huntington Foundation | Columbus | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $21.9M | 0 |
| CHARLES K KING NO 3 TRUST CO KEYBANK NATIONAL ASSOCIATION | Brooklyn | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $21.9M | 7 |
| FRIENDSHIP VILLAGE OF COLUMBUS OHIO INC | Columbus | P750 Human Services | $21.8M | 6 |
| VALLEY HOSPICE INC | Rayland | — | $21.8M | 1 |
| FRONTIER COMMUNITY SERVICES | Chillicothe | P820 Human Services | $21.5M | 3 |
| FARMER FAMILY FOUNDATION | Cincinnati | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $21.4M | 0 |
| IDEA GREATER CINCINNATI INC | Cincinnati | B20 Education | $21.4M | 0 |
| CENTRAL CLINIC - GROUP | Cincinnati | — | $21.4M | 8 |
